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Plant Systematics and Evolution

, Volume 304, Issue 5, pp 679–697 | Cite as

A multivariate morphometric delimitation of species boundaries in the South American genus Nicoraepoa (Poaceae: Pooideae: Poeae)

  • Victor L. Finot
  • Robert J. Soreng
  • Liliana M. Giussani
  • Romina G. Muñoz
Original Article

Abstract

A multivariate morphometric study of the genus Nicoraepoa was carried out to examine patterns of morphological variation among the species and to identify additional characters to allow a clearer delimitation among species. Nicoraepoa is a taxonomically complex genus comprising seven species endemic to South America. Although most of the species are well delimited, two of them, N. andina and N. chonotica, are morphologically similar and very difficult to separate. The morphological variation among species, with overlapping morphological characters, pseudovivipary and possible hybridization make species boundaries unclear. In the present paper, sixty-seven characters were scored for a total of 216 specimens and analyzed using ordination, cluster and discriminant analyses. Based on multivariate results, we recognized species groups and morphological diagnostic characters that allow clearer species delimitation. As a result, N. chonotica has been transferred to N. andina as a subspecies that inhabit southern Patagonia. Other taxa could be recognized and delimited by diagnostic characters: N. erinacea, N. pugionifolia, N. stepparia, N. subenervis subsp. spegazziniana and N. subenervis subsp. subenervis. Moreover, we discuss the possibility that a new species from Bolivia, based on only one specimen, closely related to N. subenervis should be ascribed in Nicoraepoa.

Keywords

Argentina Bolivia Chile Multivariate analysis Southern Patagonia Species delimitation 

Notes

Acknowledgments

Authors sincerely thank the Directors and Curators of the herbaria BAA, CONC, LPB, SI, SGO and US for loan specimens for this study and sending digital images of the types. Universidad de Concepción, Smithsonian Institution, and Instituto de Botánica Darwinion are thanked for logistical support; Paul Peterson for RJS field work in Argentina in 2003 and 2006. We also thank Paola Martínez and Nicolás Villalobos for technical support. Thanks are due to two anonymous reviewers for comments and suggestions to improve the manuscript.

Supplementary material

606_2018_1499_MOESM1_ESM.pdf (188 kb)
Supplementary material 1 (PDF 188 kb)
606_2018_1499_MOESM2_ESM.pdf (63 kb)
Supplementary material 2 (PDF 63 kb)
606_2018_1499_MOESM3_ESM.pdf (96 kb)
Supplementary material 3 (PDF 95 kb)

References

  1. Cabi E, Soreng RJ, Gillespie LJ, Amiri N (2016) Poa densa (Poaceae), an overlooked Turkish steppe grass, and the evolution of bulbs in Poa. Willdenowia 46:201–211.  https://doi.org/10.3372/wi.46.46201 CrossRefGoogle Scholar
  2. Dayrat B (2005) Towards integrative taxonomy. Biol J Linn Soc 85:407–415.  https://doi.org/10.1111/j.1095-8312.2005.00503.x CrossRefGoogle Scholar
  3. Di Rienzo JA, Casanoves F, Balzarini MG, Gonzalez L, Tablada M, Robledo CW (2016) InfoStat versión 2016. Grupo InfoStat, FCA, Universidad Nacional de Córdoba, Córdoba. Available at: http://www.infostat.com.ar/
  4. Ellis RP (1976) A procedure for standardizing comparative leaf anatomy in the Poaceae, I. The leaf blade as viewed in transverse section. Bothalia 12:65–109CrossRefGoogle Scholar
  5. Elmqvist T, Cox PA (1996) The evolution of vivipary in flowering plants. Oikos 77:3–9CrossRefGoogle Scholar
  6. Everitt BS, Landau S, Leese M, Stahl D (2011) Cluster analysis. 5th edn. Wiley, ePDF by Thomson Digital, NoidaGoogle Scholar
  7. Finot VL, Barrera JA, Marticorena C, Rojas G (2011) Systematic diversity of the family Poaceae (Gramineae) in Chile. In: Grillo O, Venora G (eds) The dynamical processes of biodiversity-case studies of evolution and spatial distribution. In-Tech Open Access, Rijeka, pp 71–108.  https://doi.org/10.5772/23346 Google Scholar
  8. Gillespie LJ, Soreng RJ (2005) A phylogenetic analysis of the Bluegrass genus Poa based on cpDNA restriction site data. Syst Bot 30:84–105.  https://doi.org/10.1600/0363644053661940 CrossRefGoogle Scholar
  9. Gillespie LJ, Soreng RJ, Bull RD, Jacobs SWL, Refulio-Rodríguez NF (2008) Phylogenetic relationships in subtribe Poinae (Poaceae, Poeae) based on nuclear ITS and plastid trnT-trnL-trnF sequences. Botany 86:938–967.  https://doi.org/10.1139/B08-076 CrossRefGoogle Scholar
  10. Gillespie LJ, Soreng RJ, Jacobs WL (2009) Phylogenetic relationships of Australian Poa (Poaceae: Poinae), including molecular evidence for two new genera, Saxipoa and Sylvipoa. Austral Syst Bot 22:413–436CrossRefGoogle Scholar
  11. Gillespie LJ, Soreng RJ, Paradis M, Bull RD (2010) Phylogeny and reticulation in subtribe Poinae and related subtribes (Poaceae) based on nrITS, ETS, and trnTLF data. In: Seberg O, Petersen G, Barfod AS, Davis JI (eds) Diversity, phylogeny, evolution Monocot. Aarhus University Press, Aarhus, pp 589–618Google Scholar
  12. Giussani LM, Soreng RJ, Anton AM (2011) Novedades nomenclaturales en Poaceae Argentinas. Darwiniana 49:90–93Google Scholar
  13. Giussani LM, Gillespie LJ, Scataglini MA, Negritto MA, Anton AM, Soreng RJ (2016) Breeding system diversification and evolution in American Poa supersect. Homalopoa (Poaceae: Poeae: Poinae). Ann Bot (Oxford) 118:281–303.  https://doi.org/10.1093/aob/mcw108 CrossRefGoogle Scholar
  14. Gower JC (1966) Some distance properties of latent root and vector methods used in multivariate analysis. Biometrika 53:325–338CrossRefGoogle Scholar
  15. Gower JC (1971) A general coefficient of similarity and some of its properties. Biometrics 27:857–872CrossRefGoogle Scholar
  16. Gower JC, Legendre P (1986) Metric and Euclidean properties of dissimilarity coefficients. J Classific 5:5–48CrossRefGoogle Scholar
  17. Mahibbur RM, Govindarajulu Z (1997) A modification of the test of Shapiro and Wilks for normality. J Appl Statist 24:219–235CrossRefGoogle Scholar
  18. Marhold K (2011) Multivariate morphometrics and its application to monography at specific and infraspecific levels. In: Stuessy TF, Lack HW (eds) Monographic plant systematics: fundamental assessment of plant biodiversity. Gantner Verlag, Ruggel, pp 75–101Google Scholar
  19. Metcalfe CR (1960) Anatomy of the monocotyledons. I. Gramineae. Oxford University Press, LondonGoogle Scholar
  20. Moore DM, Dogett MD (1976) Pseudo-vivipary in Fuegian and Falkland Island grasses. Brit Antarct Surv Bull 43:100–110Google Scholar
  21. Nicora EG (1977) Gramíneas argentinas nuevas. Hickenia 1:99–107Google Scholar
  22. Nicora EG (1978) Gramineae. In: Correa MN (ed) Flora Patagónica. Colección Científica del Instituto Nacional de Tecnología Agropecuaria 8(3), Buenos Aires, pp 1–563Google Scholar
  23. Pierce S, Stirling CM, Baxter R (2003) Pseudoviviparous reproduction of Poa alpina var. vivipara L. (Poaceae) during long-term exposure to elevated atmospheric CO2. Ann Bot (Oxford) 91:613–622.  https://doi.org/10.1093/aob/mcg067 CrossRefGoogle Scholar
  24. Sassone AB, Giussani LM, Guaglianone ER (2013) Multivariate studies of Ipheion (Amaryllidaceae, Allioideae) and related genera. Pl Syst Evol 8:1561–1575.  https://doi.org/10.1007/s00606-013-0819-5 CrossRefGoogle Scholar
  25. Soreng RJ, Gillespie LJ (2007) Nicoraepoa (Poaceae, Poeae), a new South American genus based on Poa subg. Andinae, and emendation of Poa sect. Parodiochloa of the Sub-Antarctic islands. Ann Missouri Bot Gard 94:821–849.  https://doi.org/10.3417/0026-6493 CrossRefGoogle Scholar
  26. Soreng RJ, Giussani LM (2012) Nicoraepoa Soreng & Gillespie. In: Zuloaga FO, Rúgolo ZE, Anton AM (eds) Flora Argentina (II). Gráficamente Ediciones, Córdoba, pp 270–277Google Scholar
  27. Soreng RJ, Peterson PM (2008) New records of Poa (Poaceae) and Poa pfisteri: a new species endemic to Chile. J Bot Res Inst Texas 2:847–859Google Scholar
  28. Soreng RJ, Gillespie LJ, Koba H, Boudko E, Bull RD (2015a) Molecular and morphological evidence for a new genus Dupontiopsis (Poaceae tribe Poeae subtribe Poinae s.l.), endemic to alpine Japan, and implications for the reticulate origin of Dupontia and Arctophila within Poinae s.l. J Syst Evol 53:138–162.  https://doi.org/10.1111/jse.12146 CrossRefGoogle Scholar
  29. Soreng RJ, Peterson PM, Romaschenko K, Davidse G, Zuloaga FO, Judziewicz EJ, Filgueiras TS, Davis JL, Morrone O (2015b) A worldwide phylogenetic classification of the Poaceae (Gramineae). J Syst Evol 53:117–137.  https://doi.org/10.1111/jse.1215 CrossRefGoogle Scholar
  30. Thiers B (continuously updated) Index Herbariorum: a global directory of public herbaria and associated staff. New York Botanical Garden’s Virtual Herbarium. New York Botanical Garden, Bronx. Available at: http://sweetgum.nybg.org/science/ih/. Accessed 9 Sep 2016
  31. Zuloaga FO, Morrone O, Belgrano M (2008) Catálogo de las plantas vasculares del Cono Sur. Monographs in Systematic Botany 107. Missouri Botanical Garden Press, St Louis. Available at: http://www.darwin.edu.ar/Proyectos/FloraArgentina/Familias.asp. Accessed 28 Nov 2016

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  • Victor L. Finot
    • 1
  • Robert J. Soreng
    • 2
  • Liliana M. Giussani
    • 3
  • Romina G. Muñoz
    • 1
  1. 1.Departamento de Producción Animal, Facultad de AgronomíaUniversidad de ConcepciónChillánChile
  2. 2.Department of Botany, United States National Herbarium, National Museum of Natural HistorySmithsonian InstitutionWashingtonUSA
  3. 3.Instituto de Botánica DarwinionBuenos AiresArgentina

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