Molecular imprinting resonance light scattering nanoprobes based on pH-responsive metal-organic framework for determination of hepatitis A virus


Molecularly imprinted polymer (HM@MIP) nanoprobes were designed form the pH-responsive polymer (dimethylaminoethyl methacrylate (DMA)) and MIL-101. This probe was applied to the selective determination of hepatitis A virus (HAV) through Resonance light scattering (RLS) technique. DMA adjusts pH of the system to facilitate the capture and release of virus by HM@MIPs as anticipated. And it results in the enhancement or weaken of RLS intensity. According to RLS intensity at 470 nm, a linear concentration of 0.02–2.0 nmol·L−1 and a limit of detection of 0.1 pmol·L−1 were obtained within 20 min. The excellent recoveries ranges from 88% to 107%, and it indicates the prominent ability of the HM@MIPs to determination HAV in human serum and their potential ability to determination virus in real applications.

Principle of preparation of the HM@MIPs and detection of virus.

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This work was supported by the National Natural Science Foundation of China (No. 21775132), the National Natural Science Foundation of Hunan province (No.2018JJ2388). Hunan 2011 Collaborative Innovation Center of Chemical Engineering & Technology with Environmental Benignity and Effective Resource Utilization, the project of innovation team of the ministry of education (IRT_17R90).

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Correspondence to Changqun Cai.

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Luo, L., Zhang, F., Chen, C. et al. Molecular imprinting resonance light scattering nanoprobes based on pH-responsive metal-organic framework for determination of hepatitis A virus. Microchim Acta 187, 140 (2020).

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  • MIL-101
  • Resonance light scattering nanoprobe
  • pH- responsive polymer
  • Virus molecular imprinting