Abstract
Carbon quantum dots co-doped with iron and nitrogen (Fe@NCDs) were synthesized by using Passiflora edulis Sims (P. edulis) as a precursor. The Fe@NCDs exhibit outstanding peroxidase-mimetic activity owing to successful doping with iron resulting in a behavior similar to that of iron porphyrins. In the presence of H2O2, the Fe@NCDs catalyze the oxidation of the peroxidase substrate 3,3′,5,5′-tetramethylbenzidine (TMB) with a color change from colorless to blue. The blue oxidation product has a characteristic absorption peaking at 652 nm. A colorimetric assay was worked out for uric acid (UA) that measures the hydrogen peroxide produced during oxidation of UA by uricase. Response is linear in the 2–150 μM UA concentration range, and the limit of detection is 0.64 μM. The method was applied to the determination of UA in (spiked) urine, and recoveries ranged from 92.0 to 103.4%.
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This work was supported by the National Natural Science Foundation of China (No. 61664002) and Guangxi major science and technology projects (AA18242011-2).
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Liang, C., Lan, Y., Sun, Z. et al. Synthesis of carbon quantum dots with iron and nitrogen from Passiflora edulis and their peroxidase-mimicking activity for colorimetric determination of uric acid. Microchim Acta 187, 405 (2020). https://doi.org/10.1007/s00604-020-04391-8
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DOI: https://doi.org/10.1007/s00604-020-04391-8