SiC-functionalized fluorescent aptasensor for determination of Proteus mirabilis

Abstract

Aptamer-modified SiC quantum dots (DNA-SiC QDs) as fluorescent aptasensor are described for the determination of Proteus mirabilis. The SiC QDs were synthesized through one-pot hydrothermal method with particle sizes of about 14 nm. The amino-modified aptamers against P. mirabilis were conjugated to the surfaces of SiC QDs for bacteria recognition. The aptamer with an affinity for target protein can bound to P. mirabilis and this causes a decrease in the fluorescence intensity of DNA-SiC QDs. P. mirabilis levels were tested by the aptasensor within 35 min with fluorescence excitation/emission maxima at 320/420 nm. The linear range is from 103 to 108 CFU mL−1 and the limit of detection is 526 CFU mL−1 (S/N = 3). The aptasensor was used for determination of P. mirabilis in pure milk samples and obtained good accuracy (87.6–104.5%) and recovery rates (85–110.2%) were obtained. The detection in simulated forensic identification samples (pure milk, milk powder, blood, and urine) obtained gave satisfactory coincidence rates with the method of bacterial isolation and identification as standard. These results demonstrate that the fluorescent aptasensor is a potential tool for identification of P. mirabilis in forensic food poisoning cases.

Determination of P. mirabilis is based on SiC QDs fluorescence aptasensor. The SiC QDs with plentiful carboxyl groups on the surface can be synthesized via one-pot hydrothermal route. After activated by EDC/NHS, the SiC QDs can bind to aptamer to form fluorescence aptasensors. When the target P. mirabilis exists, the fluorescence of aptasensor will be quenched and the determination of the P. mirabilis based on the fluorescence change can be analyzed.

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Funding

This study was supported by the National Natural Science Foundation of China (Nos. 81772025 and 81772026).

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Correspondence to Chensen Ding or Yanjun Ding.

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Yao, W., Shi, J., Ling, J. et al. SiC-functionalized fluorescent aptasensor for determination of Proteus mirabilis. Microchim Acta 187, 406 (2020). https://doi.org/10.1007/s00604-020-04378-5

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Keywords

  • SiC QD aptasensor
  • Foodborne pathogen
  • P. mirabilis determination
  • Forensic food poisoning cases