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Colorimetric determination of ascorbic acid using a polyallylamine-stabilized IrO2/graphene oxide nanozyme as a peroxidase mimic

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Abstract

The authors describe a peroxidase-mimicking nanozyme composed of IrO2 and graphene oxide (GO). It was synthesized from monodisperse IrO2 nanoparticles with an average diameter of 1.7 ± 0.3 nm that were prepared by pulsed laser ablation in ethanol. The nanoparticles were then placed on polyallylamine-modified GO nanosheets through electrostatic interaction. The peroxidase-like activity of the resulting nanocomposites was evaluated by catalytic oxidation of 3,3′,5,5′-tetramethylbenzidine in the presence of H2O2. Kinetic results demonstrated that the catalytic behavior of the nanocomposites follows Michaelis-Menten kinetics. Experiments performed with terephthalic acid and cytochrome C confirmed that the peroxidase-like activity originated from the electron transfer mechanism rather than from generation of hydroxy radicals. The peroxidase-like activity is inhibited in the presence of ascorbic acid (AA). Based on this property, a colorimetric assay was developed for the determination of AA by exploiting the peroxidase-like activity of IrO2/GO nanocomposites. The linear relationship between absorbance at 652 nm and the concentration of AA was acquired. The limit of detection for AA is 324 nM. Further applications of the method for AA detection in real samples were also successfully demonstrated.

Schematic of the preparation of polyallylamine (PAH)-stabilized IrO2/GO nanocomposites and the colorimetric detection of AA based on the peroxidase-like activity of IrO2/GO nanocomposites.

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Acknowledgments

This work was supported by National key research and development program from the Ministry of Science and Technology of China (2016YFC0207102) and National Natural Science Foundation of China (21573050).

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Author notes

  1. Huiyuan Sun and Xueliang Liu contributed equally to this work.

Authors and Affiliations

  1. CAS Key Lab for Biomedical Effects of Nanomaterials and Nanosafety, Center of Materials Science and Optoelectronics Engineering, CAS center for Excellence in Nanoscience, National Center for Nanoscience and Technology, UCAS, Beijing, 100190, People’s Republic of China

    Huiyuan Sun, Xueliang Liu, Xinhuan Wang, Qiusen Han, Cui Qi, Chen Wang & Rong Yang

  2. Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing, 100084, People’s Republic of China

    Huiyuan Sun, Yanmei Li & Yongxiang Chen

  3. Sino-Danish College, UCAS, Sino-Danish Center for Education and Research, Beijing, 100190, People’s Republic of China

    Qiusen Han, Chen Wang & Rong Yang

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  1. Huiyuan Sun
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Sun, H., Liu, X., Wang, X. et al. Colorimetric determination of ascorbic acid using a polyallylamine-stabilized IrO2/graphene oxide nanozyme as a peroxidase mimic. Microchim Acta 187, 110 (2020). https://doi.org/10.1007/s00604-019-3897-4

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