A new dual-emission ratiometric fluorescent probe was synthesized and successfully used for the determination of Malachite Green (MG) in fish farming water. The ratiometric fluorescent probe was successfully composited by sol-gel method using C3N4 and CdTe quantum dots as fluorescent materials combined with mesoporous molecularly imprinted polymers. MG quenches the red fluorescence of the CdTe QDs (with excitation/emission wavelengths at 350/680 nm) while the blue fluorescence of C3N4 (with excitation/emission wavelengths at 350/458 nm) remains unchanged. The change of fluorescence color and fluorescence intensity ratio can be successfully used for quantification of malachite green. In addition, the mesoporous structure has a large surface and good adsorption capacity for malachite green. The normalized intensity of fluorescence increases linearly in the 50–1000 ng·mL−1 MG concentration range, and the detection limit is 10 ng·mL−1. The imprinting factor is 3.2. The nanoprobe was applied to the determination of MG in fish farming water samples. Recoveries and relative standard deviations were 92.5–97.8% and 2.5–6.2%, respectively.
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This work was supported by the Fundamental Research Funds for the Central Universities (No.2572017 EB08) and was supported by Natural Science Foundation of Heilongjiang Province of China (No.JJ2018ZR0081).
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Shi, H., Zhang, L., Yu, G. et al. Molecularly imprinted mesoporous silica incorporating C3N4 dots and CdTe quantum dots as ratiometric fluorescent probe for determination of Malachite Green. Microchim Acta 186, 556 (2019). https://doi.org/10.1007/s00604-019-3670-8
- Dual emission
- Mesoporous structure
- Molecularly imprinted polymers
- Fish farming water