The feasibility of using carbosilane dendronized gold nanoparticles (GNPs) for protein sample preparation was evaluated. Three different dendrons with three different generations (1G, 2G, and 3G) were employed to modify the GNPs, viz. sulfonate terminated (STC-GNPs), carboxylate terminated (CTC-GNPs), and trimethylammonium terminated (ATC-GNPs) dendrons. The synthesis of the CTC-GNP is described. The other dendronized GNPs were synthesized using previously described routes. Bovine serum albumin, lysozyme, and myoglobin were employed to study the potential of GNPs to interact with proteins. The interaction between the GNPs and the proteins was evaluated using fluorescence spectroscopy and polyacrylamide gel electrophoresis. The CTC-GNPs and STC-GNPs under acidic and neutral conditions, respectively, promoted the establishment of electrostatic interactions with positively charged proteins. Proteins from 10 to 75 kDa molecular weights interacted with GNPs at protein: nanoparticle ratios of 1:0.25. The GNPs were applied to the extraction of proteins from a peach seed. In the authors’ perception, the method is a clean alternative to established extraction methods based on the use of organic or polluting chemicals.
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This work was supported by the Spanish Ministry of Economy and Competitiveness (ref. AGL2016-79010-R and CTQ2017-86224-P). R.V.-V thanks the University of Alcalá for the pre-doctoral contract. CIBER-BBN is an initiative funded by the VI National R&D&I Plan 2008-2011, Iniciativa Ingenio 2010, Consolider Program, CIBER Actions and financed by the Instituto de la Salud Carlos III with assistance from the European Regional Development Fund.
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Electronic Supplementary Material
Complete description of the preparation of first to third generation carboxylate terminated carbosilane dendrons. Figures of scheme of synthesis and structures of carboxylate dendrons, NMR spectra of dendrons. Gel corresponding to the protein that interacted 1G STC-GNP. Table of Mw, isoelectric point (pI), net charge at different pHs, and number of Trp residues for BSA, Myo, and Lyz. (DOCX 709 kb)
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Vásquez-Villanueva, R., Peña-González, C.E., Sánchez-Nieves, J. et al. Gold nanoparticles coated with carbosilane dendrons in protein sample preparation. Microchim Acta 186, 508 (2019). https://doi.org/10.1007/s00604-019-3587-2
- Protein extraction