Abstract
An electrochemical biosensor for microRNA was constructed on the basis of direct growth of electroactive Prussian Blue (PB) on graphene oxide (GO). A mercapto-modified probe DNA that is complementary to the hepatocellular carcinoma biomarker microRNA-122 was firstly anchored on a gold electrode (AuE). Then, GO (with its large surface and multiple active sites) was adsorbed on probe DNA through π-interaction. Subsequently, the PB nanoparticles were directly grown on GO via alternative dipping the electrode in solutions of FeCl3 and hexacyanoferrate(III). Upon incubation of the resulting electrode with a solution of microRNA-122, the probe DNA on the electrode interacts with microRNA-122 to form a rigid duplex. This results in the release of electroactive PB/GO from the sensing interface and a decrease in current, typically measured at 0.18 V (vs. Ag/AgCl (3 M KCl)). The sensor covers the 10 fM to 10 nM microRNA-122 concentration range and has a 1.5 fM detection limit. The method was successfully applied to the determination of microRNA-122 in real biological samples.
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Acknowledgements
This work was financially supported by the National Natural Science Foundation of China (Nos. 81873978, 81601842, 21802064), the Sixth Talent Peaks Project of Jiangsu Province (2018-WSW-068), the 64th grant of Chinese Post-doctoral Science Foundation (2018 M642298), and Foundation of Key Laboratory of Sensor Analysis of Tumor Marker from Ministry of Education, Qingdao University of Science and Technology.
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Wang, F., Chu, Y., Ai, Y. et al. Graphene oxide with in-situ grown Prussian Blue as an electrochemical probe for microRNA-122. Microchim Acta 186, 116 (2019). https://doi.org/10.1007/s00604-018-3204-9
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DOI: https://doi.org/10.1007/s00604-018-3204-9