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Simultaneous determination of dopamine and uric acid in the presence of ascorbic acid using a gold electrode modified with carboxylated graphene and silver nanocube functionalized polydopamine nanospheres

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Abstract

A voltammetric sensor is presented for the simultaneous determination of dopamine (DA) and uric acid (UA) in the presence of ascorbic acid (AA). It is based on a gold electrode (GE) modified with carboxyl-functionalized graphene (CFG) and silver nanocube functionalized DA nanospheres (AgNC@PDA-NS). The AgNC@PDA-NS nanocomposite was characterized by scanning electron microscopy and UV-Vis spectroscopy. The electrochemical behavior of the modified electrode was evaluated by electrochemical impedance spectroscopy, cyclic voltammetry and differential pulse voltammetry. The modified electrode displays good electrocatalytic activity towards DA (typically at 0.14 V vs. Ag/AgCl) and UA (typically at 0.29 V vs. Ag/AgCl) even in the presence of ascorbic acid. Response to DA is linear in the concentration range of 2.5 to 130 μM with a detection limit of 0.25 μM. Response to UA is linear in the concentration range of 10 to 130 μM with a detection limit of 1.9 μM. In addition, the sensitivity for DA and UA is 0.538 and 0.156 μA μM−1 cm−2, respectively. The modified electrode also displays good stability, selectivity and reproducibility.

The gold electrode modified with polydopamine nanospheres functionalized with silver nanocube and carboxylated graphene is used for simultaneous determination of DA and UA in the presence of AA, with wide linear range and low detection limit.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 21175115), the Natural Science Foundation of Fujian province in China (2016 J01067), and the Innovation Base Foundation for Graduate Students Education of Fujian Province.

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Authors and Affiliations

  1. College of Chemistry and Environment, Minnan Normal University, Zhangzhou, Fujian, 363000, People’s Republic of China

    Yancai Li, Yingying Jiang, Yingying Song, Yuhui Li & Shunxing Li

  2. Fujian Province Key Laboratory of Modern Analytical Science and Separation Technology, Minnan Normal University, Zhangzhou, Fujian, 363000, People’s Republic of China

    Yancai Li & Shunxing Li

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Li, Y., Jiang, Y., Song, Y. et al. Simultaneous determination of dopamine and uric acid in the presence of ascorbic acid using a gold electrode modified with carboxylated graphene and silver nanocube functionalized polydopamine nanospheres. Microchim Acta 185, 382 (2018). https://doi.org/10.1007/s00604-018-2922-3

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