Microchimica Acta

, 185:310 | Cite as

Dye sensitized photoelectrochemical immunosensor for the tumor marker CEA by using a flower-like 3D architecture prepared from graphene oxide and MoS2

Original Paper

Abstract

The authors describe a dye-sensitized photoelectrochemical immunoassay for the tumor marker carcinoembryonic antigen (CEA). The method employs the rhodamine dye Rh123 with red color and absorption maximum at 500 nm for spectral sensitization, and a 3D nanocomposite prepared from graphene oxide and MoS2 acting as the photoelectric conversion layer. The nanocomposite with flower-like 3D architectures was characterized by transmission electron microscopy, scanning electron microscopy, X-ray powder diffraction, and UV-vis diffuse reflectometry. A photoelectrochemical sandwich immunoassay was developed that is based on the use of the nanocomposite and based on the specific binding of antibody and antigen, and by using a secondary antibody labeled with Rh123 and CdS (Ab2-Rh123@CdS). Under optimal conditions and at a typical working voltage of 0 V (vs. Hg/HgCl2), the photocurrent increases linearly 10 pg mL−1 to 80 ng mL−1 CEA concentration range, with a 3.2 pg mL−1 detection limit.

Graphical abstract

Flower-like GO-MoS2 complex with high efficiency of electron transport was synthesized to construct photoelectrochemical platform. The sandwich-type immunoassay was built on this platform based on specific binding of antigen and antibody. Carcinoembryonic antigen in sample was detected sensitively by using sensitization of rhodamine dye Rh123 as signal amplification strategy.

Keywords

Immunoassay Sandwich-type Signal amplification Sensitization Two-dimension nanomaterials 

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (Grant No. 21605111 and 21706178), and Natural Science Foundation of Shanxi Province (No. 201601D021037 and 201601D011079).

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2018_2853_MOESM1_ESM.doc (2.7 mb)
ESM 1 (DOC 2748 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  • Kaijing Song
    • 1
  • Chuanmin Ding
    • 1
  • Bing Zhang
    • 1
  • Honghong Chang
    • 1
  • Zhihuan Zhao
    • 1
  • Wenlong Wei
    • 1
  • Junwen Wang
    • 1
  1. 1.College of Chemistry and Chemical EngineeringTaiyuan University of TechnologyTaiyuanChina

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