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Ionic liquid coated iron nanoparticles are promising peroxidase mimics for optical determination of H2O2

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Abstract

Ionic liquid coated nanoparticles (IL-NPs) consisting of zero-valent iron are shown to display intrinsic peroxidase-like activity with enhanced potential to catalyze the oxidation of the chromogenic substrate 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of hydrogen peroxide. This results in the formation of a blue green colored product that can be detected with bare eyes and quantified by photometry at 652 nm. The IL-NPs were further doped with bismuth to enhance its catalytic properties. The Bi-doped IL-NPs were characterized by FTIR, X-ray diffraction and scanning electron microscopy. A colorimetric assay was worked out for hydrogen peroxide that is simple, sensitive and selective. Response is linear in the 30–300 μM H2O2 concentration range, and the detection limit is 0.15 μM.

Schematic of ionic liquid coated iron nanoparticles that display intrinsic peroxidase-like activity. They are capable of oxidizing the chromogenic substrate 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of hydrogen peroxide. This catalytic oxidation generated blue-green color can be measured by colorimetry. Response is linear in the range of 30–300 μM H2O2 concentration, and the detection limit is 0.15 μM.

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Acknowledgements

This work has been supported by IRCBM, COMSATS Institute of Information Technology Lahore, Pakistan.

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Authors and Affiliations

  1. Department of Chemistry, Government College University, Lahore, 54000, Pakistan

    Faiza Zarif & Muhammad Zahid Qureshi

  2. Interdisciplinary Research Centre in Biomedical Materials (IRCBM) COMSATS Institute of Information Technology, Lahore, 54000, Pakistan

    Sajid Rauf, Akhtar Hayat, Nawshad Muhammad, Abdur Rahim, Mian Hasnain Nawaz & Muhammad Nasir

  3. Department of Environmental Sciences, COMSATS Institute of Information Technology, Vehari, 61100, Pakistan

    Noor Samad Shah

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  1. Faiza Zarif
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Correspondence to Akhtar Hayat or Nawshad Muhammad.

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Zarif, F., Rauf, S., Qureshi, M.Z. et al. Ionic liquid coated iron nanoparticles are promising peroxidase mimics for optical determination of H2O2. Microchim Acta 185, 302 (2018). https://doi.org/10.1007/s00604-018-2841-3

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