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Microchimica Acta

, 185:309 | Cite as

Magnetic microspheres modified with Ti(IV) and Nb(V) for enrichment of phosphopeptides

  • Jiebing Jiang
  • Xueni Sun
  • Xiaojian She
  • Jiajia Li
  • Yan Li
  • Chunhui Deng
  • Gengli Duan
Original Paper
  • 109 Downloads

Abstract

Magnetic microspheres (Fe3O4) were coated with polydopamine (PDA) and loaded with the metal ions Ti(IV) and Nb(V) to give a material of type Fe3O4@PDA-Ti/Nb. It is shown to be useful for affinity chromatography and for enrichment of phosphopeptides from both standard protein solutions and real samples. For comparison, such microspheres loaded with single metal ions only (Fe3O4@PDA-Ti and Fe3O4@PDA-Nb) and their physical mixtures were also investigated under identical conditions. The binary metal ion-loaded magnetic microspheres display better enrichment efficiency than the single metal ion-loaded microspheres and their physical mixture. Both multiphosphopeptides and monophosphopeptides can be extracted. The Fe3O4@PDA-Ti/Nb microspheres exhibit ultra-high sensitivity (the lowest detection amount being 2 fmol) and selectivity at a low mass ratio such as in case of β-casein/BSA (1:1000).

Graphical abstract

Magnetic microspheres (Fe3O4) were coated with polydopamine (PDA) and loaded with the metal ions Ti(IV) and Nb(V) to give a material of type Fe3O4@PDA-Ti/Nb. Results showed its great potential as an affinity probe in phosphoproteome research due to rapid magnetic separation of phosphopeptides, ultrahigh sensitivity and selectivity, and remarkable reusability.

Keywords

Metal ions combination Immobilized metal ion affinity chromatography MALDI-TOF mass spectrometry Polydopamine Affinity probe 

Notes

Acknowledgements

This work was supported by funds provided by the Natural Science Foundation of China (Project no. 21675034), the Natural Science Foundation of Shanghai (Project no. 16ZR1402300), the Outstanding Talent Plan of Fudan University (Project no. JJF301038) and the Ministry of Science and Technology of the People’s Republic of China (Grant no. 2018ZX09J18112).

Compliance with ethical standards

The authors declare that they have no competing interests.

Supplementary material

604_2018_2837_MOESM1_ESM.doc (4.5 mb)
ESM 1 (DOC 4654 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  • Jiebing Jiang
    • 1
  • Xueni Sun
    • 2
  • Xiaojian She
    • 1
  • Jiajia Li
    • 1
  • Yan Li
    • 1
  • Chunhui Deng
    • 3
  • Gengli Duan
    • 1
  1. 1.Fudan University Affiliated Pudong Medical Center & Pharmaceutical Analysis Department, School of PharmacyFudan UniversityShanghaiChina
  2. 2.Institute of Functional GenomicsUniversity of RegensburgRegensburgGermany
  3. 3.Department of Chemistry and Institutes of Biomedical SciencesFudan UniversityShanghaiChina

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