Microchimica Acta

, 185:295 | Cite as

A microfluidic chip containing a molecularly imprinted polymer and a DNA aptamer for voltammetric determination of carbofuran

  • Shuhuai Li
  • Jianping Li
  • Jinhui Luo
  • Zhi Xu
  • Xionghui Ma
Original Paper


An electrochemical microfluidic chip is described for the determination of the insecticide carbofuran. It is making use of a molecularly imprinted film (MIP) and a DNA aptamer as dual recognition units. The analyte (carbofuran) is transported to the MIP and captured at the identification site in the channel. Then, carbofuran is eluted with carbinol-acetic acid and transported to the DNA aptamer on the testing position of the chip. It is captured again, this time by the aptamer, and detected by differential pulse voltammetry (DPV). The dual recognition (by aptamer and MIP) results in outstanding selectivity. Additionally, graphene oxide-supported gold nanoparticles (GO-AuNPs) were used to improve the sensitivity of electrochemical detector. DPV response is linear in the 0.2 to 50 nM carbofuran concentration range at a potential of −1.2 V, with a 67 pM detection limit. The method has attractive features such as its potential for high throughput, high degree of automation, and high integration. Conceivably, the method may be extended to other analytes for which appropriate MIPs and aptamers are available.

Graphical abstract

Schematic of an electrochemical microfluidic chip for carbofuran detection based on a molecularly imprinted film (MIP) and a DNA aptamer as dual recognition units. In the chip, targets were recognized by MIP and aptamer, respectively. It shows promising potential for the design of electrochemical devices with high throughput, high automation, and high integration.


X-ray photoelectron spectroscopy Transmission electron microscopy X-ray diffraction Fourier transform infrared spectra Differential pulse voltammetry Ultraviolet–visible absorption spectroscopy Polydimethylsiloxane Graphene oxide Gold nanoparticles Pesticide residues 



This project was supported by the Natural Science Foundation of Hainan Province of China (No. 217222) and the Central Public-interest Scientific Institution Basal Research Fund for Chinese Academy of Tropical Agricultural Sciences (No. 1630082017002 and 1630082018003).

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2018_2835_MOESM1_ESM.doc (675 kb)
ESM 1 (DOC 674 kb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Analysis and Test Center of Chinese Academy of Tropical Agricultural SciencesHaikouChina
  2. 2.Hainan Provincial Key Laboratory of Quality and Safety for Tropical Fruits and VegetablesHaikouChina
  3. 3.College of Chemistry and BioengineeringGuilinUniversity of TechnologyGuilinChina

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