Advertisement

Microchimica Acta

, 185:301 | Cite as

Colorimetric detection of DNA at the nanomolar level based on enzyme-induced gold nanoparticle de-aggregation

Original Paper

Abstract

The authors describe a colorimetric method for the determination of DNA based on the deaggregation of gold nanoparticles (AuNPs) induced by exonuclease III (Exo III). DNA amplification is accomplished by Exo III to generate large quantities of the residual DNA. Residual DNA tethers onto the surfaces of AuNPs which prevents their aggregation. Hence, the color of the solution is red. However, in the absence of DNA, salt-induced aggregation is not prevented, and the bluish-purple color of the aggregated AuNPs is observed. The ratio of absorbances at 525 and 625 nm increases up to 150 nM DNA concentrations, and the LOD is as low as 3.0 nM. It is shown that the presence of 300 nM concentrations of random DNA (with a mass up to 10-fold that of target DNA) does not interfere. The method was successfully applied to the analysis of DNA in spiked serum samples. The method is simple, reliable, and does not require complicated amplification steps and expensive instrumentation.

Graphical abstract

Schematic of a sensing strategy for DNA detection by exonuclease III-induced deaggregation of gold nanoparticles. DNA concentrations as  low as 3 nM can be detected via colorimetric monitoring of the color change from red to purple-blue.

Keywords

Exonuclease III Nucleic acid detection Colorimetric assay DNA recycling Amplification Absorbance Serum samples 

Notes

Acknowledgements

All authors gratefully acknowledge the financial support of Scientific Research Project of Beijing Educational Committee (Grant No. KM201710028009).

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2018_2833_MOESM1_ESM.doc (1.3 mb)
ESM 1 (DOC 1337 kb)

References

  1. 1.
    Lu W, Yuan Q, Yang Z, Yao B (2016) Self-primed isothermal amplification for genomic DNA detection of human papillomavirus. Biosens Bioelectron 90:258–263CrossRefGoogle Scholar
  2. 2.
    Park BH, Oh SJ, Jung JH, Choi G, Seo JH, Kim DH, Lee EY, Seo TS (2017) An integrated rotary microfluidic system with DNA extraction, loop-mediated isothermal amplification, and lateral flow strip based detection for point-of-care pathogen diagnostics. Biosens Bioelectron 91:334–340CrossRefGoogle Scholar
  3. 3.
    Zhou Q, Zheng J, Qing Z, Zheng M, Yang J, Yang S, Ying L, Yang R (2016) Detection of circulating tumor DNA in human blood via DNA-mediated surface enhanced Raman spectroscopy of single-walled carbon nanotubes. Anal Chem 88(9):4759–4765CrossRefGoogle Scholar
  4. 4.
    Ji H, Yan F, Lei J, Ju H (2012) Ultrasensitive electrochemical detection of nucleic acids by template enhanced hybridization followed with rolling circle amplification. Anal Chem 84:7166–7171CrossRefGoogle Scholar
  5. 5.
    Vardevanyan PO, Antonyan AP, Parsadanyan MA, Davtyan HG, Karapetyan AT (2003) The binding of ethidium bromide with DNA: interaction with single- and double-stranded structures. Exp Mol Med 35:527–533CrossRefGoogle Scholar
  6. 6.
    Zhao B, Yan J, Wang D, Ge Z, He S, He D, Song S, Fan C (2013) Carbon nanotubes multifunctionalized by rolling circle amplification and their application for highly sensitive detection of cancer markers. Small 9:2595–2601CrossRefGoogle Scholar
  7. 7.
    Cheng Y, Zhang X, Li Z, Jiao X, Wang Y, Zhang Y (2009) Highly sensitive determination of microRNA using target-primed and branched rolling-circle amplification. Angew Chem Int Ed 48:3268–3272CrossRefGoogle Scholar
  8. 8.
    Mi L, Wen Y, Pan D, Wang Y, Fan C, Hu J (2009) Modulation of DNA polymerases with gold nanoparticles and their applications in hot-start PCR. Small 5:2597–2600CrossRefGoogle Scholar
  9. 9.
    Pollet J, Janssen KP, Knez K, Lammertyn J (2011) Real-time monitoring of solid-phase PCR using fiber-optic SPR. Small 7:1003–1006CrossRefGoogle Scholar
  10. 10.
    Hsieh K, Patterson AS, Ferguson BS, Plaxco KW, Soh HT (2012) Rapid, sensitive, and quantitative detection of pathogenic DNA at the point of care via microfluidic electrochemical quantitative loop-mediated isothermal amplification (MEQ-LAMP). Angew Chem Int Ed 51:4896–4900CrossRefGoogle Scholar
  11. 11.
    Wong JK, Yip SP, Lee TM (2014) Ultrasensitive and closed-tube colorimetric loop-mediated isothermal amplification assay using carboxyl-modified gold nanoparticles. Small 10:1495–1499CrossRefGoogle Scholar
  12. 12.
    Xue Q, Lv Y, Zhang Y, Xu S, Li R, Yue Q, Li H, Wang L, Gu X, Zhang S, Liu J (2014) Ultrasensitive fluorescence detection of nucleic acids using exonuclease III-induced cascade two-stage isothermal amplification-mediated zinc (II)-protoporphyrin IX/G-quadruplex supramolecular fluorescent nanotags. Biosens Bioelectron 61:351–356CrossRefGoogle Scholar
  13. 13.
    Li JJ, Liu QY, Xi HY, Wei XC, Chen ZB (2017) Y-shaped DNA duplex structure-triggered gold nanoparticle dimers for ultrasensitive colorimetric detection of nucleic acid with the dark-field microscope. 2017, 89: 12850–12856Google Scholar
  14. 14.
    Yang LM, Liu B, Li N, Tang B (2017) Fluorescent nanoprobe for detection and imaging of nucleic acid molecules. Acta Chim Sin 75:1047–1060CrossRefGoogle Scholar
  15. 15.
    Rasheed PA, Sandhyarani N (2017) Electrochemical DNA sensors based on the use of gold nanoparticles: a review on recent developments. Microchim Acta 184:981–1000CrossRefGoogle Scholar
  16. 16.
    Liu QY, Yang YT, Li H, Zhu RR, Shao Q, Yang SG, Xu JJ (2015) NiO nanoparticles modified with 5,10,15,20-tetrakis(4-carboxyl pheyl)-porphyrin: promising peroxidase mimetics for H2O2 and glucose detection. Biosens Bioelectron 64:147–153CrossRefGoogle Scholar
  17. 17.
    Zhang LY, Chen MX, Jiang YL, Chen MM, Ding YN, Liu QY (2017) A facile preparation of montmorillonite-supported copper sulfide nanocomposites and their application in the detection of H2O2. Sensors Actuators B Chem 239:28–35CrossRefGoogle Scholar
  18. 18.
    Sun LF, Ding YY, Jiang YL, Liu QY (2017) Montmorillonite-loaded ceria nanocomposites with superior peroxidase-like activity for rapid colorimetric detection of H2O2. Sensors Actuators B Chem 239:848–856CrossRefGoogle Scholar
  19. 19.
    Ding YN, Yang BC, Liu H, Liu ZX, Zhang X, Zheng XW, Liu QY (2018) FePt-au ternary metallic nanoparticles with the enhanced peroxidase-like activity for ultrafast colorimetric detection of H2O2. Sensors Actuators B Chem 259:775–783CrossRefGoogle Scholar
  20. 20.
    Liu QY, Yang YT, Lv XT, Ding YN, Zhang YZ, Jing JJ, Xu CX (2017) One-step synthesis of uniform nanoparticles of porphyrin functionalized ceria with promising peroxidase mimetics for H2O2 and glucose colorimetric detection. Sensors Actuators B Chem 240:726–734CrossRefGoogle Scholar
  21. 21.
    Liu QY, Chen PP, Xu Z, Chen MM, Ding YN, Yue K, Xu J (2017) A facile strategy to prepare porphyrin functionalized ZnS nanoparticles and their peroxidase-like catalytic activity for colorimetric sensor of hydrogen peroxide and glucose. Sensors Actuators B Chem 251:339–348CrossRefGoogle Scholar
  22. 22.
    Chen MM, Sun LF, Ding YN, Shi ZQ, Liu QY (2017) N,N’-di-caboxymethyl perylene diimides functionalized magnetic nanocomposites with enhanced peroxidase-like activity for colorimetric sensor of H2O2 and glucose. New J Chem 41:5853–5862CrossRefGoogle Scholar
  23. 23.
    Gao FL, Du Y, Yao JW, Zhang YZ, Gao J (2015) A novel electrochemical biosensor for DNA detection based on exonuclease III-assisted target recycling and rolling circle amplification. RSC Adv 5:9123–9129CrossRefGoogle Scholar
  24. 24.
    Gao FL, Lei JP, Ju HX (2013) Ultrasensitive fluorescence detection of bleomycin via exonuclease III-aided DNA recycling amplification. Chem Commun 49:7561–7563CrossRefGoogle Scholar
  25. 25.
    Mirkin CA, Letsinger RL, Mucic RC, Storhoff JJ (1996) A DNA-based method for rationally assembling nanoparticles into macroscopic materials. Nature 382:607–609CrossRefGoogle Scholar
  26. 26.
    Jung YK, Park HG (2015) Colorimetric detection of clinical DNA samples using an intercalator- conjugated polydiacetylene sensor. Biosens Bioelectron 72:127–132CrossRefGoogle Scholar
  27. 27.
    Zhang P, Zhang CS, Shu BW (2016) Micropatterned paper devices using amine-terminatedpolydiacetylene vesicles as colorimetric probes for enhanced detection of double-stranded DNA. Sensors Actuators B Chem 236:27–34CrossRefGoogle Scholar
  28. 28.
    Kim MI, Park KS, Park HG (2014) Ultrafast colorimetric detection of nucleic acids based on the inhibition of the oxidase activity of cerium oxide nanoparticles. Chem Comm 50:9577–9580CrossRefGoogle Scholar
  29. 29.
    Yan JW, Tian YG, Tan JH, Huang ZS (2015) Colorimetric and fluorescence detection of G-quadruplex nucleic acids with a coumarin-benzothiazole probe. Analyst 140:7146–7149CrossRefGoogle Scholar
  30. 30.
    Chau LY, He QJ, Qin AL, Yip SP, Lee TMH (2016) Platinum nanoparticles on reduced graphene oxide as peroxidase mimetics for the colorimetric detection of specific DNA sequence. J Mater Chem B 4:4076–4083CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  1. 1.College of Chemistry and Chemical EngineeringXinxiang UniversityXinxiangChina
  2. 2.Department of ChemistryCapital Normal UniversityBeijingChina

Personalised recommendations