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Colorimetric detection of DNA at the nanomolar level based on enzyme-induced gold nanoparticle de-aggregation

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Abstract

The authors describe a colorimetric method for the determination of DNA based on the deaggregation of gold nanoparticles (AuNPs) induced by exonuclease III (Exo III). DNA amplification is accomplished by Exo III to generate large quantities of the residual DNA. Residual DNA tethers onto the surfaces of AuNPs which prevents their aggregation. Hence, the color of the solution is red. However, in the absence of DNA, salt-induced aggregation is not prevented, and the bluish-purple color of the aggregated AuNPs is observed. The ratio of absorbances at 525 and 625 nm increases up to 150 nM DNA concentrations, and the LOD is as low as 3.0 nM. It is shown that the presence of 300 nM concentrations of random DNA (with a mass up to 10-fold that of target DNA) does not interfere. The method was successfully applied to the analysis of DNA in spiked serum samples. The method is simple, reliable, and does not require complicated amplification steps and expensive instrumentation.

Schematic of a sensing strategy for DNA detection by exonuclease III-induced deaggregation of gold nanoparticles. DNA concentrations as  low as 3 nM can be detected via colorimetric monitoring of the color change from red to purple-blue.

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Acknowledgements

All authors gratefully acknowledge the financial support of Scientific Research Project of Beijing Educational Committee (Grant No. KM201710028009).

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Authors and Affiliations

  1. College of Chemistry and Chemical Engineering, Xinxiang University, Xinxiang, 453003, China

    Qingling Liu & Li Li

  2. Department of Chemistry, Capital Normal University, Beijing, 100048, China

    Yan Zhao & Zhengbo Chen

Authors
  1. Qingling Liu
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  2. Li Li
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  3. Yan Zhao
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  4. Zhengbo Chen
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Correspondence to Li Li or Zhengbo Chen.

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Liu, Q., Li, L., Zhao, Y. et al. Colorimetric detection of DNA at the nanomolar level based on enzyme-induced gold nanoparticle de-aggregation. Microchim Acta 185, 301 (2018). https://doi.org/10.1007/s00604-018-2833-3

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  • DOI: https://doi.org/10.1007/s00604-018-2833-3

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