Amperometric determination of ascorbic acid with a glassy carbon electrode modified with TiO2-gold nanoparticles integrated into carbon nanotubes
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A glassy carbon electrode was modified with a TiO2-gold nanoparticle hybrid integrated with multi-walled carbon nanotubes in a dihexadecylphosphate film (TiO2-Au NP-MWCNT-DHP/GCE) and applied to amperometric determination of ascorbic acid (AA). The modified sensor displays fast charge transfer and shows an irreversible anodic behavior for AA by cyclic voltammetry. Under optimal experimental conditions and using amperometry at 0.4 V, the analytical curve presented a statistical linear concentration range for AA from 5.0 to 51 μmol L−1, with a limit of detection of 1.2 μmol L−1. The electrode was successfully applied to the determination of AA in pharmaceutical and fruit juice without the need for major pretreatment of samples.
KeywordsElectrochemical sensing Modified electrode Nanostructured material Hybrid material Pharmaceuticals
The authors gratefully acknowledge financial support and scholarships from the funding agencies Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP; Grant numbers 2015/21366-9 and 2015/11452-5) and Fundação Araucária do Paraná. Special thanks to Juliane Cristina Leme for her kind help in statistical analyses.
Compliance with ethical standards
The authors declare that they have no competing interests.
- 2.Varvara M, Bozzo G, Disanto C, et al (2016) The use of the ascorbic acid as food additive and technical-legal issues. Ital J Food Saf 5. https://doi.org/10.4081/ijfs.2016.4313
- 4.Ngai KS, Tan WT, Zainal Z et al (2013) Voltammetry detection of ascorbic acid at glassy carbon electrode modified by single-walled carbon nanotube/zinc oxide. Int J Electrochem Sci 8:10557–10567Google Scholar
- 5.Fernandes DM, Silva N, Pereira C, Moura C, Magalhães JMCS, Bachiller-Baeza B, Rodríguez-Ramos I, Guerrero-Ruiz A, Delerue-Matos C, Freire C (2015) MnFe2O4@CNT-N as novel electrochemical nanosensor for determination of caffeine, acetaminophen and ascorbic acid. Sensors Actuators B Chem 218:128–136. https://doi.org/10.1016/j.snb.2015.05.003 CrossRefGoogle Scholar
- 8.Aneesh PK, Nambiar SR, Rao TP, Ajayaghosh A (2014) Electrochemically synthesized partially reduced graphene oxide modified glassy carbon electrode for individual and simultaneous voltammetric determination of ascorbic acid, dopamine and uric acid. Anal Methods 6:5322–5330. https://doi.org/10.1039/c4ay00043a CrossRefGoogle Scholar
- 16.Matthews FL, Rawlings RD (2008) Composite materials: engineering and science. CRC PressGoogle Scholar
- 25.British Pharmacopoeia Commission (2013) British pharmacopoeia, 5th ed. The Stationery Office, LondonGoogle Scholar
- 26.Sartori ER, Vicentini FC, Fatibello-Filho O (2011) Indirect determination of sulfite using a polyphenol oxidase biosensor based on a glassy carbon electrode modified with multi-walled carbon nanotubes and gold nanoparticles within a poly(allylamine hydrochloride) film. Talanta 87:235–242. https://doi.org/10.1016/j.talanta.2011.10.003 CrossRefPubMedGoogle Scholar
- 27.Ardila JA, Oliveira GG, Medeiros RA, Fatibello-Filho O (2013) Determination of gemfibrozil in pharmaceutical and urine samples by square-wave adsorptive stripping voltammetry using a glassy carbon electrode modified with multi-walled carbon nanotubes within a dihexadecyl hydrogen phosphate film. J Electroanal Chem 690:32–37. https://doi.org/10.1016/J.JELECHEM.2012.11.038 CrossRefGoogle Scholar
- 30.Suresh S, Gupta M, Kumar GA, Rao VK, Kumar O, Ghosal P (2012) Synergic effect of multi-walled carbon nanotubes and gold nanoparticles towards immunosensing of ricin with carbon nanotube–gold nanoparticles–chitosan modified screen printed electrode. Analyst 137:4086–4092. https://doi.org/10.1039/c2an35279f CrossRefPubMedGoogle Scholar
- 31.Gosser DK (1993) Cyclic voltammetry: simulation and analysis of reaction mechanisms. John Wiley & Sons, Inc., New YorkGoogle Scholar
- 32.Bard AJ, Faulkner LR (2001) Electrochemical methods : fundamentals and applications, 2nd edn. John Wiley & Sons, New YorkGoogle Scholar
- 34.Yilmaz S, Sadikoglu M, Saglikoglu G et al (2008) Determination of ascorbic acid in tablet dosage forms and some fruit juices by DPV. Int J Electrochem Sci 3:1534–1542Google Scholar
- 35.Miller JM, Miller JC (2010) Statistics and Chemometrics for analytical chemistry, 6th edn. Pearson, HarlowGoogle Scholar