Fluorometric determination of dopamine by using molybdenum disulfide quantum dots
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A method is described for the rapid fluorometric determination of dopamine (DA) by using molybdenum disulfide quantum dots (MoS2 QDs) that were fabricated via an ammonium hydroxide etching method. The probe has a fluorescence (with excitation/emission peaks at 267/380 nm) that is quenched by DA with high selectivity over various interferences. This is attributed to a reaction that occurs between DA and the molybdate ions in pH 9 solutions of MoS2 QDs. The formation of organic molybdate complexes and of dopamine-quinone results in strong quenching of the fluorescence of the QDs which is due to both electron transfer and an inner filter effect. Under the optimum conditions, the assay works in the 0.1–100 μM DA concentration range, with two linear ranges and a 10 nM detection limit. The method was applied to the determination of DA in spiked artificial urine samples, where it gave recoveries ranging from 97.6 to 102.2%, demonstrating that the method a promising tool for rapid and selective detection of DA.
KeywordsDopamine Dynamic quenching Etching synthesis Fluorescent probe Transition metal dichalcogenides
This research was financed by the National Natural Science Foundation of China (21675127), the Fundamental Research Funds for the Northwest A&F University of China (2014YB093, 2452015257) and the Development Project of Qinghai Key Laboratory (2017-ZJ-Y10).
Compliance with ethical standards
The author(s) declare that they have no competing interests.
- 3.De Benedetto GE, Fico D, Pennetta A, Malitesta C, Nicolardi G, Lofrumento DD, De Nuccio F, La Pesa V (2014) A rapid and simple method for the determination of 3,4-dihydroxyphenylacetic acid, norepinephrine, dopamine, and serotonin in mouse brain homogenate by HPLC with fluorimetric detection. J Pharm Biomed Anal 98:266–270CrossRefGoogle Scholar
- 13.Zhang W, Shi S, Wang Y, Yu S, Zhu W, Zhang X, Zhang D, Yang B, Wang X, Wang J (2016) Versatile molybdenum disulfide based antibacterial composites for in vitro enhanced sterilization and in vivo focal infection therapy. Nano 8(22):11642–11648Google Scholar
- 22.Yin Z, Zhang X, Cai Y, Chen J, Wong JI, Tay Y-Y, Chai J, Wu J, Zeng Z, Zheng B, Yang HY, Zhang H (2014) Preparation of MoS2-MoO3 hybrid nanomaterials for light-emitting diodes. Angew Chem Int Ed 53(46):12560–12565Google Scholar
- 24.Zhang W, Wang Y, Zhang D, Yu S, Zhu W, Wang J, Zheng F, Wang S, Wang J (2015) A one-step approach to the large-scale synthesis of functionalized MoS2 nanosheets by ionic liquid assisted grinding. Nano 7(22):10210–10217Google Scholar
- 25.Li BL, Chen LX, Zou HL, Lei JL, Luo HQ, Li NB (2014) Electrochemically induced Fenton reaction of few-layer MoS2 nanosheets: preparation of luminescent quantum dots via a transition of nanoporous morphology. Nano 6(16):9831–9838Google Scholar
- 33.Liao X, Lang H, Xu W, Shen Y (2003) Determination of polyphenols in tea by ammonium molybdate spectrophotometry. Chin J Anal Lab 05:70–72 (in Chinese)Google Scholar