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A voltammetric immunosensor for clenbuterol based on the use of a MoS2-AuPt nanocomposite

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Abstract

An ultrasensitive immunosensor for the direct detection of the illegally used livestock feed clebuterol (CLB) is described. It is based on the use of a glassy carbon electrode modified with an MoS2-AuPt nanocomposite and on biotin-streptavidin interaction. The use of MoS2-AuPt accelerates electron transfer, and this leads to a sharp increase in the electrochemical signal for the electrochemical probe hydrogen peroxide. Differential pulse voltammetry was used to record the current signal at a peak potential of −0.18 V (vs SCE). Under optimal conditions, the electrode has a linear response in the 10 pg·mL−1 to 100 ng·mL−1 CLB concentration range and a 6.9 pg·mL−1 detection limit (based on the 3σ criterium). This immunosensor is sensitive, highly specific and acceptably reproducible, and thus represents a valuable tool for the determination of CLB in pork.

Schematic of a voltammetric immunosensor for the determination of clenbuterol (CLB) based on the use of a nanocomposite prepared from molybdenum disulfide and a gold-platinum alloy (MoS2-AuPt), and making use of the biotin-streptavidin system.

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Acknowledgements

This research was supported by the National Natural Science Foundation of China (Grant Nos. 31071093, 31170129, and 31200064) and the Science and Technology Planning Project of Yuzhong District of Chongqing City, China (No. 20140119).

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Author notes

  1. Renyue Ji and Shuai Chen contributed equally to this work.

Authors and Affiliations

  1. School of Public Health and Management, Chongqing Medical University, Box 197#, No.1 ,Yi Xue Yuan Road, Yuzhong district, Chongqing, 400016, People’s Republic of China

    Renyue Ji, Shuai Chen, Wei Xu, Zhen Qin, Jing Fu Qiu & Chao Rui Li

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  1. Renyue Ji
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  2. Shuai Chen
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  3. Wei Xu
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Correspondence to Jing Fu Qiu or Chao Rui Li.

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Ji, R., Chen, S., Xu, W. et al. A voltammetric immunosensor for clenbuterol based on the use of a MoS2-AuPt nanocomposite. Microchim Acta 185, 209 (2018). https://doi.org/10.1007/s00604-018-2746-1

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