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Aerobic training improves platelet function in type 2 diabetic patients: role of microRNA-130a and GPIIb

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Abstract

Aims

MicroRNAs (miRs) that are mediators of gene expression have been implicated in type 2 diabetes mellitus (T2DM). Platelet hyper-reactivity is one of the most important disorders in T2DM patients. In this study, we explored the effects of aerobic training (AT) on platelet aggregation and Glycoprotein IIb (GPIIb) receptor and miR-130a expression.

Methods

In a quasi-experimental controlled trial, 24 sedentary, eligible female participants with T2DM were selected (age 61.92 ± 3.63) and divided into AT and control (CON) groups based on their peak oxygen consumption (VO2peak). AT protocol was performed three times per week in non-consecutive days on a treadmill with mean intensity (60–75% VO2peak) for 8 weeks, while the control group refrained from any type of exercise training. Two blood samples were taken before and after this period. Real-time PCR was used to determine the expression of platelet GPIIb and miR-130a. Moreover, platelet indices (PLT, MPV, PDW, and PCT), collagen-induced platelet aggregation and glycemic variables were measured.

Results

Analyses of data showed that anthropometric variables, VO2peak and glycemic control improved significantly (P < 0.01) after AT. Furthermore, MPV, PDW (P < 0.01), and platelet aggregation (P < 0.001) decreased significantly following AT compared with control group. Platelet GPIIb expression down-regulated significantly (P < 0.05) in AT group but up-regulation of miR-130a expression was not significant between two groups (P > 0.05).

Conclusions

Platelet hyper-reactivity in T2DM females might be decreased not only by glycemic control and amelioration of anthropometric and platelet indices, but also the down-regulation of GPIIb following AT. However, more research is needed to determine the effects of exercise training on platelet miR-130a.

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Acknowledgements

The authors have no conflicts of interest. The authors would also like to thank the study participants for their cooperation and dedication. We wish to thank from University of Isfahan (Isfahan, Iran) and Cell-Based Therapies Research Center, Digestive Disease Research Institute (Tehran, Iran) for their financial support.

Funding

This research received no specific grant.

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Authors and Affiliations

  1. Department of Exercise Physiology, Faculty of Sport Sciences, University of Isfahan, Isfahan, Iran

    Atousa Akbarinia & Mehdi Kargarfard

  2. Cell-Based Therapies Research Center, Digestive Disease Research Institute, Tehran University of Medical Sciences, Tehran, Iran

    Mahmood Naderi

Authors
  1. Atousa Akbarinia
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  2. Mehdi Kargarfard
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  3. Mahmood Naderi
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Correspondence to Mehdi Kargarfard.

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Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Informed consent was obtained from all individual participants included in the study.

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Managed by Massimo Porta.

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Akbarinia, A., Kargarfard, M. & Naderi, M. Aerobic training improves platelet function in type 2 diabetic patients: role of microRNA-130a and GPIIb. Acta Diabetol 55, 893–899 (2018). https://doi.org/10.1007/s00592-018-1167-2

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  • DOI: https://doi.org/10.1007/s00592-018-1167-2

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