The diagnostic value of circulating microRNA-499 versus high-sensitivity cardiac troponin T in early diagnosis of ST segment elevation myocardial infarction

Abstract

Background

Ischemic heart disease is the single most common cause of death worldwide. In order to increase the accuracy of the diagnosis, new diagnostic methods have been introduced, including miRNA-499, which is a promising biomarkers in coronary atherosclerotic diseases (CAD), and acute myocardial infarction (AMI).

Aim of the work

To evaluate the diagnostic value of circulating miRNA-499 versus high-sensitivity cardiac troponin in early diagnosis of AMI with ST segment elevation within the first 6 h of complaint.

Subjects and methods

This study was conducted on 100 Egyptian patients who presented during the first 3 h of AMI in the emergency department and 98 healthy volunteers as a control group. Serum samples for both groups were analyzed using reverse transcription and real-time PCR by TaqMan® MicroRNA assays.

Results

The concentration of miRNA-499 was significantly elevated in STEMI patients compared to healthy controls (p < 0.001). Moreover, receiver operating characteristic (ROC) curve analysis showed that area under the curve (AUC) for miRNA-499 was 0.90 (95% CI, 0.845–0.955) with 89% sensitivity and 83% specificity, at which positive predictive value (PPV) was 94% andnegative predictive value (NPV) was 69%. Furthermore, miRNA-499 displayed significant positive correlation with hs-cTnT and higher expression than hs-cTnT in the first 3 h of chest pain in emergency department.

Conclusion

miRNA-499 is a promising reliable biomarker for early detection of STEMI patients.

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Fig. 1

Data availability

The datasets used and analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgments

All authors are very grateful to the patients and the volunteer control individuals who participated in this study, and also to all members of Cardiology Department and Clinical and Chemical Pathology Department, Cairo University, and particularly the staff members of catheterization laboratory for their help in completing this work.

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Affiliations

Authors

Contributions

DMA did the practical work, collected, analyzed and interpreted the patient data and was a major contributor in writing the manuscript. EFA revised the data set. YSK was responsible for sample collection according to the inclusion criteria, and sample storage according to the methodology protocol. HST revised the data set and designed the antiviral treatment protocol. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Doaa M. Abdou.

Ethics declarations

Ethics approval and consent to participate

Approval was obtained from Cardiology Department Council of Cairo University. All procedures performed in the study were under the ethical standards of the Kasr Al-Ainy School of Medicine and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. All the procedures were approved by the ethical committee of the Kasr Al-Ainy School of Medicine, and written informed consent was obtained from each participant after a full explanation of the study protocol. All procedures, including individual data, were treated with confidentiality.

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Not applicable.

Competing interests

The authors declare no competing interests.

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Abdou, D.M., Aziz, E.E., Kazem, Y.S. et al. The diagnostic value of circulating microRNA-499 versus high-sensitivity cardiac troponin T in early diagnosis of ST segment elevation myocardial infarction. Comp Clin Pathol (2021). https://doi.org/10.1007/s00580-021-03204-5

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Keywords

  • MicroRNA-499
  • Troponin T
  • Myocardial infarction