Abstract
This study investigated the effect of African peach (AP) and Uvaria afzelii (UA) extracts on arginase, nitric oxide (NO), angiotensin-1 converting enzyme (ACE), and cholinergic and purinergic enzymes in platelets of complete Freund adjuvant (CFA)-induced arthritic rat model to explain a possible anti-arthritic mechanism of extracts. Sixty adult Wistar female rats (140–280 g) were divided into twelve groups (n = 5). Group I received oral administration of 0.9% NaCl; group II received 10 mg/kg of indomethacin; group III and IV rats received oral administration of 150 and 300 mg/kg of AP extract, respectively; and group V and VI rats received oral administration of 150 and 300 mg/kg of UA extract, respectively. While group VII rats were injected with 0.2 ml CFA (arthritic control rat group), in group VIII, IX, and X, arthritic rats received 10 mg/kg indomethacin, 150 and 300 mg/kg of AP extract orally, respectively, while group XI and XII arthritic rats received 150 and 300 mg/kg of UA extract, respectively. The animals were treated for 21 days. The results revealed decreased NO level, activities of arginase and ACE, and adenosine triphosphate and adenosine monophosphate hydrolyses were significantly decreased while adenosine deaminase activity was significantly decreased in arthritic rat groups administered with different doses of AP and UA extracts. Therefore, AP and UA could be a natural complement and alternative promising anti-arthritic agent.
Similar content being viewed by others
Data availability
The raw/processing data required to reproduce these findings are available but cannot be shared at this time.
References
Adams MR, Moss MO (1999) Food microbiology. The Royal Society of Chemistry, Cambridge, pp 471–476
Adefegha SA, Okeke BM, Oboh G, Ijomone OM, Oyeleye SI (2018) Modulatory effect of eugenol on arginase, nucleotidase, and adenosine deaminase activities of platelets in a carrageenan-induced arthritis rat model: a possible anti-arthritic mechanism of eugenol. Biomedicine & Pharmacotherapy 106:1616–1623
Ayeleso AO, Oguntibeju OO, and Brooks NL (2014) In vitro study on the antioxidant potentials of the leaves and fruits of Nauclea latifolia. Sci World J. Volume 2014, Article ID 437081, 8 pages.
Bennell K, Hinman R (2005) Exercise as a treatment for osteoarthritis. Curr Opin Rheumatol 17:634–640
Bennett JA, Ture SK, Schmidt RA, Mastrangelo MA, Cameron SJ, Terry LE, Yule DI, Morrell CN, Lowenstein CJ (2019) Acetylcholine inhibits platelet activation. Journal of Pharmacology and Experimental Therapeutics 374(1):1–25
Boilard E, Blanco P, Nigrovic PA (2012) Platelets: active players in the pathogenesis of arthritis and SLE. Nat Rev Rheumatol. 8(9):534–542
Boucherle B, Haudecoeur R, Queiroz EF, De Waard M, Wolfender J-L, Robinse RJ, Boumendjel A (2016) Nauclea latifolia: biological activity and alkaloid phytochemistry of a West African tree. Nat. Prod. Rep. 33(9):1034–1043
Bradford M (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein – dye binding. Anal. Biochem. 72:248–254
Braunwald E, Fauci AS, Kasper DL, Hauser SL, Longo DL, James JL (2002) Harrison’s manual of medicine. McGraw-Hill, New York, p 748
Burnstock G (2017) Purinergic signaling in the cardiovascular system. Circ. Res. 120 (1):207– 228. https://doi.org/10.1161/CIRCRESAHA.116.309726
Burnstock GI, Knight GE (2004) Cellular distribution and functions of P2 receptor subtypes in different systems. Int. Rev. Cytol. 240:31–304
Chan KM, Delfert D, Junger KD (1986) A direct colorimetric assay for Ca2+- stimulated ATPase activity. Anal. Biochem. 157:375–380
Cushman DW, Cheung HS (1971) Spectrophotometric assay band properties of the angiotensin-I-converting enzyme of rabbit lung. Biochemical Pharmacology. 20(7):1637–1684
Dawson J, Sedgwick AD, Edwards JC, Lees P (1991) A comparative study of the cellular, exudative and histological responses to carrageenan, dextran and zymosan in the mouse. International Journal of Tissue Reactions. 13(4):171–185
Etukudo I (2003) Ethnobotany, conventional and traditional uses of plants. The Verdict Press, Uyo, p 191
Ezeji-Chigbu, Ngn, Ene Ac, A. A. Emejulu (2018). Sub-acute toxicity evaluation of aqueous stem bark extract of Sarcocephalus Latifolius. @in proceedings{Ngn2018SubAcuteTE} Chemistry.
Guisti G, Galanti B (1984) Colorimetric method. In: Bergemeyer HU (ed) Methods of enzymatic analysis. Weinheim, Verlag Chemie, pp 315–323
Hansra P, Moran EL, Fornasier VL, Bogoch ER (2000) Carrageenan-induced arthritis in the rat. J Inflamm 24:141–155
Harifi G, Sibilia J (2016) Pathogenic role of platelets in rheumatoid arthritis and systemic autoimmune diseases perspectives and therapeutic aspects. Saudi Med J. 37(4):354–360
Italiano JE Jr, Lecine P, Shivdasani RA, Hartwig JH (1999) Blood platelets are assembled principally at the ends of proplatelet processes produced by differentiated megakaryocytes. J Cell Biol. 147:1299–1312
Jin YM, Godfrey DA, Sun Y (2005) Effects of cochlear ablation on choline acetyltransferase activity in the rat cochlear nucleus and superior olive. J Neurosci Res 81:91–101
Kaysen GA, Strecker HJ (1973) Purification and properties of arginase of rat kidney. Biochem. J. 133(4):779–788
Komai N, Morita Y, Sakuta T, Kuwabara A, Kashihara N (2007) Anti-tumor necrosis factor therapy increases serum adiponectin levels with the improvement of endothelial dysfunction in patients with rheumatoid arthritis. Mod Rheumatol 17:385–390
Kumar A, Singh A, Ekavali E (2015) A review on Alzheimer’s disease pathophysiology and its management: an update. Pharmacol. Rep. 67:195–203
Kumar R, Singh S, Saksena AK, Pal R, Jaiswal R, Rahul Kumar R (2019) Effect of Boswellia serrata extract on acute inflammatory parameters and tumor necrosis factor-α in complete Freund’s adjuvant-induced animal model of rheumatoid arthritis. Int J Appl Basic Med Res. 9(2):100–106
Leal DBR, Streher CA, Neu TN, Bittencourt FP, Leal CAM, Silva JEP, Morsch VM, Schetinger MRC (2005) Characterization of NTPDase (NTPDase 1; ectoapyrase; ecto- diphosphohydrolase; CD39; EC 3.6.1.5) activity in human lymphocytes, Biochim. Biophys. Acta 1721:9–15
Leff L (2006) Emerging new therapies in rheumatoid arthritis: what’s next for the patient? J. Infusion Nursing 29:326–337
Lu T-C, Liao J-C, Huang T-H, Lin Y-C, Liu C-Y, Chiu Y-J, Peng W-H. (2009). Analgesic and anti-inflammatory activities of the methanol extract from Pogostemon cablin. eCAM Page 1 of 9.
Lunkes GI, Lunkes DS, Morsch VM, Mazzanti CM, Morsch AL, Miron VR, Schetinger MRC (2004) NTPDase and 5-nucleotidase in rats alloxan–induced diabetes. Diab. Res. Clin. Pract. 65:1–6
Magili ST, Maina HM, Barminas JT, Toma I (2014) Toxicity study of aqueous leaf extracts of Sarcocephalus latifolius (Rubiaceae) in rats. Merit Res J Environ Sci Toxicol. 2(6):120–128
Marcocci L, Marguire JJ, Droy-Lefaiz MT, Parker L (1994) The nitric oxide-scavenging properties of Gingko biloba extract EGB 761. Biochem Biophys Res Commun. 210:748–755
Marcus AJ, Broekman MJ, Drosopoulos JHF, Islam N (1997) The endothelial cell ecto-ADPase responsible for inhibition of platelet function is CD39. Journal of Clinical Investigation 99(6):1351–1360
Marcus AJ, Safier LB, Broekman MJ, Islam N, Fliessbach JH, Hajjar KA, Kaminski WE, Jendraschak E, Silverstein RL, von Schacky C (1995) Thrombosis and inflammation as multicellular processes: significance of cell-cell interactions. Thromb Haemost 74:213–217
Marcus AJ, Safier LB, Hajjar KA, Ullman HL, Islam N, Broekman MJ, Eiroa AM (1991) Inhibition of platelet function by an aspirin-insensitive endothelial cell ADPase: thromboregulation by endothelial cells. J Clin Invest. 88:1690–1696
Mbiantcha M, Almas J, Shabana SU, Nida D, Aisha F (2017) Anti-arthritic property of crude extracts of Piptadeniastrum africanum (Mimosaceae) in complete Freund’s adjuvant-induced arthritis in rats. BMC Complementary and Alternative Medicine 17:111
Nagy Z, Smolenski A (2018) Cyclic nucleotide-dependent inhibitory signaling interweaves with activating pathways to determine platelet responses. Res Pract Thromb Haemost. 2(3):558–571
Ofeimun JO, Eze GI, Okirika OM, Uanseoj SO (2013) Evaluation of the hepatoprotective effect of the methanol extract of the root of Uvaria afzelii (Annonaceae). Journal of Applied Pharmaceutical Science 3(10):125–129
Ohkawa H, Ohishi N, Yagi K (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Ann. J. Biochem 95:351–358
Okeke BM, Adefegha SA, Oyeleye SI, Oboh G (2018) Effects of combined crude alkaloid-rich extracts from alligator pepper (Aframomum melegueta) and bastered melegueta (Aframomum danielli) on the enzymes crucial to erectile dysfunction—in vitro. Journal of Food Biochemistry 42(4):e12550
Orwa C, Mutua A, Kindt R, Jamnadass R. (2009) Agroforestree database: a tree reference and selection guide version 4.0. available at Url: http://www. Worldagroforesty.org/af/treedb/ (accessed on 15 february, 2011).
Perry DG, Hodges EVE, Egan SK (2001) Determinants of chronic victimization by peers: a review and new model of family influence. In: Juvonen J, Graham S (eds) Peer harassment in school: the plight of the vulnerable and victimized. Guilford Press, New York, pp 73–104
Rubin BR (2005) Management of osteoarthritic knee pain. J Am Osteopath Assoc. 105:s23–s28
Sakuta T, Morita Y, Satoh M, Fox DA, Kashihara N (2010) Involvement of the renin– angiotensin system in the development of vascular damage in a rat model of arthritis. Arthritis & Rheumatism. 62(5):1319–1132
Selmi C, De Santisn M, Gershwin ME (2011) Liver involvement in subjects with rheumatic disease. Arthritis Res Ther. 13(3):226
Totoson P, Maguin-Gaté K, Prati C, Wendling D, Demougeot C (2014a) Mechanisms of endothelial dysfunction in rheumatoid arthritis: lessons from animal studies. Arthritis Research & Therapy 16:R22
Totoson P, Maguin-Gaté K, Prati C, Wendling D, Demougeot C (2014b) Mechanisms of endothelial dysfunction in rheumatoid arthritis: lessons from animal studies. Arthritis Res Ther. 16:202
Verhoeven F, Totoson P, Maguin-Gate K, Prigent-Tessier A, Marie C, Wendling D, Moretto J, Prati C, Demougeot C (2016) Glucocorticoids improve endothelial function in rheumatoid arthritis: a study in rats with adjuvant-induced arthritis. Clinical and Experimental Immunology 188:208–218
Zar JH (1984) Biostatistical analysis. Prentice-Hall, Germany, Upper Saddle River, New Jersey
Acknowledgements
The authors appreciate the effort of laboratory assistants and the laboratory in which the work was done.
Funding
This research did not receive any specific grant from funding agencies.
Author information
Authors and Affiliations
Contributions
The conception and design of the study were done by MB Okeke, SA Adefegha, G Oboh, and AO Lawal, while acquisition of data were done by S.A Adefegha and MB Okeke. Data preparation and intrepretation was carried out by SA Adefegha and MB Okeke as well as drafting of the manuscript. All the authors participated in the revising the manuscript and approved the final draft.
Corresponding author
Ethics declarations
Ethics approval
Ethical approval has been reported under animals care in methodology section.
Consent for publication
Not applicable
Competing interests
The authors declare no competing interests.
Code availability
Not applicable
Additional information
Publisher’s note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Okeke, B.M., Adefegha, S.A., Lawal, A.O. et al. Effects of Nauclea latifolia and Uvaria afzelii stem bark extracts on nitric oxide and biomolecules in platelets of complete Freund adjuvant-induced arthritic female rats. Comp Clin Pathol 30, 255–266 (2021). https://doi.org/10.1007/s00580-021-03197-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00580-021-03197-1