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Ameliorative effect of Gongronema latifolium leaf extract on alloxan-induced diabetic cardiomyopathy in Wistar rats model

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Abstract

Diabetic cardiomyopathy (DCM) pathogenesis is complex, and the management options for DCM are limited. This study was designed to investigate the effect of Gongronema latifolium leaf extract on DCM in alloxan-induced diabetic rats. Alloxan monohydrate was used to induce diabetes by an intraperitoneal injection of (150 mg/kg). Three diabetic groups were administered aqueous leaf extract of Gongronema latifolium at 6.36, 12.72, and 25.44 mg/kg bodyweight (BW) respectively; a group was administered with metformin (5 mg/kg BW), while the other two served as positive and negative control. Thereafter, fasting blood glucose, antioxidant enzymes, malondialdehyde level, serum lactate dehydrogenase, creatine kinase-MB, cardiac Troponin I, C-reactive protein, and TNF-α were determined by standard methods. Aqueous extract of Gongronema latifolium leaf significantly (p < 0.05) reduced the alloxan-induced increases in blood glucose, malondialdehyde, serum lactate dehydrogenase, creatine kinase-MB, cardiac Troponin I, C-reactive protein, and tumor necrosis factor-α and increased the alloxan-induced decreases in superoxide dismutase, catalase, glutathione peroxidase, reduced glutathione, and glutathione transferase activities. All these changes were compared with those of metformin-treated diabetic rats. The data from this study suggest that aqueous extract of Gongronema latifolium ameliorated DCM by inhibiting redox imbalance and inflammation in diabetic rats.

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Data availability

The data used to support the findings of this study are available from the corresponding author upon request.

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Acknowledgments

This study was supported by the South African Medical Research Council via funding collected from the South African National Treasury. Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the South African Medical Research Council.

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Authors and Affiliations

  1. Department of Biochemistry, Covenant University, Ota, Ogun State, Nigeria

    Mary Abiola Okesola

  2. Phytomedicine, Natural Products, Drug and Biochemical Toxicology Group, Department of Biochemistry, Landmark University, Omu-Aran, Kwara State, Nigeria

    Oluwafemi Adeleke Ojo

  3. Phytomedicine and Nutraceutical Research Laboratory, Department of Biochemistry, College of Sciences, Afe Babalola University, Ado-Ekiti, Ekiti State, Nigeria

    Oluwafemi Adeleke Ojo, Sunday Amos Onikanni, Basiru Olaitan Ajiboye, Babatunji Emmanuel Oyinloye & Precious Eseose Agboinghale

  4. Biotechnology and Structural Biology (BSB) Group, Department of Biochemistry and Microbiology, University of Zululand, Richards Bay, KwaDlangezwa, 3886, South Africa

    Babatunji Emmanuel Oyinloye

  5. Molecular Biophysics and Structural Biology Group, Department of Biochemistry, Faculty of Science, University of Johannesburg, Johannesburg, South Africa

    Abidemi Paul Kappo

Authors
  1. Mary Abiola Okesola
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  2. Oluwafemi Adeleke Ojo
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Correspondence to Oluwafemi Adeleke Ojo.

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Research involving animals complied with all relevant national regulations and institutional policies of Afe Babalola University for the care and use of animals. This study was approved by the ABUAD Animal Ethical Committee (approval number 19/ABUADSCI/016).

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Okesola, M.A., Ojo, O.A., Onikanni, S.A. et al. Ameliorative effect of Gongronema latifolium leaf extract on alloxan-induced diabetic cardiomyopathy in Wistar rats model. Comp Clin Pathol 29, 865–872 (2020). https://doi.org/10.1007/s00580-020-03134-8

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