Abstract
A cross-sectional study was conducted to determine sero-prevalence of bluetongue virus (BTV) among of different ecological zones of Nepal. A total of 342 goat serum samples were tested by using c-ELISA for the presence of antibodies against BTV. Out of the total 342 serum samples tested, 130 were positive giving an overall sero-prevalence of 38.01%. Significantly higher sero-prevalence was found (p < 0.01) in Rupandehi District (65.11%) compared with Mustang District (25.61%) and Tanahu District (16.30%). Higher sero-prevalence was observed in females (38.43%) compared with their male counterparts (35.41%) but was statistically non-significant. Thus, the goat population in Rupandehi District are at high risk of acquiring bluetongue virus infection. Following an appropriate preventive measures and trans-boundary animal movement regulation can prevent the potential outbreak of disease.
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References
Barduddhe SB, Sundaram RNS, Chakurkar EB, Sahare AM, Swain BK (2005) Ind J Comp Microbiol Immunol Infect Dis 26(1):42–43
Bitew M, Nandi S, Ravishanker C, Somvanshi R (2013) Serological and molecular evidence of bluetongue in sheep and goats in Uttar Pradesh, India. 12(19):2699–2705
Daniel WW (1999) Biostatistics: A Foundation for Analysis in the Health Sciences. 7th edition. New York: John Wiley & Sons
Elbers AR, Popma J, Oosterwolde S, van Rijn PA, Vellema P, van Rooij EM (2008) A cross-sectional study to determine the seroprevalence of bluetongue virus serotype 8 in sheep and goats in 2006 and 2007 in the Netherlands. BMC Res Notes 4(1):33
Gaire T, Karki S, Dhakal IP, Khanal DR, Joshi N, Sharma B, Bowen R (2014) Cross-sectional serosurvey and associated factors of bluetongue virus antibodies presence in small ruminants of Nepal. BMC Res Notes 7:691
Jha VC, Tamang KK (2009) Study of bluetongue disease in sheep in Nepal. Nepalese Vet J 29:95–98
Jha VC, Bista KS, Tamang KK (2008) Bluetongue in sheep in Nepal. Vet Rec 162:288
Joardar SN, Barkataki B, Halder A, Lodh C, Sarma D (2013) Seroprevalence of bluetongue in northeastern Indian State-Assam. Vet World 6(4):196–199
Khalid HS, Laith MS, Abdul-RasoulMazin MN, Mahir HH (2012) A serological surveillance of bluetongue disease in sheep and goats in Iraq by using competitive ELISA technique, Proceeding of eleventh veterinary scientific conference. pp 89–94
Khanal DR, Prajapati M, Shrestha P (2016) Detection of antibodies against bluetongue virus among domestic ruminants in the highlands of Nepal. Vet Ital 52(3–4):313–317
Ma J-G, Zhang X-X, Zheng W-B, Xu Y-T, Zhu X-Q, Hu G-X, Zhou D-H (2017) Seroprevalence and risk factors of bluetongue virus infection in Tibetan sheep and yaks in Tibetan Plateau, China. BioMed Res Int 2017:1–5. https://doi.org/10.1155/2017/5139703
MoLD (2017) Livestock statistics of Nepal. Planning, Monitoring, and Evaluation Division, Singhadurbar, Kathmandu, Nepal
Mozaffari AA, Khalili M (2012) The first survey for antibody against bluetongue virus in sheep flock in southeast of Iran. Asian Pac J Trop Biomed 2:S1808–S1810
Mozaffari AA, Khalili M, Sabahi S, (2014) High seroprevalence of bluetongue virus antibodies in goats in southeast Iran. Asian Pac J Trop Biomed 4, pp S275–S278
Panda MK, Mondal A, Joardar SN (2011) Seroprevalence of Bluetongue Virus in Sheep, Goat and Cattle in West Bengal, India. Animal Sci Rep 5(3):105–110
Pany S, Chand K, Biswas SK, Mondal B, Panda HK (2018) Prevalence of bluetongue virus antibodies in small ruminants of coastal Odisha. Ind J Small Rum 24:171. https://doi.org/10.5958/0973-9718.2018.00030.2
Prasad G, Jain NC, Gupta Y (1992) Bluetongue virus infection in India: a review. Rev Sci Tech 11(3):699–711
Puri B (2014) Seroprevalence of bluetongue disease among ruminant livestock in Darchula district of Far-western Nepal. Thesis report of M.V.Sc Tribhuvan University, IAAS, Rampur, Chitwan, Nepal
Purse BV, Mellor PS, Rogers DJ, Sauel AR, Mertens PPC, Baylis M (2005) Climatic change and the recent emergency of bluetongue in Europe. Nat Rev Microbiol 3:171–181
Ravishankar C, Nair GK, Mini M, Jayaprakasan V (2005) Seroprevalence of bluetongue virus antibodies in sheep and goats in Kerala State, India. Rev Sci Tech 24:953–958
Sabaghan M, Borujeni MP, Seifi Abad Shapouri MR, Rasooli A, Norouzi M, Samimi S, Mansouri S (2014) Seroprevalence of bluetongue in sheep in Kohgiluyeh and Boyer-Ahmad province. Iran Vet Research For 5(4):325–328
Sharma R, Beckford S, Tiwari K, Vinet E, Thomas D, De Allie C, Chikweto A (2016) Sero-prevalence of bluetongue virus antibody in ruminants from Grenada. Open J Vet Med 06:99–103. https://doi.org/10.4236/ojvm.2016.66013
Shlash KH, Abdual-Rasoul L MS, Naji MM, Hussain MH (2012) A serological surveillance of bluetongue disease in sheep and goats in Iraq by competitive ELISA technique. In: Proceedings of Eleventh Veterinary Scientific Conference. pp 889–941
Sreenivasulu D, Subba Rao MV, Reddy YN, Gard GP (2004) Overview of bluetongue disease, viruses, vectors, surveillance and unique features: the Indian subcontinent and adjacent regions. Vet Ital 40:73–77
Sun C, Huang EP, Qingyuan L, Xu W, Haixiu XM, Lu X, Li PJ, Liu W, Bu WG, Wu ZL (2016) Emergence of a novel bluetongue virus serotype, China 2014. J Transbound Emerg Dis 63:585–589. https://doi.org/10.1111/tbed.12560
World Organization for Animal Health Organization (2008) Manual of standards for diagnostic tests and vaccines
Yadhav AK (2012) Seroprevalence of bluetongue in some of the sheep flocks in Lalitpur, Bhaktapur, Bhojpur, Dhanusa and Kaski districts. Mini-thesis report for B. V. Sc and A. H, Purbanchal University, Biratnagar
Yeh JY, Kim JG, Choi J, Kim JK, Kim KW (2018) Bluetongue virus antibodies in domestic goats: a countrywide and retrospective study in the Republic of Korea. Vector-Borne Zoon Dis 18(6):323–330
Zhang N, Li Z, Zhang F, Zhu J (2004) Studies of bluetongue disease in the People’s Republic of China. Vet Ital 40(3):51–56
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This study was funded by the Institute of Agriculture and Animal Science (IAAS), Tribhuvan University, under the Internship Program Management Fund.
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Parajuli, S., Acharya, K.P. Detection of antibodies against bluetongue virus among goats of different eco-climatic zones of Nepal. Comp Clin Pathol 28, 1177–1180 (2019). https://doi.org/10.1007/s00580-019-02992-1
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DOI: https://doi.org/10.1007/s00580-019-02992-1