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Assessment of antioxidant and cutaneous wound healing effects of Falcaria vulgaris aqueous extract in Wistar male rats

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Abstract

Treatment of wounds by ethnomedicinal plants which have fewer side effects than chemical drugs has been on the rise. In this experiment, we evaluated cutaneous wound healing potential of aqueous extract of Falcaria vulgaris in Wistar male rats. DPPH free radical scavenging test was used to examine the antioxidant effect of F. vulgaris aqueous extract, which indicated high antioxidant activity compared to butylated hydroxy toluene (BHT) as the positive control. In our study, after creating the cutaneous wound on the back of the rats, the animals were randomly divided into four groups; untreated control, treatment with Eucerin ointment, treatment with 3% tetracycline ointment, treatment with 3% F. vulgaris aqueous extract ointment. The groups were treated for 30 days. For histopathological and biochemical analysis of the cutaneous wound healing trend, a 3 × 3 cm section was prepared from all dermal thicknesses at days 10, 20, and 30. The use of F. vulgaris aqueous extract ointment in the treatment groups led to significant decrease (p < 0.05) in the levels of wound area, total cells, lymphocyte, neutrophil, macrophage, and significant enhance (p < 0.05) in the levels of wound contracture, hydroxyproline, hexosamine, fibrocyte, fibroblast, and the rate of fibrocyte to fibroblast as compared to the control and basal ointment groups. According to the results, F. vulgaris aqueous extract ointment can treat the cutaneous wound.

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Authors and Affiliations

  1. Department of Toxicology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

    Samaneh Goorani & Mohammad Kazem Koohi

  2. Department of Clinical Science, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran

    Mohammad Mahdi Zangeneh, Niloofar Seydi, Akram Zangeneh, Narges Souri & Marziye-Sadat Hosseini

  3. Biotechnology and Medicinal Plants Research Center, Ilam University of Medical Sciences, Ilam, Iran

    Mohammad Mahdi Zangeneh & Akram Zangeneh

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  1. Samaneh Goorani
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  2. Mohammad Mahdi Zangeneh
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Correspondence to Mohammad Mahdi Zangeneh.

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Goorani, S., Zangeneh, M.M., Koohi, M.K. et al. Assessment of antioxidant and cutaneous wound healing effects of Falcaria vulgaris aqueous extract in Wistar male rats. Comp Clin Pathol 28, 435–445 (2019). https://doi.org/10.1007/s00580-018-2866-3

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  • DOI: https://doi.org/10.1007/s00580-018-2866-3

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