Abstract
The phytochemical constituents, antioxidant, and cytotoxicity as well as the inhibitory potentials of Clerodendrum glaburum on key carbohydrate metabolizing enzymes were investigated. The plant sample was extracted separately with hexane, EtOAc, MeOH, and water. The phytochemical analysis and antioxidant assays of the extracts were achieved using standard procedures; the antidiabetic capability of the extracts against the actions of α-amylase and α-glucosidase was examined while their cytotoxicity was tested against Vero cells. Highest quantity of phenol (65.97 mg gallic acid g−1), flavonoid (47.02 mg quercetin g−1), and flavanol (173.74 mg catechin g−1) were observed in MeOH extract. Also, MeOH extract had the most potent ability (p < 0.05) to scavenge ABTS (0.05 mg/mL), DPPH (0.17 mg/mL), and superoxide anion (0.36 mg/mL) than other extracts and standards. Stronger inhibition (p < 0.05) against metal chelation (26.41 mg/mL) and FRAP (5.92 mg/mL) were observed in water extract compared to other extracts; it also competes favorably with the standard. EtOAc extract displayed best scavenging potentials (p < 0.05) against hydroxyl radical than other extracts. Methanol (0.71 mg/mL) and aqueous (0.19 mg/mL) extracts displayed more potent inhibition against the actions of α-amylase and α-glucosidase respectively compared to acarbose and other extracts. Hexane extract displayed better antidiabetic activities as revealed by its moderate α-amylase (2.54 mg/mL) and potent α-glucosidase (0.53 mg/mL) inhibitions compared to acarbose. The hexane, methanol, and aqueous extracts were non-toxic against Vero cells, with LC50 of 0.11, 0.23, and 0.52 mg/mL respectively. C. glaburum leaves contain active phytochemicals that can be beneficial in managing diabetes and other oxidative stress-induced disorders.
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References
Adisakwattana S, Charoenlertkul P, Yibchok-Anun S (2009) Alpha-glucosidase inhibitory activity of cyanidin-3-galactoside and synergistic effect with acarbose. J Enzyme Inhib Med Chem 24:65–69
American Diabetes Association (2011) Diagnosis and classification of diabetes mellitus. Diabetes Care 34:62–69
Apostolidis E, Kwon YI, Shetty K (2007) Inhibitory potential of herb, fruit, and fungal-enriched cheese against key enzymes linked to type 2 diabetes and hypertension. Innovative Food Sci Emerg Technol 8:46–54
Aslan M, Orhan N, Orhan DD, Ergun F (2010) Hypoglycemic activity and antioxidant potential of some medicinal plants traditionally used in Turkey for diabetes. J Ethnopharmacol 128:384–389
Babior BM (2000) Phagocytes and oxidative stress. Am J Med 109:33–44
Bagri P, Ali M, Aeri V, Bhowmik M, Sultana S (2009) Antidiabetic effect of Punica granatum flowers: effect on hyperlipidemia, pancreatic cells lipid peroxidation and antioxidant enzymes in experimental diabetes. Food Chem Toxicol 47:50–54
Benzie IFF, Strain JJ (2014) The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Anal Biochem 239:70–76
Braca A, Tommasi ND, Bari LD, Pizza C, Politi M, Morelli I (2001) Antioxidant principles from Bauhinia terapotensis. J Nat Prod 64:892–895
Chika A, Bello SO (2010) Antihyperglycaemic activity of aqueous leaf extract of Combretum micranthum (Combretaceae) in normal and alloxan-induced diabetic rats. J Ethnopharmacol 129:34–37
Copeland RA (2000) Enzymes: a practical introduction to structure, mechanism and data analysis. Wiley-VCH, New York
Dinis TCP, Madeira VMC, Almeida IM (1994) Action of phenolic derivatives (acetaminophen, salycilate and 5-aminosalycilate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Arch Biochem Biophys 315:161–169
Dnyaneshwar MN, Archana RJ (2013) In vitro inhibitory effects of Pithecellobium dulce (Roxb.) Benth. Seeds on intestinal α-glucosidase and pancreatic α-amylase. J Biochem Technol 4:616–621
Edeoga HO, Okwu DE, Mbaebie BO (2005) Phytochemical constituents of some Nigerian medicinal plants. Afr J Biotechnol 47:685–688
Elisha IL, Dzoyem JP, McGaw LJ, Botha FS, Eloff JN (2016) The anti-arthritic, anti-inflammatory, antioxidant activity and relationships with total phenolics and total flavonoids of nine South African plants used traditionally to treat arthritis. BMC Complement Altern Med 16:307
Elsnoussi AHM, Mohammad JAS, Lee FA, Sadikun A, Chan SH, Tan S (2012) Potent α-glucosidase and α-amylase inhibitory activities of standardized 50% ethanolic extracts and sinen set in from Orthosiphon stamineus Benth as antidiabetic mechanism. BMC Complement Altern Med 12:176
Fong DS, Aiello LP, Ferris FL 3rd, Klein R (2004) Diabetic retinopathy. Diabetes Care 27:2540–2253
Fouad MA, Wanas AS, Khalil HE (2013) Phytochemical and biological studies of Clerodendrum Glabraum leaves. Int J Pharmacogn Phytochem 28(2):1164–11688
Fowler MJ (2008) Microvascular and macrovascular complications of diabetes. Clin Diabetes 26(2):77–82
Gil MI, Tomas-Barberan FA, Hess-Pierce B, Holcroft DM, Kader AA (2000) Antioxidant activity of pomegranate juice and its relationship with phenolic composition and processing. J Agric Food Chem 48:4581–4589
Gülçin I, Elmastaş M, Aboul-Enein HY (2007) Determination of antioxidant and radical scavenging activity of basil (Ocimum basilicum L. family Lamiaceae) assayed by different methodologies. Phytother Res 21(4):354–361
Hye YK, Byung HM, Hak JL, Don HC (2004) Flavonol glycosides from the leaves of Eucommia ulmoides O with glycation inhibitory activity. J Ethnopharmacol 93(2–3):227–230
Kajaria D, Tripathi JS, Tiwari SK (2013) Phytochemical composition and in vitro antimicrobial and antioxidant activities of anti-asthmatic polyherbal compounds. Elixir Hum Physiol 6:16666–16671
Kazeem MI, Abimbola SG, Ashafa AOT (2013) Inhibitory potential of Gossypium arboreum leaf extracts on diabetes key enzymes, α-amylase and α-glucosidase. Bangladesh J Pharmacol 8:149–155
Krentz AJ, Baile CJ (2005) Oral antidiabetic agents: current role in type 2 diabetes mellitus. Drugs 65:385–411
Kumaran A, Karunakaran RJ (2007) In vitro antioxidant activities of methanol extracts of Phyllantus species from India. Lebensm Wiss Technol 40:344–352
Kwon YI, Apostolidis E, Shetty K (2008) In vitro studies of eggplant (Solanum melongena) phenolics as inhibitors of key enzymes relevant for type 2 diabetes and hypertension. Bioresour Technol 99:2981–2988
Liang N, Kitts DD (2014) Antioxidant property of coffee components: assessment of methods that define mechanisms of action. Molecules 19:19180–19208
Liu X, Kim JK, Li Y, Li J, Liu F, Chen X (2005) Tannic acid stimulates glucose transport and inhibits adipocyte differentiation in 3T3-li cells. J Nutr 135(2):165–171
Liu F, Ooi VEC, Chang ST (1997) Free radical scavenging activity of mushroom polysaccharide extracts. Life Sci 60:763–771
Mahomoodally F (2013) Traditional medicines in Africa: an appraisal of ten potent African medicinal plants. Evid Based Complement Alternat Med 2013:1–14
Masevhea NA, Awouafack MD, Ahmed AS, McGaw LJ, Eloff JN (2013) Clerodendrumic acid, a new triterpenoid from Clerodendrum glabrum(Verbenaceae), and antimicrobial activities of fractions and constituents. Helv Chim Acta 96:1693–1703
Mccue PP, Shetty K (2004) Inhibitory effects of rosmarinic acid extracts on porcine pancreatic α-amylase in vitro. Asia Pac J Clin Nutr 13:101–106
McGaw LJ, Steenkamp V, Eloff JN (2007) Evaluation of Athrixia bush tea for cytotoxicity, antioxidant activity, caffeine content and presence of pyrrolizidine alkaloids. J Ethnopharmacol 110:16–22
Miliauskas G, Yenkutonis PR, Vanbeek TA (2004) Screening of radical scavenging activity of some medicinal and aromatic plants extracts. Food Chem 85:231–237
Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity. J Immunol Methods 65:55–63
Müller L, Fröhlich K, Böhm V (2011) Comparative antioxidant activities of carotenoids measured by ferric reducing antioxidant power (FRAP), ABTS bleaching assay (αTEAC), DPPH assay and peroxyl radical scavenging assay. Food Chem 129(1):139–148
Oboh G, Puntel RL, Rocha JBT (2007) Hot pepper (Capsicum annuum, Tepin and Capsicum chinese, Habanero) prevents Fe2+ induced lipid peroxidation in brain: in vitro. Food Chem 102:178–185
Ogundajo AL, Kazeem MI, Owoyele OA, Ogunmoye AO, Ogunwande IA (2016) Inhibition of α-amylase and α-glucosidase by Acanthus montanus leaf extracts. Br J Pharm Res 9:1–8
Raymond MH, Sandy A, Andrey LB et al (2004) “Labiatae” the families and genera of vascular plants, volume VII. Springer-Verlag, Germany
Re R, Pellegrini N, Proteggente A (1994) Antioxidant activity applying an improved ABTS radical cation decolourisation assay. Free Radic Biol Med 26:1231–1237
Samatha T, Shyamsundarachary R, Srinivas RS (2012) Quantification of total phenolic and total flavonoid contents in extracts of Oroxylum indicum L. Kurz Asian J Pharm Clin Res 5:177–179
Schmidt E, Lotter M, McCleland W (2002) Trees and shrubs of Mpumalanga and Kruger National Park. Jacana Media
Sindhu M, Emilia Abraham T (2005) In vitro antioxidant activity and scavenging effects of Cinnamonium verum leaf extract assayed by different methodologies. Food Chem Toxicol 44:198–206
Sofowora EA (1982) Medicinal plants and traditional medicine in Africa. John Wiley and Sons, New York
Steven JW, Laura H, Russ S, Patrick AR, Richard GO (1998) Phylogeny in Labiataes. L., inferred from cpDNA sequences. Plant Syst Evol 209:265–274
Suba V, Murugesan T, Rao RB, Ghosh L, Pal M, Mandal SC, Saha BP (2004) Antidiabetic potential of Barleria lupulina extract in rats. Fitoterapia 75:1–4
Tadera K, Minami Y, Takamatsu K, Matsuoka T (2006) Inhibition of alpha-glucosidase and alpha-amylase by flavonoids. J Nutr Sci Vitaminol 52:149–153
Thomas V, Grant R, Van Gogh J (2004) Sappi tree spotting Highveld and Drakensberg. Jacana Media, South Africa
Trease GE, Evans WC (1978) Pharmacognosy. Bailliere Tindall Limited, United Kingdom
Tundis R, Loizzo MR, Menichini F (2010) Natural products as α-amylase and α-glucosidase inhibitors and their hypoglycaemic potential in the treatment of diabetes. Mini-Rev Med Chem 10:315–331
Tung YCL, Rimmington D, O’Rahilly S, Coll AP (2007) Pro-opiomelanocortin (POMC) modulates the thermogenic and physical activity responses to high fat feeding and markedly influences dietary fat preference. J Endocrinol 148:5331–5338
Van Wyk BE, Van Oudtshoorn B, Gericke N (2009) Turning folklore into an ethnomedicinal catalogue Medicinal Plants of South Africa. Briza, South Africa
VanWyk B, VanWyk P (1997) Field guide to trees of Southern Africa. Struik, South Africa
Wolfe K, Wu X, Liu RH (2003) Antioxidant activity of apple peels. J Agric Food Chem 51:609–614
Yang CY, Wang J, Zhao Y, Shen L, Jiang X, Xie ZG, Liang N, Zhang L, Chen ZH (2010) Anti-diabetic effects of Panax notoginseng saponins and its major anti-hyperglycemic components. J Ethnopharmacol 130:231–236
Zheng T, Shu G, Yang Z, Mo S, Zhao Y, Mei Z (2012) Antidiabetic effect of total saponins from Entada phaseoloides (L.) Merr. In type 2 diabetic rats. J Ethnopharmacol 139:814–821
Zheng W, Wang SY (2001) Antioxidant activity and phenolic compounds in selected herbs. J Agric Food Chem 49:5165–5170
Zirihi GN, Mambu L, Guede-Guina F, Bodo B, Grellier P (2005) In vitro antiplasmodial activity and cytotoxicity of 33 West African plants used for treatment of malaria. J Ethnopharmacol 98:281–285
Acknowledgments
We acknowledge the technical assistance of Dr. Lateef Ariyo Adeniran.
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This study was funded by Directorate Research Development, University of Free state, South Africa (Entity No. 2114 B5004).
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Akintayo Lanre Ogundajo was awarded Postdoctoral fellowship from Directorate Research Development, University of Free state, South Africa for (Entity No. 2114 B5004) tenable at the Phytomedicine and Phytopharmacology Research Unit of the Department of Plant Sciences, University of the Free State (UFS), Qwaqwa Campus, Phuthaditjhaba, South Africa.
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Ogundajo, A.L., Ashafa, A.O.T. Medicinal properties of Clerodendrum glaburum E may leaf extracts: phytochemical constituents, antioxidant, cytotoxicity, and carbohydrate-metabolizing enzyme inhibitory potentials. Comp Clin Pathol 28, 927–936 (2019). https://doi.org/10.1007/s00580-018-2825-z
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DOI: https://doi.org/10.1007/s00580-018-2825-z