Comparative Clinical Pathology

, Volume 27, Issue 5, pp 1161–1165 | Cite as

Phylogenetic analysis of Anaplasma marginale and Anaplasma ovis isolated from small ruminant based on MSP4 gene in western regions of Iran

  • Ali Yousefi
Original Article


Anaplasma is a genus of rickettsiales bacteria which is considered as a member of the tick-borne diseases. Different species of this genus are transmitted by blood-sucking insects and arthropods and blood-contaminated instruments. The present study was carried out to determine the prevalence of Anaplasma ovis and Anaplasma marginale and to perform phylogenetic analysis in goat and sheep in East of Kermanshah provinces, western region of Iran. From April 2015 to May 2016, a total of 360 blood samples of small ruminants were collected. In the first step, DNA extraction and in the following nested PCR and the amplification of MSP4 gene were done and a total of 32.78% (118/360) of small ruminants were positive for Anaplasma species. The obtained nucleotide sequences were analyzed and phylogenetic tree was constructed. The similarity among the nucleotide sequences of A. ovis in this study was 100%, and for A. marginale, it showed 99% identity with the others. Statistical analysis by chi-square test did not show any significant relationship between the presence of Anaplasma species and statistical variables like age, tick infestation, sex, and location (p > 0.05). Therefore, further studies are required in different areas of Iran to ascertain our knowledge and to verify Anaplasma species isolates.


Anaplasma marginale Anaplasma ovis Tick-borne Sheep Goat Kermanshah Iran 



The authors would like to thank Mrs. Fatemeh Khademi, Mis. Fariba nuori, Mr. Abbas Yousefi, and Mr. Masoud Moradi for their help in the collection of samples.


This study was funded by the Science and Research Branch of Islamic Azad University, Tehran.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.


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Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Young Researchers and Elites Club, Science and Research BranchIslamic Azad UniversityTehranIran

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