Comparative Clinical Pathology

, Volume 27, Issue 5, pp 1155–1160 | Cite as

Seroprevalence of brucellosis in different kinds of feline population in north-east of Iran

  • M. T. GaroussiEmail author
  • J. Mehrzad
  • A. Baniassadi
  • J. Khoshnegah
Original Article


The objective of this survey was to assess the prevalence of brucellosis with focus on Brucella abortus and Brucella melletensis in different kinds of cats in north-east of Iran. Different kinds of cats included stray, pet, and cats which were located in dairy cattle herds tested. Blood sera of 48 (34.28%) stray, 42 (30%) pet, and 50 (35.71%) cats collected from 15 industrial dairy cattle herds. They were used for rapid and tube agglutination tests such as Rose Bengal, Wright, and 2-mercaptoethanol (2-ME). The comparison among age, sex, weight, and lifestyle of cats with serological tests of brucellosis were examined statistically. Totally, eight (5.71%) cats were mild positive with low titer. Only two (1.42%) cats reacted in 2-ME. There was no statistically significant relationship between sex of cat (male vs female) and serological results (P = 0.07). However, comparison among different age groups revealed significant differences (p = 0.009). There were no significant differences between the infected cats collected from dairy cattle herds and stray cats, but the stray cats and pet cats showed the significant differences (p = 0.028). This survey showed that Brucella infection (1) mainly occurred in male and stray cats aged 1–2 years and (2) is transiently mild, and (3) negative brucellosis in pet cats represents optimal hygiene and health monitoring of these cats by the owners. So, stray cats can be a risk factor for brucellosis in human and animal populations in north-east of Iran; thus, strong precautions over stray cats are necessary.


Feline Brucellosis Brucella abortus Brucella melitensis Prevalence Seroepidemiology 



We appreciate Eng. Delarm Talebkhan Garoussi for drawing the cat trap.

Funding information

This research was financially supported by the vice chancellor of research, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad-Iran. The grant number was 347.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of cats were strictly followed.


  1. Alton GG, Jones RD, Verger JM (1988) Techniques for the brucellosis laboratory. Institut National de la Recherche Agronomique (INRA) 147:13–61Google Scholar
  2. Bagheri Nejad, R, Talebkhan Garoussi. M, Dabiri, H, Niasari, A and Jalali, H. (2016). Isolation of Brucella abortus biovar 5 from camel fresh milk in Iran. The 1st international & 4th national congress of Entric pathogens. Karadj-Iran. Feb. 15-17. Page: 19Google Scholar
  3. Brown J, Blue JL, Wooley RE, Dreesen DW, Carmichael LE (1976) A serologic survey of a population of Georgia dogs for Brucella canis and an evaluation of the slide agglutination test. J Am Vet Med Assoc 169:1214–1216PubMedGoogle Scholar
  4. Carmichael LE. (1979). Brucellosis (Brucella canis). In: Steele JH, editor. Handbook series in zoonoses, Sect. A, v. l. Boca Raton, FL: CRC Press Inc. P. 185–94Google Scholar
  5. Carmichael LE, Shin SJ (1996) Canine brucellosis: a diagnostician’s dilemma. Semin Vet Med Surg (Small Animal) J 11:161–165CrossRefGoogle Scholar
  6. Constable PD, Hinchcliff KW and Done SH, Grunberg W. (2017). Veterinary Medicine. Elsevier publication 11th edition. p. 1761Google Scholar
  7. Ferreira AC, Cardoso R, Travassos-Dias I, Mariano I, Belo A, Rolao-Preto I, Manelga A, Pina-Fonseca A, Correa DE, Sa MI (2003) Evaluation of a modified Rose Bengal test and an indirect enzyme-linked immunosorbent assay for the diagnosis of Brucella melitensis infection in sheep. Vet Res 34:297–305CrossRefPubMedGoogle Scholar
  8. Flores-Castro R and Carmichael LE. (1977). Canine brucellosis: current status of methods for diagnosis and treatment. In: 27th Gaines veterinary symposium. p. 17–24Google Scholar
  9. Ghorbani A, Rabbani Khorasgani M, Hamid Zarkesh-Esfahani Z, Hassan Sharifiyazdi H, Dehghan Kashani A, Emami H (2013) Comparison of serology, culture, and PCR for detection of brucellosis in slaughtered camels in Iran. Comp Clin Pathol 22(5):913–917CrossRefGoogle Scholar
  10. Greene CE, and Carmichael LE. (2006). Canine brucellosis. In: Greene CE, editor. Infectious diseases of the dog and cat. WB Saunders Co. P. 369–81Google Scholar
  11. Hollett RB (2006) Canine brucellosis: outbreaks and compliance. Theriogenology 66:575–587CrossRefPubMedGoogle Scholar
  12. Morgan WTB, McDiarmid A (1960) The excretion of Brucella abortus in the milk of experimentally infected cattle. Res Vet Sci 1:53–56Google Scholar
  13. Palmer MV, Cheville NF (1997) Effects of oral or intravenous inoculation with Brucella abortus strain RB51 vaccine in beagles. Am J Vet Res 58(8):851–856PubMedGoogle Scholar
  14. Radostitis MO, Gay CC, Hinchcliff KW and Constable PC. (2007). Veterinary medicine. Elseevier publication. 10th edition. p. 699Google Scholar
  15. Randhawa AS, Dieterich WH, Hunter CC, Kelly VP, Johnson TC, Svoboda B (1977) Prevalence of seropositive reactions to Brucella canis in a limited survey of domestic cats. J Am Vet Med Assoc 171:267–268PubMedGoogle Scholar
  16. Repina LP, Nikulina AI, Kosilov IA (1993) A case of human infection with brucellosis from a cat. Zh Mikrobiol Epidemiol Immunobiol 4:66–68Google Scholar
  17. Talebkhan Garoussi M, Bagherinejad R, Dabiri M and Niasarinaslji A. The sero-epidemiology survey of Brucellosis in milking camel. (2015). The 2nd international & 6th national Iranian congress of Brucellosis. Tehran- Iran. 11-13. Nov. 38Google Scholar
  18. Talebkhan Garroussi M, Firoozi S, Nowrouzian (1997) The serological survey of Brucella abortus and Melitensis in shepherd dogs around Mashhad farms. J Fac Vet Med Univ Tehran 51(3&4):55–62Google Scholar
  19. Talebkhan Garoussi M, Haghparast AR, Hajenejad MR (2009) Seroprevalence and epidemiological aspects of bovine viral diarrhoea virus infection in dairy cattle herds in suburb of Mashhad-Iran. Trop Anim Health Prod 41:663–667CrossRefPubMedGoogle Scholar
  20. Talebkhan Garoussi M, Haghparast AR, Rafati MS (2011) Prevalence of bovine viral diarrhoea virus in persistently infected cows in industrial dairy herds of Mashhad suburb-Iran. Int J Vet Res 5(4):198–203Google Scholar
  21. Thrusfield, M. (2005). Veterinary epidemiology. Third ed. Blackwell Science Publication. p. 233Google Scholar
  22. Tobias G, Tobias T, Abood S, Hamor R, Ballam J (1998) Determination of age in dogs and cats by use of changes in lens reflections and transparency. Am J Vet Res 59:945–950PubMedGoogle Scholar
  23. Wanke MM (2004) Canine brucellosis. Anim Reprod Sci 82–3:195–207CrossRefGoogle Scholar

Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of TheriogenologyFaculty of Veterinary Medicine University of TehranTehranIran
  2. 2.Section of Immunology, Department of Microbiology & Immunology, Faculty of Veterinary MedicineUniversity of TehranTehranIran
  3. 3.Institute of BiotechnologyFerdowsi University of MashhadMashhadIran
  4. 4.Department of Clinical Sciences, Faculty of Veterinary MedicineFerdowsi University of MashhadMashhadIran

Personalised recommendations