Comparative Clinical Pathology

, Volume 27, Issue 4, pp 847–853 | Cite as

Haemocytomorphometric variation in different ages of Japanese quail, Coturnix coturnix japonica (Temminck and Schlegel, 1849)

  • Sujata Puspamitra
  • Prafulla K. Mohanty
  • Bandi K. Mallik
Original Article


The investigation focuses to assess the haematological and cytomorphometric variation in Japanese quail (Coturnix coturnix japonica) at different age groups with sexual dimorphism. Two millilitres of blood from 60 individual birds, comprising 10 males and 10 females from three age groups, was collected from ulnar vein of Japanese quail. Haematological and morphometric values of the blood samples were determined by using standard protocols. For haematology and morphometry, three age groups were considered, i.e., chick (0–3 weeks), grower (4–5 weeks), and adult (above 5 weeks), respectively. Data were analysed by following standard statistical protocol both for ANOVA and test of significance. The data were presented as mean ± standard error. Significant difference both in age and sex has been recorded at p < 0.01. The results of blood cells reflect a difference at p < 0.01 and cell morphometry in experimental birds were similar to that of chickens and other birds.


Haematology Cytomorphometry Japanese quail Coturnix coturnix japonica 



The investigators owe their gratitude to the Head, PG Department of Zoology, Utkal University, Vani Vihar, Bhubaneswar-751 004, Odisha, for facilitating the laboratory studies. Authors are also grateful to the Director of the Central Poultry Development Organisation (CPDO), Government of India poultry farm of Eastern Region, Bhubaneswar, Odisha, for extending necessary help in collection of samples from the farm. The first author acknowledges University Grants Commission for granting Rajiv Gandhi National Fellowship as a support to curry out the research.

Authors’ contribution

Sujata Puspamitra, significance: sample collection, paper writing, and data interpretation

Prof. Prafulla K. Mohanty, significance: paper correction

Bandi K. Mallik, significance: rechecking of statistical data and interpretation


The programme was funded by the University Grants Commission-New Delhi under RGNF bearing file number F1-17.1/2013-14/RGNF-2013-14-SC-ORI-51259/(SA-III/Website).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

The guidelines applicable for the investigation have been appropriately followed international, national, and/or institutional guidelines for the care and use of animals were followed. As per the discussion with the members of the Animal Ethical Committee of the University, the permission of the ethical committee is not necessary since the animals are neither sacrificed nor killed nor anesthetised nor narcotized nor etherised nor harmed during the investigation. Further, for this particular investigation, only 2 ml of blood per species of different age groups and different sexes is required which is collected during the routine test of the birds of Central Poultry Development Organization, Govt. of India, Odisha. This neither causes any injury nor pain to the birds as blood is collected by professional veterinary doctors of the farm.


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Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Cytogenetics Laboratory, Post Graduate Department of ZoologyUtkal UniversityBhubaneswarIndia
  2. 2.Central Poultry Development Organisation (CPDO)Eastern Region, Govt. of IndiaBhubaneswarIndia

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