Haemocytomorphometric variation in different ages of Japanese quail, Coturnix coturnix japonica (Temminck and Schlegel, 1849)

  • Sujata Puspamitra
  • Prafulla K. Mohanty
  • Bandi K. Mallik
Original Article
  • 15 Downloads

Abstract

The investigation focuses to assess the haematological and cytomorphometric variation in Japanese quail (Coturnix coturnix japonica) at different age groups with sexual dimorphism. Two millilitres of blood from 60 individual birds, comprising 10 males and 10 females from three age groups, was collected from ulnar vein of Japanese quail. Haematological and morphometric values of the blood samples were determined by using standard protocols. For haematology and morphometry, three age groups were considered, i.e., chick (0–3 weeks), grower (4–5 weeks), and adult (above 5 weeks), respectively. Data were analysed by following standard statistical protocol both for ANOVA and test of significance. The data were presented as mean ± standard error. Significant difference both in age and sex has been recorded at p < 0.01. The results of blood cells reflect a difference at p < 0.01 and cell morphometry in experimental birds were similar to that of chickens and other birds.

Keywords

Haematology Cytomorphometry Japanese quail Coturnix coturnix japonica 

Notes

Acknowledgements

The investigators owe their gratitude to the Head, PG Department of Zoology, Utkal University, Vani Vihar, Bhubaneswar-751 004, Odisha, for facilitating the laboratory studies. Authors are also grateful to the Director of the Central Poultry Development Organisation (CPDO), Government of India poultry farm of Eastern Region, Bhubaneswar, Odisha, for extending necessary help in collection of samples from the farm. The first author acknowledges University Grants Commission for granting Rajiv Gandhi National Fellowship as a support to curry out the research.

Authors’ contribution

Sujata Puspamitra, significance: sample collection, paper writing, and data interpretation

Prof. Prafulla K. Mohanty, significance: paper correction

Bandi K. Mallik, significance: rechecking of statistical data and interpretation

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

The guidelines applicable for the investigation have been appropriately followed international, national, and/or institutional guidelines for the care and use of animals were followed. As per the discussion with the members of the Animal Ethical Committee of the University, the permission of the ethical committee is not necessary since the animals are neither sacrificed nor killed nor anesthetised nor narcotized nor etherised nor harmed during the investigation. Further, for this particular investigation, only 2 ml of blood per species of different age groups and different sexes is required which is collected during the routine test of the birds of Central Poultry Development Organization, Govt. of India, Odisha. This neither causes any injury nor pain to the birds as blood is collected by professional veterinary doctors of the farm.

References

  1. Ali MA, Hmar L, Inaotombi LD, Prava M, Lallianchhunga MC, Tolenkhomba TC (2012) Effect of age on the haematological and bio-chemical profile of Japanese quails (Coturnix coturnix japonica). Int Multidiscip Res J 2(8):32–35Google Scholar
  2. Baumgartner J (1993) Japanese quail production, breeding and genetics. Proceedings of the 10th International Symposium on current problems of avian genetics. Nitra, Solvakia, pp101–103Google Scholar
  3. Blue-Mclendon A, Green RA (2010) Hematology of ratites. In: Weiss DJ, Wardrop KJ (eds) Schalm’s veterinary hematology, 6th edn. Lippincott Williams and Wilkins, Ames, pp 987–993Google Scholar
  4. Bounous DI, Stedman NL (2000) Normal avian haematology: chicken and turkey. In: Feldman BV, Zinkl JG, Jain NC (eds) Schalm’s veterinary hematology. Williams and Wilkins, Philadelphia, pp 1145–1154Google Scholar
  5. Campbell TW (1988) Avian hematology and cytology. Iowa State University Press, Ames, pp 3–27Google Scholar
  6. Campbell T (1995) Avian haematology and cytology, 2nd edn. Iowa State University Press, Ames, pp 3–19Google Scholar
  7. Cardoso ALSP, Tessari ENC (2003) Estudo dos parâmetros hematológicos em frangos de corte. Arq Inst Biol 70(4):419–424Google Scholar
  8. Dacie JV, Lewis SM, Luzzatto L (1995) Laboratoratory methods used in the investigation of paroxysmal nocturnal haemoglobinuria (PHN). In: Dacie JV, Lewis SM (eds) Practical haematology, 8th edn. Churchill Livingstone, London, pp 287–296Google Scholar
  9. Dalai M, Puspamitra S, Bhattacherjee A, Acharya D, Acharya G, Mohanty PK (2015) Comparative haematology of Anas platyrhynchos (Anseriformes) and Coturnix coturnix japonica (Galliformes). J Entomol Zool Stud 3(5):50–53Google Scholar
  10. Fortes EAM, Sousa AF, Almeida ECS, Conde JAM, Moura WL (2009) Morfologia das células do sangue periférico em emas (Rhea americana). Braz J Vet Res Anim Sci 46(3):215–221CrossRefGoogle Scholar
  11. Galloa SSM, Ederlib NB, Boa-Mortea MO, Oliveiraa FCR (2015) Hematological, morphological and morphometric characteristics of blood cells from rhea, Rhea Americana (Struthioniformes: Rheidae): a standard for Brazilian birds. Braz J Biol 75(4):953–962CrossRefGoogle Scholar
  12. Hodges RD (1974) The histology of the fowl. Academic Press, London, pp 150–171Google Scholar
  13. Hodges RD (1977) Comparative clinical haematology. Blackwell Scientific Publications, Oxford, pp 483–517Google Scholar
  14. Islam MS, Lucky NS, Islam MR, Ahad A, Das BR, Rahman MM, Siddiui MSI (2004) Haematological parameters of Fayoumi, Assil and local chickens reared in Sylhet region in Bangladesh. Int J Poult Sci 3(2):144–147CrossRefGoogle Scholar
  15. Latimer SL,Dorothee B (2010) Schalm’s veterinary hematology. Chaper III. In: Weiss DJ, Wardrop KJ (eds) 6th ed, pp 345Google Scholar
  16. Lucas AM, Jamroz C (1961) Atlas of Avian Haematology, United States Department of Agriculture, vol 25. Agricultural Monograph, Washington, pp 211–215Google Scholar
  17. Mcinroy RA (1953) A micro-haematocrit for determining the packed cell volume and haemoglobin concentration on capillary blood. J Clin Pathol 7:32–36CrossRefGoogle Scholar
  18. Mihailov R, Lasheva V, Lashev L (1999) Some hematological values in Japanese quails. Bulg J Vet Med 2:137–139Google Scholar
  19. Minvielle F (2004) The future of Japanese quail for research and production. World Poult Sci J 60:500–507CrossRefGoogle Scholar
  20. Mukherjee DP, Ayyagari VB, Panda B (1971) Sex difference in the morphology of erythrocyte nucleus and melanising activity of blood of one-day-old chicks. Br J Poultry Sci 12:459–462CrossRefGoogle Scholar
  21. Olorode BR, Longe OG (2000) Effect of replacing palm kernel cake with shear butter cake on quality characteristics, haematology and serum chemistry of laying hens. Niger J Anim Prod 27:19–23Google Scholar
  22. Ritchie BW, Harrison GJ, Harrison LR (1994) Avian medicine: principles and application. Wingers Publishing, Inc., Florida, pp 874–888Google Scholar
  23. Sahli H, Lehrbuchd K (1909) Untersuchungen Methode, 5th edn. Leipsic, p 84Google Scholar
  24. Shave HJ, Howard V (1976) A hematologic survey of captive waterfowl. J Wildl Dis 12(2):195–201CrossRefPubMedGoogle Scholar
  25. Sinha RC (1983) Haematological studies on the prewintering and wintering frog, Rana sculenta. Comp Biochem Psysiol 71(2):311–314CrossRefGoogle Scholar
  26. Sonia C, Asha RR, Vairamuthu S (2012) Haematological parameters of pearl guinea fowl influenced by rearing system, age and sex. India J Poult Sci 47(3):395–397Google Scholar
  27. Tadjalli M, Nazifi S, Said M (1997) Morphological studies on the blood of healthy native north Iranian green-head ducks. J Fac Vet Med, Uni Tehran No 1 and 2(51):113–12Google Scholar
  28. Tadjalli M, Nazifi S, Eemanparvar A (2003) Normal cellular morphology of the blood of Japanese quail (Coturnix coturnix japonica). Comp Clin Pathol 12:102–105CrossRefGoogle Scholar

Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Cytogenetics Laboratory, Post Graduate Department of ZoologyUtkal UniversityBhubaneswarIndia
  2. 2.Central Poultry Development Organisation (CPDO)Eastern Region, Govt. of IndiaBhubaneswarIndia

Personalised recommendations