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A histopathological study of the healing potential of Plantago lanceolata ointment on collagenase-induced tendonitis in rats

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Abstract

The purpose of this study was to evaluate Plantago lanceolata (PL) ointment as a topical treatment of collagenase-induced tendonitis in rat, histopathologically. The study was performed on 24 male Wistar rats kept in the same environmental conditions. The animals were randomly assigned to four groups of six rats, and 2.5 units of bacterial collagenase was injected at mid-mass of right and left Achilles complexes. The right legs were considered as treatment and the left ones as control. On treatment legs, 0.1 g PL ointment and on control legs the same amount of eucerin (the vehicle of the ointment) were applied and massaged for 2 min. Each group of animals was euthanized at the end of the first, second, third, and fourth weeks. The Achilles complex of both legs was harvested, processed for hematoxylin and eosin staining, and studied under light microscopy. During the 4 weeks, in the treatment group, significant proliferation of endotendon, earlier disappearance of adipose, inflammatory cells and tendon lobulation, and faster collagen fiber rearrangement were observed.

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Acknowledgments

The authors would like to thank Mr. S. Hasanzadeh for preparation of the histopathological slides.

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Authors and Affiliations

  1. Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, 7616914111, Iran

    Mohammad Mehdi Oloumi

  2. Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran

    Amin Derakhshanfar

  3. Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran

    Sina Kakooei & Laya Golmoradi

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  1. Sina Kakooei
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  2. Mohammad Mehdi Oloumi
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  3. Amin Derakhshanfar
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  4. Laya Golmoradi
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Correspondence to Mohammad Mehdi Oloumi.

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Kakooei, S., Oloumi, M.M., Derakhshanfar, A. et al. A histopathological study of the healing potential of Plantago lanceolata ointment on collagenase-induced tendonitis in rats. Comp Clin Pathol 22, 977–981 (2013). https://doi.org/10.1007/s00580-012-1512-8

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  • DOI: https://doi.org/10.1007/s00580-012-1512-8

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