, Volume 28, Issue 7, pp 691–701 | Cite as

Mycelium of Terfezia claveryi as inoculum source to produce desert truffle mycorrhizal plants

  • Francisco Arenas
  • Alfonso Navarro-Ródenas
  • Daniel Chávez
  • Almudena Gutiérrez
  • Manuela Pérez-Gilabert
  • Asunción MorteEmail author
Original Article


Terfezia claveryi Chatin was the first desert truffle species to be cultivated, the mycorrhizal plants being successfully produced by using both desert truffle spores and mycelia. However, it is more advisable to use mycelium than spores whenever possible and profitable. Given the low yields of mycelia obtained using traditional culture methods of this truffle, the medium composition was modified in an attempt to determine its nutritional requirements. For this, an assay involving response surface methodology was performed using Box-Behnken design to find the optimal parameters for the high production of mycelial biomass. The best results were obtained with glucose as carbon source, buffering the pH at 5 during culture, adding a pool of vitamins, and adjusting the optimal concentrations of carbon and nitrogen sources of the MMN medium. Biomass production increased from 0.3 to 3 g L−1 dry weight and productivity increased from 10.7 to 95.8 mg L−1 day−1 dry weight. The produced mycelium was able to colonize Helianthemum roots efficiently, providing more than 50% ectomycorrhizal colonization.


Mycelial biomass Box-Behnken design Response surface methodology Desert truffle Terfezia claveryi 



This work was supported by projects 19484/PI/14 (Fundación Séneca of Region of Murcia, FEDER, Spain) and CGL2016-78946-R (AEI-FEDER, UE). F. Arenas thanks the Ministerio de Economía y Competitividad (MINECO) for financial resources from the Youth Employment Initiative (IEJ) and the European Social Fund (FSE) (PEJ-2014-A-83659). D. Chávez would like to thank the Chilean National Council for Science and Technology CONICYT 21110038 for the assigned postgraduate grant. A. Navarro-Ródenas is grateful to the University of Murcia for a postdoctoral contract.

Supplementary material

572_2018_867_MOESM1_ESM.jpg (61 kb)
Fig. S1 Summary of the different experiments performed. (JPG 61 kb)
572_2018_867_MOESM2_ESM.docx (91 kb)
Fig. S2 Parameter profile during mycelial growth of Terfezia claveryi in bioreactor. Symbols for the parameters used: pH (short dash), mL of NaOH added (dotted) and dissolved oxygen (%DO) (solid). (DOCX 90 kb)
572_2018_867_MOESM3_ESM.docx (14 kb)
Table S1 (DOCX 14 kb)
572_2018_867_MOESM4_ESM.docx (13 kb)
Table S2 (DOCX 13 kb)
572_2018_867_MOESM5_ESM.docx (13 kb)
Table S3 (DOCX 13 kb)
572_2018_867_MOESM6_ESM.docx (13 kb)
Table S4 (DOCX 13 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Departamento de Biología Vegetal, Facultad de BiologíaUniversidad de MurciaMurciaSpain
  2. 2.Departamento de Ciencias y Tecnología Vegetal, Campus Los ÁngelesUniversidad de Concepción, Escuela de Ciencias y TecnologíasLos AngelesChile
  3. 3.Departamento de Bioquímica y Biología Molecular A, Facultad de VeterinariaUniversidad de MurciaMurciaSpain

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