, Volume 28, Issue 7, pp 679–690 | Cite as

Ectomycorrhizae formed by three Japanese truffle species (Tuber japonicum, T. longispinosum, and T. himalayense) on indigenous oak and pine species

  • Akihiko KinoshitaEmail author
  • Keisuke Obase
  • Takashi Yamanaka
Original Article


Modern truffle cultivation systems started in Europe in the early 1970s and are now successfully used for several European truffles throughout the world. However, systems for indigenous novel truffle species need to be developed in several regions, especially where truffle cultivation has not been attempted so far, such as in Japan. Recently, two new and one known truffle species that are expected to be edible were reported from Japan: Tuber japonicum, T. longispinosum, and T. himalayense. Here, we conducted mycorrhization trials between these three truffle species and four native tree species in Japan (Quercus acutissima, Q. phillyraeoides, Q. serrata, and Pinus densiflora) using spore suspension and trap-plant seedling techniques under axenic pot culture conditions to understand their potential host ranges and mycorrhizal morphologies and to determine whether these inoculation methods are applicable for mycorrhization of Japanese truffles with native host plants. Of the 12 combinations, nine were successful for mycorrhization, including both oak and pine trees. The T. japonicum mycorrhiza was characterized by short, needle-shaped cystidia without septa, whereas the two black truffles, T. longispinosum and T. himalayense, were indistinguishable from each other because they shared the same morphological and anatomical characters such as brownish, long cystidia with right angle ramification. These features were similar to related black truffle species. The results of the present study indicate that the inoculation method used for European truffles can also be applied for mycorrhization between Japanese truffle species and compatible native pine and/or oak hosts in Japan.


Mycorrhization Pinus Quercus Truffle cultivation Tuberaceae 


Funding information

This work was supported by a grant from the Ministry of Agriculture, Forestry and Fisheries of Japan, entitled “Technology development for the optimal use of forest resources.” We thank Junichi Abe Peter, Hideo Hara, and Minoru Nakajima for collecting samples. We thank Robbie Lewis, MSc, from Edanz Group (, for editing a draft of this manuscript.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Kyushu Research CenterForestry and Forest Products Research InstituteKumamotoJapan
  2. 2.Department of Mushroom Science and Forest MicrobiologyForestry and Forest Products Research InstituteIbarakiJapan

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