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SNP discovery in radiata pine using a de novo transcriptome assembly

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A set of single-nucleotide polymorphisms (SNPs) was discovered using a de novo transcriptome for radiata pine. The reference transcriptome and SNPs were annotated according to the functional information available in biological databases.

A set of SNPs was discovered using a de novo transcriptome assembly for radiata pine in Chile. De novo transcriptome was generated by Illumina sequences and assembled by Trinity. A set of unique genes and transcription factors were identified. All dataset sequences were mapped against the de novo reference transcriptome and SNPs were called. A total of 300,761 SNPs were identified between samples, where 38,730 SNPs had a functional annotation. Since there is little data available of radiata pine-omics, the aim of this work is to provide to the scientific community a de novo transcriptome assembly and a set of SNPs.

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Acknowledgements

This project was financed by Genómica Forestal SA.

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Correspondence to Sofía Valenzuela.

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The authors declare that they have no conflicts of interest.

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The data have been submitted to the NCBI. The bioproject ID related to this paper is PRJNA495832.

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Communicated by Daniel G. Peterson.

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Durán, R., Rodriguez, V., Carrasco, A. et al. SNP discovery in radiata pine using a de novo transcriptome assembly. Trees 33, 1505–1511 (2019). https://doi.org/10.1007/s00468-019-01875-w

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  • DOI: https://doi.org/10.1007/s00468-019-01875-w

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