Whole-genome re-sequencing reveals molecular mechanisms of biomass changes in 11-year-old Bt transgenic poplar
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Genome-wide insertions/deletions were identified in Bt transgenic poplar through whole-genome re-sequencing, revealing the molecular mechanisms underlying changes in the growth and wood characteristics of Bt transgenic poplar.
Poplar is a fast-growing tree that relieves the wood demand in China. Previously, we found growth and wood characteristics differed in some lines of insect-resistant Bt transgenic poplar. Here, we selected five Bt transgenic lines (#12, #153, #172, #208 and #209), one untransformed control (CK2), one comparable control (CK3), and one native cultivar (JY, Populus × euramericana ‘Robusta’) in Xinjiang, China, for further study. Based on breast diameter, tree height, chemical content, and fibre morphology analyses, these transgenic lines showed fast growth and high-lignin and low-cellulose contents in the cell wall. In addition, genome-wide insertions/deletions (InDels) were compared between two transgenic lines (#12 and #153) and the untransformed control via whole-genome re-sequencing. GO enrichment and protein–protein interaction networks indicated the genes affected by differential InDels were related to programmed cell death and defence responses. This study provides new insight into the molecular mechanisms underlying changes in biomass characteristics in Bt transgenic poplar.
KeywordsTransgenic poplar Bt Wood characteristic Whole-genome re-sequencing InDels Protein–protein interaction network
Cinnamyl alcohol dehydrogenase
Mitogen-activated protein kinase
Programmed cell death
We thank Dr. Rongfeng Huang for the wood characteristics and chemical content measurement. The work was supported by the National Key Research and Development Program of China (2017YFD0600201) and the National Key Program on Transgenic Research (2018ZX08021001).
JH, JZ and ML conceived and designed the experiments. JZ, TZ, HJ and LW participated in the experiments. JZ and TZ performed data analysis and drafted the manuscript. JH provides reagents and analysis tools. All authors read and approved the final version of the manuscript.
Compliance with ethical standards
Conflict of interesr
The authors declare no competing financial interest.
- Chen Y, Han Y, Li L, Tian Y, Nie S (1994) Study on the plant regeneration from Populus deltoides explant transformed with Bt. toxin gene. Scientia Silvae Sinicae 31:97–103Google Scholar
- Escudero V, Jordá L, Sopeña-Torres S, Mélida H, Miedes E, Muñoz-Barrios A, Swami S, Alexander D, McKee LS, Sánchez-Vallet A (2017) Alteration of cell wall xylan acetylation trigger defense responses that counterbalance the immune deficiencies of plants impaired in the β subunit of the heterotrimeric G protein. Plant J 92(3):386–399CrossRefGoogle Scholar
- Heinze B (1997) A PCR marker for a Populus deltoides allele and its use in studying introgression with native European Populus nigra. Belg J Bot 129(2):123–130Google Scholar
- Hu J, Wang K, Han Y, Liu Q, Zhang B, Tian Y (1999) Field test on insect-resistance of transgenic plants (Populus nigra) transformed with Bt toxin gene. For Res 12:202–205Google Scholar
- Hu J, Wang L, Yan D, Lu M (2014) Research and application of transgenic poplar in China. Challenges and Opportunities for the World’s Forests in the 21st Century. Springer, Dordrecht, pp. 567–584Google Scholar
- Schouten HJ, vande Geest H, Papadimitriou S, Bemer M, Schaart JG, Smulders MJ, Perez GS, Schijlen E (2017) Re-sequencing transgenic plants revealed rearrangements at T-DNA inserts, and integration of a short T-DNA fragment, but no increase of small mutations elsewhere. Plant Cell Rep 36:493–504CrossRefGoogle Scholar
- Schweingruber FH (2007) Wood structure and environment. Springer, Berlin Heidelberg, New YorkGoogle Scholar
- Tian Y, Li T, Mang K, Han Y, Li L, Wang X, Lu M, Dai L, Yan J (1992) Insect tolerance of transgenic Populus nigra plants transformed with Bacillus thuringiensis toxin gene. Chin J Biotechnol 9:219–227Google Scholar
- Van der Does D, Boutrot F, Engelsdorf T, Rhodes J, McKenna JF, Vernhettes S, Koevoets I, Tintor N, Veerabagu M, Miedes E (2017) The Arabidopsis leucine-rich repeat receptor kinase MIK2/LRR-KISS connects cell wall integrity sensing, root growth and response to abiotic and biotic stresses. PLoS Genet 13:e1006832CrossRefGoogle Scholar
- Wang X, Han Y, Dai L, Li L, Tian Y (1997) Studies on insect-resistant transgenic (P. x Euramericana) plants. Scientia Silvae Sinicae 33:74–81Google Scholar
- Wheeler EA, Baas P, Gasson PE (1989) IAWA list of microscopic features for hardwood identificationGoogle Scholar
- Wu N, Fan Y (1991) Establishment of engineered poplars containing Bacillus thuringiensis δ-endotoxin gene. Chin Sci Bull 9:705–708Google Scholar