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Expression profiling of HbWRKY1, an ethephon-induced WRKY gene in latex from Hevea brasiliensis in responding to wounding and drought

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Abstract

A full-length WRKY cDNA, designated as HbWRKY1, was isolated from the latex in Hevea brasiliensis. The deduced HbWRKY1 contained 303 amino acid residues with one WRKY domain and one zinc-finger motif, and shared high identity to WRKYs from other plant species. The phylogenetic tree showed that HbWRKY1 was classified into group-II WRKYs. Many cis-acting elements related to hormone and stress responses were identified in the 1,377 bp-length 5′ regulatory sequence of HbWRKY1, including three W-boxes, three ABREs, two MYBCOREs, one PRE, two OSE2ROOTNODULEs, one SEBFCONSSTPR10A and two HSEs. Semi-quantitative RT-PCR analysis revealed more significant accumulation of HbWRKY1 transcripts in latex and flowers than in bark, leaves and roots. The transcription of HbWRKY1 in latex was strongly induced by tapping and some wounding-signal molecules such as ethylene and jasmonic acid, and Oidium heveae attack, PEG and abscisic acid also strongly induced its expression in the leaves of H. brasiliensis. All these results suggest that HbWRKY1 mainly functions in responding to mechanical wounding, some wounding-related hormones, drought and O. heveae attack as an ethephon-induced WRKY transcriptional factor in H. brasiliensis.

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Abbreviations

ABA:

Abscisic acid

ET:

Ethylene

ETH:

Ethephon

JA:

Jasmonic acid

RACE:

Rapid amplification of cDNA ends

RT-PCR:

Reverse transcription-polymerase chain reaction

SSH:

Suppression subtractive hybridization

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No.30760197 and No.31000313) and National Nonprofit Institute Research Grant of CATAS-ITBB (ITBB110201) in partial coverage of the costs.

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Authors and Affiliations

  1. Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, China

    Quanqi Zhang, Jiahong Zhu, Yanmei Ni, Yuanbao Cai & Zhili Zhang

  2. Tropical Crop Research Institute, Guang Dong State Farm, Maoming, 525140, China

    Quanqi Zhang & Yanmei Ni

  3. Hainan Academy of Agricultural Sciences, Haikou, 571000, China

    Zhili Zhang

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  1. Quanqi Zhang
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  2. Jiahong Zhu
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  3. Yanmei Ni
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  4. Yuanbao Cai
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  5. Zhili Zhang
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Correspondence to Zhili Zhang.

Additional information

Communicated by F. Canovas.

Q. Zhang and J. Zhu contributed equally to this work.

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Zhang, Q., Zhu, J., Ni, Y. et al. Expression profiling of HbWRKY1, an ethephon-induced WRKY gene in latex from Hevea brasiliensis in responding to wounding and drought. Trees 26, 587–595 (2012). https://doi.org/10.1007/s00468-011-0623-1

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  • DOI: https://doi.org/10.1007/s00468-011-0623-1

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