Advances in urinary proteome analysis and biomarker discovery in pediatric renal disease

Abstract

Recent progress in proteomic analysis and strategies for the identification of clinically useful biomarkers in biofluids has led to the identification of urine as an excellent non-invasive reservoir for biomarkers of disease. Urinary biomarkers have been identified and validated on independent cohorts in different high-incidence adult renal diseases, including diabetic nephropathy, chronic kidney disease and immunoglobulin A-nephropathy, but also in extrarenal disease, such as coronary artery disease. Unfortunately, this type of research is underrepresented in the pediatric population. Here, we present the rare studies in the pediatric population that identified potential clinically useful urinary biomarkers in ureteropelvic junction (UPJ) obstruction and renal Fanconi syndrome. These studies, although limited in number, clearly show the potential of urinary proteomics, especially in the pediatric population. It is anticipated that the advances made in the adult population, the lessons learned on the use of appropriate statistics and the inclusion of independent blinded validation cohorts in these types of studies will rapidly lead to clinical useful urinary biomarkers for other pediatric (renal) disease in a population where non-invasive analysis is particularly appreciated.

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Acknowledgments

CC, CL, SD and JPS acknowledge financial support from the Agence Nationale pour la Recherche (ANR-07-PHYSIO-004-01), the Fondation pour la Recherche Médicale “Grands Equipements pour la Recherche Biomédicale” and the CPER2007–2013 programme. The work of SD was sponsored by the Inserm Interface program. HM was supported in part by EUROTRANS-BIO grant ETB-2006-016 and EU Funding through InGenious HyperCare (LSHM-C7-2006-037093) and PREDICTIONS (1272568). JPS was supported by Inserm, the “Direction Régional Clinique” (CHU de Toulouse, France) under the Interface program and by the Fondation pour la Recherche Médicale.

Conflict of interest statement

Harald Mischak is the co-founder and co-owner of Mosaiques Diagnostics, who developed the CE-MS technology for clinical applications.

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Correspondence to Joost P. Schanstra.

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Caubet, C., Lacroix, C., Decramer, S. et al. Advances in urinary proteome analysis and biomarker discovery in pediatric renal disease. Pediatr Nephrol 25, 27–35 (2010). https://doi.org/10.1007/s00467-009-1251-5

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Keywords

  • Biomarkers
  • Fanconi syndrome
  • Proteomics
  • Statistics
  • Ureteropelvic junction obstruction
  • Urine
  • Validation