We evaluated the protective effects of antioxidant at the onset of fever on renal damage in a rat model of acute pyelonephritis. Twenty rats were allocated to four groups. In groups 1 to 3, the animals were given direct inoculation of Escherichia coli into the right kidney, and group four served as control. All rats in groups 1 to 3 were given once-daily intraperitoneal injections of ceftriaxon for five consecutive days, beginning on the third day after inoculation. The animals’ body temperatures were monitored; as soon as body temperature reaches 38°C, the rats in group 2 were given allopurinol co-treatment, whereas, in group 3, vitamin E co-treatment was started at fever onset. Both kidneys were excised 6 weeks later, for the evaluation of histopathologic changes, apoptotic damage, and concentrations of transforming growth factor-beta (TGF-β). Only minimal changes were found in control samples. Pathologic scores of inflammation and fibrosis in group 1 were higher than in the vitamin E and allopurinol groups (P < 0.05). Apoptosis index was also decreased in groups 2 and 3, compared to group 1 (P < 0.05). There was no significant difference in average TGF-β levels between study groups. These findings suggest that administration of vitamin E or allopurinol following the onset of fever can reduce renal damage in pyelonephritis.
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- E. coli :
urinary tract infection
enzyme immunosorbent assay
transforming growth factor-beta
terminal deoxynucleotidyl transferase deoxyuridine triphosphate (dUTP) nick-end labeling
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Special thanks to Drs. Rostambeigy, Vejdani, Elmi, Rahbar, and Salmasi, and to Miss Aligholy and Mr. Farzaneh, for their kind help.
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Sadeghi, Z., Kajbafzadeh, AM., Tajik, P. et al. Vitamin E administration at the onset of fever prevents renal scarring in acute pyelonephritis. Pediatr Nephrol 23, 1503–1510 (2008). https://doi.org/10.1007/s00467-008-0853-7
- Acute pyelonephritis
- Renal scar
- Vitamin E
- Cell apoptosis