An aerobic denitrifying bacterium, stain LK-618, was isolated from lake sediment surface and the efficacy of inorganic nitrogen removal was tested. Stain LK-618 identified as Pseudomonas sihuiensis by 16S rRNA sequencing analysis. Trisodium citrate was found to be the ideal carbon source for this strain. When an initial nitrogen sources of approximately 50 mg/L nitrate, ammonium, or nitrite was solely selected as the nitrogen source, the nitrogen removal efficiencies were 91.4% (3.86 mg/L/h), 95.07% (2.47 mg/L/h) and 97.7% (2.41 mg/L/h), respectively. Nitrogen balance analysis revealed that 55.12% NO3−-N was removed as N2. Response surface methodology (RSM) analysis demonstrated that the optimal Total Nitrogen (TN) removal ratio for strain LK-618 was under C/N ratio of 12.63, shaking speed of 52.06 rpm, temperature of 28.5 °C and pH of 6.86. In addition, strain LK-618 could tolerate NaCl concentrations up to 20 g/L, and its most efficient denitrification capacity was presented at NaCl concentrations of 0–10 g/L. Therefore, strain LK-618 has potential application on the removal of inorganic nitrogen from saline wastewater under aerobic conditions.
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Yang M, Lu D, Qin B, Liu Q, Zhao Y, Liu H, Ma J (2018) Highly efficient nitrogen removal of a coldness-resistant and low nutrient needed bacterium, Janthinobacterium sp. M-11. Bioresour Technol 256:366–373
Bai H, Liao S, Wang A, Huang J, Shu W, Ye J (2019) High-efficiency inorganic nitrogen removal by newly isolated Pannonibacter phragmitetus B1. Bioresour Technol 271:91–99
Robertson LA, Niel EWJ, Torremans RAM, Kuenen JG (1988) Simultaneous nitrification and denitrification in aerobic chemostat culture of Thiosphaera pantropha. Appl Environ Microbiol 54:2812–2818
Patureau D, Davison J, Bernet N, Moletta R (1994) Denitrification under various aeration conditions in Comamonas sp., strain SGLY2. FEMS Microbiol Ecol 14:71–78
Rout PR, Bhunia P, Dash RR (2017) Simultaneous removal of nitrogen and phosphorous from domestic wastewater using Bacillus cereus GS-5 strain exhibiting heterotrophic nitrification, aerobic denitrification and denitrifying phosphorous removal. Bioresour Technol 244:484–495
Zhang N, Chen H, Lyu YK, Wang Y (2019) Nitrogen removal by a metal-resistant bacterium, Pseudomonas putida ZN1, capable of heterotrophic nitrification-aerobic denitrification. J Chem Technol Biotechnol 94(4):1165–1175
Lei Y, Wang Y, Liu H, Xi C, Song L (2016) A novel heterotrophic nitrifying and aerobic denitrifying bacterium, Zobellella taiwanensis DN-7, can remove high-strength ammonium. Appl Microbiol Biotechnol 100:4219–4229
Sun Y, Feng L, Li A, Zhang X, Yang J, Ma F (2017) Ammonium assimilation: an important accessory during aerobic denitrification of Pseudomonas stutzeri T13. Bioresour Technol 23:4264–4272
Zhao B, He YL, Huang J, Taylor S, Hughes J (2010) Heterotrophic nitrogen removal by Providencia rettgeristrain YL. J Ind Microbiol Biot 37(6):609–616
Nakano M, Inagaki T, Okunishi S, Tanaka R, Maeda H (2010) Effect of salinity on denitrification under limited single carbon source by Marinobacter sp. Isolated from marine sediment. J Basic Microbiol 50:285–289
Li Y, Wang Y, Fu L, Gao Y, Zhao H, Zhou W (2017) Aerobic-heterotrophic nitrogen removal through nitrate reduction and ammonium assimilation by marine bacterium Vibrio sp. Y1–5. Bioresour Technol 230:103–111
Xi D, Li C, Tang R, Lv Z, Ren Y, Wei C, Feng C (2014) Ion-exchange membrane bioelectrochemical reactor for removal of nitrate in the biological effluent from a coking wastewater treatment plant. Electrochem Commun 46:99–102
Yu H, Li J, Dong HY, Qiang ZM (2019) Nitrogen removal performance of marine anammox bacteria treating nitrogen-rich saline wastewater under different inorganic carbon doses: high inorganic carbon tolerance and carbonate crystal formation. Bioresour Technol 288:8
Cheng X, Liang Y, Banuelos G, Yan B, Yu X, Zhu H, Zhou Q (2016) Constructed wetlands for saline wastewater treatment: a review. Ecol Eng 98:275–285
Zhao C, Zhang H, Song C, Zhu JK, Shabala S (2020) Mechanisms of plant responses and adaptation to soil salinity. Innovation. https://doi.org/10.1016/j.xinn.2020.100017
Zhang QL, Liu Y, Ai GM, Miao LL, Zheng HY, Liu ZP (2012) The characteristics of a novel heterotrophic nitrification-aerobic denitrification bacterium. Bacillus methylotrophicus strain L7. Bioresour Technol 108:35–44
Wu ZD, Huang SB, Yang YL, Xu FQ, Zhang YQ, Jiang R (2013) Isolation of an aerobic denitrifying bacterial strain from a biofilter for removal of nitrogen oxide. Aerosol Air Qual Res 13:1126–1132
Qing H, Oscar OD, Tian C, Wang C, Wu X, Feng S, Liu Y, Xiao B (2018) Novel heterotrophic nitrogen removal and assimilation characteristic of the newly isolated bacterium Pseudomonas stutzeri AD-1. J Biosci Bioeng 126:339–345
Hong P, Wu X, Shu Y, Wang C, Tian C, Wu H, Xiao B (2020) Bioaugmentation treatment of nitrogen-rich wastewater with a denitrifier with biofilm-formation and nitrogen-removal capacities in a sequencing batch biofilm reactor. Bioresour Technol 303:122905
Zhao B, Cheng DY, Tan P, An Q, Guo JS (2018) Characterization of an aerobic denitrifier Pseudomonas stutzeri strain XL-2 to achieve efficient nitrate removal. Bioresour Technol 250:564–573
Huang T, Guo L, Zhang H, Su J, Wen G, Zhang K (2015) Nitrogen-removal efficiency of a novel aerobic denitrifying bacterium, Pseudomonas stutzeri strain ZF31, isolated from a drinking-water reservoir. Bioresour Technol 196:209–216
Guo Y, Zhou X, Li Y, Li K, Wang C, Liu J, Yan D, Liu Y, Yang D, Xing J (2013) Heterotrophic nitrification and aerobic denitrification by a novel Halomonas campisalis. Biotechnol Lett 35:2045–2049
Duan J, Fang H, Su B, Chen J, Lin J (2015) Characterization of a halophilic heterotrophic nitrification-aerobic denitrification bacterium and its application on treatment of saline wastewater. Bioresour Technol 179:421–428
Zhao B, Tian M, An Q, Ye J, Guo JS (2017) Characteristics of a heterotrophic nitrogen removal bacterium and its potential application on treatment of ammonium-rich wastewater. Bioresour Technol 226:46–54
Liu Y, Ai GM, Miao LL, Liu ZP (2016) Marinobacter strain NNA5, a newly isolated and highly efficient aerobic denitrifier with zero N2O emission. Bioresour Technol 206:9–15
Joo HS, Hirai M, Shoda M (2005) Characteristics of ammonium removal by heterotrophic nitrification-aerobic denitrification by Alcaligenes faecalis no. 4. J Biosci Bioeng 100:184–191
Takaya N, Catalan-Sakairi MAB, Sakaguchi Y, Kato I, Zhou Z, Shoun H (2003) Aerobic denitrifying bacteria that produce low levels of nitrous oxide. Appl Environ Microbiol 69:3152–3157
Shi Z, Zhang Y, Zhou J, Chen M, Wang X (2013) Biological removal of nitrate and ammonium under aerobic atmosphere by Paracoccus versutus LYM. Bioresour Technol 148:144–148
Rajakumar S, Ayyasamy PM, Shanthi K, Thavamani P, Velmurugan P, SongLakshmanaperumalsamy YCP (2008) Nitrate removal efficiency of bacterial consortium (Pseudomonas sp. KW1 and Bacillus sp. YW4) in synthetic nitrate-rich water. J Hazard Mater 157:553–563
Su JJ, Yeh KS, Tseng PW (2006) A Strain of Pseudomonas sp. isolated from piggery wastewater treatment systems with heterotrophic nitrification capability in Taiwan. Curr Microbiol 53:77–81
Jin R, Liu T, Liu G, Zhou J, Huang J, Wang A (2015) Simultaneous heterotrophic nitrification and aerobic denitrification by the marine origin bacterium Pseudomonas sp. ADN-42. Appl Biochem Biotechnol 175(4):2000–2011
Wang T, Dang Q, Liu C, Yan J, Fan B, Cha D, Yin Y, Zhang Y (2016) Heterotrophic nitrogen removal by a newly-isolated alkalitolerant microorganism, Serratia marcescens W5. Bioresour Technol 211:618–627
Chen J, Xu J, Zhang S, Liu F, Peng J, Peng Y, Wu J (2021) Nitrogen removal characteristics of a novel heterotrophic nitrification and aerobic denitrification bacteria, Alcaligenes faecalis strain WT14. J Environ Manage 282:111961
Zhao B, An Q, He YL, Guo JS (2012) N2O and N2 production during heterotrophic nitrification by Alcaligenes faecalis strain NR. Bioresour Technol 116:379–385
Wan C, Yang X, Lee DJ, Du M, Wan F, Chen C (2011) Aerobic denitrification by novel isolated strain using NO2–N as nitrogen source. Bioresour Technol 102:7244–7248
Sun Z, Lv Y, Liu Y, Ren R (2016) Removal of nitrogen by heterotrophic nitrification-aerobic denitrification of a novel metal resistant bacterium Cupriavidus sp. S1. Bioresour Technol 220:142–150
Ji B, Yang K, Zhu L, Jiang Y, Wang HY, Zhou J, Zhang HN (2015) Aerobic denitrification: a review of important advances of the last 30 years. Biotechnol Bioprocess Eng 20:643–665
Huang HK, Tseng SK (2001) Nitrate reduction by Citrobacter diversus under aerobic environment. Appl Microbiol Biotechnol 55:90–94
Guo LJ, Zhao B, An Q, Tian M (2016) Characteristics of a novel aerobic denitrifying bacterium, Enterobacter cloacae Strain HNR. Appl Biochem Biotechnol 78:947–959
Uygur A, Kargi F (2004) Salt inhibition on biological nutrient removal from saline wastewater in a sequencing batch reactor. Enzyme Microb Technol 34(3–4):313–318
Vyrides I, Stuckey DC (2009) Adaptation of anaerobic biomass to saline conditions: role of compatible solutes and extracellular polysaccharides. Enzyme Microb Technol 44(1):46–51
Ji B, Yang K, Wang H, Zhou J, Zhang H (2015) Aerobic denitrification by Pseudomonas stutzeri C3 incapable of heterotrophic nitrification. Bioprocess Biosyst Eng 38:407–409
This work was supported by the National Natural Science Foundation of China (grant number 31670465, 31800112, 31800390 and 31870450), Advanced Talent Cultivation Project in Colleges and Universities in Anhui province (grant number gxbjZD46), Natural Science Research Project of Anhui University (grant number KJ2020A0063 and KJ2020A0076).
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Hong, P., Huang, Y., Chen, M. et al. Efficacy of inorganic nitrogen removal by a salt-tolerant aerobic denitrifying bacterium, Pseudomonas sihuiensis LK-618. Bioprocess Biosyst Eng (2021). https://doi.org/10.1007/s00449-021-02525-7
- Aerobic denitrification
- Nitrogen removal
- Nitrogen balance
- Salt tolerance