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Efficacy of inorganic nitrogen removal by a salt-tolerant aerobic denitrifying bacterium, Pseudomonas sihuiensis LK-618

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Abstract

An aerobic denitrifying bacterium, stain LK-618, was isolated from lake sediment surface and the efficacy of inorganic nitrogen removal was tested. Stain LK-618 identified as Pseudomonas sihuiensis by 16S rRNA sequencing analysis. Trisodium citrate was found to be the ideal carbon source for this strain. When an initial nitrogen sources of approximately 50 mg/L nitrate, ammonium, or nitrite was solely selected as the nitrogen source, the nitrogen removal efficiencies were 91.4% (3.86 mg/L/h), 95.07% (2.47 mg/L/h) and 97.7% (2.41 mg/L/h), respectively. Nitrogen balance analysis revealed that 55.12% NO3−-N was removed as N2. Response surface methodology (RSM) analysis demonstrated that the optimal Total Nitrogen (TN) removal ratio for strain LK-618 was under C/N ratio of 12.63, shaking speed of 52.06 rpm, temperature of 28.5 °C and pH of 6.86. In addition, strain LK-618 could tolerate NaCl concentrations up to 20 g/L, and its most efficient denitrification capacity was presented at NaCl concentrations of 0–10 g/L. Therefore, strain LK-618 has potential application on the removal of inorganic nitrogen from saline wastewater under aerobic conditions.

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Fig. 1
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source on cell growth and NO3−-N removal efficiency by strain LK-618

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (grant number 31670465, 31800112, 31800390 and 31870450), Advanced Talent Cultivation Project in Colleges and Universities in Anhui province (grant number gxbjZD46), Natural Science Research Project of Anhui University (grant number KJ2020A0063 and KJ2020A0076).

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Authors and Affiliations

  1. Collaborative Innovation Center of Recovery and Reconstruction of Degraded Ecosystem in Wanjiang Basin Co-Founded By Anhui Province and Ministry of Education, School of Ecology and Environment, Anhui Normal University, Wuhu, China

    Pei Hong

  2. Key Laboratory of Algal Biology of the Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, No. 7 Donghu South Road, Wuhan, 430072, China

    Pei Hong & Bangding Xiao

  3. Key Laboratory for the Conservation and Utilization of Important Biological Resources of Anhui Province, College of Life Sciences, Anhui Normal University, Wuhu, 241000, China

    Yanlin Huang & Minglin Chen

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  1. Pei Hong
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Correspondence to Bangding Xiao.

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Hong, P., Huang, Y., Chen, M. et al. Efficacy of inorganic nitrogen removal by a salt-tolerant aerobic denitrifying bacterium, Pseudomonas sihuiensis LK-618. Bioprocess Biosyst Eng 44, 1227–1235 (2021). https://doi.org/10.1007/s00449-021-02525-7

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