Preparation and characterization of chitosan/Nano-ZnO composite film with antimicrobial activity


Chitosan is promising material for making food packaging film with antimicrobial activity. However, chitosan film usually has limited mechanical and antimicrobial properties and higher water solubility. To improve the performance of chitosan film, in this work, chitosan composite films were prepared by incorporating different sizes of zinc oxide particles of 5 μm, 100 nm, and 50 nm. The films were characterized by scanning electron microscopy, X-ray diffraction, Fourier-transform infrared spectroscopy, and mechanical analysis. Antimicrobial assay of the chitosan and CTS/nano-ZnO composite films against Escherichia coli and Staphylococcus aureus show that the composite chitosan films have better antibacterial activity. The film containing 0.3% of 50 nm zinc oxide particles showed the best inhibition rate, suggesting that smaller sizes of nano-ZnO particles have better bacteriostatic activity and potent application as an antibacterial additive ingredient.

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This research was funded by the National Key Research & Development (R&D) Program of China (Grant No. 2016YFD0200502), the National Natural Science Foundation of China (Grant No. 31772193), the Open Research Fund from State Key Laboratory for Biology of Plant Diseases and Insect Pests (Grant No. SKLOF202006), and the Fundamental Research Funds for the Central Universities (Grant No. DUT19TD38). We thank LetPub ( for its linguistic assistance during the preparation of this manuscript.

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Correspondence to Jun Yang or Qing Yang.

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Zhang, X., Zhang, Z., Wu, W. et al. Preparation and characterization of chitosan/Nano-ZnO composite film with antimicrobial activity. Bioprocess Biosyst Eng (2021).

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  • Nano-ZnO
  • Chitosan
  • Composite films
  • Antibacterial activity