Design, construction and optimization a flexible bench-scale rotating biological contactor (RBC) for enhanced production of bacterial cellulose by Acetobacter Xylinium


In this research a bench scale rotating biological contactor (RBC) was designed and constructed to produce BC. The effects of variables including rotation speed of the disk, distance between disks, disk type and external aeration on BC productivity were investigated. Results showed that the highest weight of BC produced on the surface of integrated polyethylene discs which rotated at 13 rpm. It was also found that the highest amount of BC was obtained when the space between two adjacent discs was adjusted to 1 cm and the disk number was 16. An aquarium pump was used to investigate the impact of aeration on RBC made of 12 integrated polyethylene discs and operated at optimal rotation speed of 13 rpm. Disk spacing distance was adjusted to 1.5 cm to consider the possible increasing of the thickness of BC film by aeration. Wet weight and dry weight of BC resulted from aerated fermentation increased more than 64 and 47%, respectively as compared to non-aerated RBC. In comparison with static culture, wet weight and dry weight of BC produced in aerated RBC fermentation increased more than 90.7 and 71%, respectively. Nanoscale structure of produced bacterial cellulose was confirmed by SEM analysis.

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  1. 1.

    Hu W, Chen S, Yang J, Li Z, Wang H (2014) Functionalized bacterial cellulose derivatives and nanocomposites. Carbohydr Polym 101:1043–1060

    CAS  Article  Google Scholar 

  2. 2.

    Wahid F, Hu X-H, Chu L-Q, Jia S-R, Xie Y-Y, Zhong C (2018) Development of bacterial cellulose/chitosan based semi-interpenetrating hydrogels with improved mechanical and antibacterial properties. Int J Biol Macromol 122:380–387

    Article  Google Scholar 

  3. 3.

    Blanco A, Monte MC, Campano C, Balea A, Merayo N, Negro C (2018) Chapter 5-Nanocellulose for industrial use: cellulose nanofibers (CNF), cellulose nanocrystals (CNC), and bacterial cellulose (BC). In: Mustansar Hussain C (ed) Handbook of nanomaterials for industrial applications. Elsevier, USA, pp 74–126

    Google Scholar 

  4. 4.

    Chawla P, Bajaj I, Survase S, Singhal R (2009) Microbial cellulose: fermentative production and applications. Food Technol Biotechnol 47:107–124

    CAS  Google Scholar 

  5. 5.

    Reddy TRK, Kim H, Park J-W (2016) Renewable biocomposite properties and their applications. In: Poletto M (ed) Composites from renewable and sustainable materials. IntechOpen, pp 177–197.

  6. 6.

    Abeer MM, Mohd Amin MCI, Martin C (2014) A review of bacterial cellulose-based drug delivery systems: their biochemistry, current approaches and future prospects. J Pharm Pharmacol 66(8):1047–1061

    CAS  Article  Google Scholar 

  7. 7.

    Khattak WA, Khan T, Ul-Islam M, Ullah MW, Khan S, Wahid F, Park JK (2015) Production, characterization and biological features of bacterial cellulose from scum obtained during preparation of sugarcane jaggery (gur). J Food Sci Technol 52(12):8343–8349

    CAS  Article  Google Scholar 

  8. 8.

    Bae SO, Sugano Y, Ohi K, Shoda M (2004) Features of bacterial cellulose synthesis in a mutant generated by disruption of the diguanylate cyclase 1 gene of Acetobacter xylinum BPR 2001. Appl Microbiol Biotechnol 65(3):315–322

    CAS  Article  Google Scholar 

  9. 9.

    Nguyen VT, Flanagan B, Gidley MJ, Dykes GA (2008) Characterization of cellulose production by a Gluconacetobacter xylinus strain from kombucha. Curr Microbiol 57(5):449–453

    CAS  Article  Google Scholar 

  10. 10.

    Son H-J, Kim H-G, Kim K-K, Kim H-S, Kim Y-G, Lee S-J (2003) Increased production of bacterial cellulose by Acetobacter sp. V6 in synthetic media under shaking culture conditions. Bioresour Technol 86(3):215–219

    Article  Google Scholar 

  11. 11.

    Kongruang S (2008) Bacterial cellulose production by Acetobacter xylinum strains from agricultural waste products. Appl Biochem Biotechnol 148(1):245–256

    CAS  Article  Google Scholar 

  12. 12.

    Noro N, Sugano Y, Shoda M (2004) Utilization of the buffering capacity of corn steep liquor in bacterial cellulose production by Acetobacter xylinum. Appl Microbiol Biotechnol 64:199–205

    CAS  Article  Google Scholar 

  13. 13.

    Pacheco G, Nogueira CR, Meneguin AB, Trovatti E, Silva MCC, Machado RTA, Ribeiro SJL, da Silva Filho EC, da S. Barud H, (2017) Development and characterization of bacterial cellulose produced by cashew tree residues as alternative carbon source. Ind Crops Prod 107:13–19

    CAS  Article  Google Scholar 

  14. 14.

    Çakar F, Katı A, Özer I, Demirbağ DD, Şahin F, Aytekin AÖ (2014) Newly developed medium and strategy for bacterial cellulose production. Biochem Eng J 92:35–40

    Article  Google Scholar 

  15. 15.

    Islam MU, Ullah MW, Khan S, Shah N, Park JK (2017) Strategies for cost-effective and enhanced production of bacterial cellulose. Int J Biol Macromol 102:1166–1173

    Article  Google Scholar 

  16. 16.

    Kim Y-J, Kim J-N, Wee Y-J, Park D-H, Ryu H-W (2007) Bacterial cellulose production by Gluconacetobacter sp. RKY5 in a rotary biofilm contactor. Appl Biochem Biotechnol 136–140:529–537

    Google Scholar 

  17. 17.

    Sharma C, Bhardwaj NK (2019) Bacterial nanocellulose: present status, biomedical applications and future perspectives. Mater Sci Eng C 104:109963

    CAS  Article  Google Scholar 

  18. 18.

    Najafpour GD, Zinatizadeh AAL, Lee LK (2006) Performance of a three-stage aerobic RBC reactor in food canning wastewater treatment. Biochem Eng J 30(3):297–302

    CAS  Article  Google Scholar 

  19. 19.

    Padhi SK, Gokhale S (2014) Biological oxidation of gaseous VOCs–rotating biological contactor a promising and eco-friendly technique. J Environ Chem Eng 2(4):2085–2102

    CAS  Article  Google Scholar 

  20. 20.

    Mohammadkazemi F, Doosthoseini K, Azin M (2015) Effect of ethanol and medium on bacterial cellulose production from Gluconacetobacter xylinus PTCC 1734. Cellul Chem Technol.

  21. 21.

    Krystynowicz A, Czaja W, Wiktorowska-Jezierska A, Gonçalves-Miśkiewicz M, Turkiewicz M, Bielecki S (2002) Factors affecting the yield and properties of bacterial cellulose. J Ind Microbiol Biotechnol 29(4):189–195

    CAS  Article  Google Scholar 

  22. 22.

    Bungay H, Serafica G (1999) Production of microbial cellulose using a rotating disk film bioreactor.

  23. 23.

    Keluskar RP, Ghosh S, Mani MK, Nayak BB (2019) Application of a rotating biological contactor and moving bed biofilm reactor hybrid in Bioremediating Surimi processing wastewater. Proc Natl Acad Sci, India, Sect B 89(4):1471–1478

    CAS  Article  Google Scholar 

  24. 24.

    Bicelli LG, Augusto MR, Giordani A, Contrera RC, Souza TSO (2020) Intermittent rotation as an innovative strategy for achieving Nitritation in rotating biological contactors. Sci Total Environ 736:139675

    CAS  Article  Google Scholar 

  25. 25.

    Tsouko E, Maina S, Ladakis D, Kookos IK, Koutinas A (2020) Integrated biorefinery development for the extraction of value-added components and bacterial cellulose production from orange peel waste streams. Renewable Energy 160:944–954

    CAS  Article  Google Scholar 

  26. 26.

    Liu M, Zhong C, Wu X-Y, Wei Y-Q, Bo T, Han P-P, Jia S-R (2015) Metabolomic profiling coupled with metabolic network reveals differences in Gluconacetobacter xylinus from static and agitated cultures. Biochem Eng J 101:85–98

    CAS  Article  Google Scholar 

  27. 27.

    Tantratian S, Tammarate P, Krusong W, Bhattarakosol P, Phunsri A (2005) Effect of dissolved oxygen on cellulose production by Acetobacter sp. J Sci Res Chula Univ 30(2):179–186

    CAS  Google Scholar 

  28. 28.

    Kouda T, Naritomi T, Yano H, Yoshinaga F (1997) Effects of oxygen and carbon dioxide pressures on bacterial cellulose production by Acetobacter in aerated and agitated culture. J Ferment Bioeng 84(2):124–127

    CAS  Article  Google Scholar 

  29. 29.

    Hwang JW, Yang YK, Hwang JK, Pyun YR, Kim YS (1999) Effects of pH and dissolved oxygen on cellulose production by Acetobacter xylinum BRC5 in agitated culture. J Biosci Bioeng 88(2):183–188

    CAS  Article  Google Scholar 

  30. 30.

    Pa’e N, Zahan K, Muhamad I (2011) Production of biopolymer from Acetobacter xylinum using different fermentation methods. Int J Eng Technol 11:90–97

    Google Scholar 

  31. 31.

    Najafpour G, Yieng HA, Younesi H, Zinatizadeh A (2005) Effect of organic loading on performance of rotating biological contactors using Palm Oil Mill effluents. Process Biochem 40(8):2879–2884

    CAS  Article  Google Scholar 

  32. 32.

    Alnnasouri M, Lemaitre C, Gentric C, Dagot C, Pons M-N (2011) Influence of surface topography on biofilm development: experiment and modeling. Biochem Eng J 57:38–45

    CAS  Article  Google Scholar 

  33. 33.

    Cortez S, Teixeira P, Oliveira R, Mota M (2013) Bioreactors: rotating biological contactors. In: Flickinger MC (ed) Upstream industrial biotechnology: equipment, process design, sensing, control, and cGMP operations, vol 2, 1st edn. John Wiley & Sons, Inc., pp 1013–1030

  34. 34.

    Radwan KH, Ramanujam TK (1997) Studies on organic removal of 2,4-dichlorophenol wastewaters using a modified RBC. Bioprocess Eng 16(4):219–223

    CAS  Article  Google Scholar 

  35. 35.

    Guimarães C, Porto P, Oliveira R, Mota M (2005) Continuous decolourization of a sugar refinery wastewater in a modified rotating biological contactor with Phanerochaete chrysosporium immobilized on polyurethane foam disks. Process Biochem 40(2):535–540

    Article  Google Scholar 

  36. 36.

    Tawfik A, Klapwijk A (2010) Polyurethane rotating disc system for post-treatment of anaerobically pre-treated sewage. J Environ Manage 91(5):1183–1192

    CAS  Article  Google Scholar 

  37. 37.

    Serafica G, Mormino R, Bungay H (2002) Inclusion of solid particles in bacterial cellulose. Appl Microbiol Biotechnol 58(6):756–760

    CAS  Article  Google Scholar 

  38. 38.

    Kumar A, Rao KM, Kwon SE, Lee YN, Han SS (2017) Xanthan gum/bioactive silica glass hybrid scaffolds reinforced with cellulose nanocrystals: Morphological, mechanical and in vitro cytocompatibility study. Mater Lett 193:274–278

    CAS  Article  Google Scholar 

  39. 39.

    Ostadhossein F, Mahmoudi N, Morales-Cid G, Tamjid E, Navas F, Soriano-Cuadrado B, Manuel J, López Paniza JM, Simchi A (2015) Development of chitosan/bacterial cellulose composite films containing nanodiamonds as a potential flexible platform for wound dressing. Materials 8:6401–6418

    CAS  Article  Google Scholar 

  40. 40.

    Saini S, Belgacem MN, Bras J (2017) Effect of variable aminoalkyl chains on chemical grafting of cellulose nanofiber and their antimicrobial activity. Mater Sci Eng C 75:760–768

    CAS  Article  Google Scholar 

  41. 41.

    Rachini A, Le Troedec M, Peyratout C, Smith A (2012) Chemical modification of hemp fibers by silane coupling agents. J Appl Polym Sci 123(1):601–610

    CAS  Article  Google Scholar 

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This work was financed by Department of Bioscience and biotechnology, Faculty of Chemistry and chemical engineering, Malek Ashtar university of Technology, Tehran, Iran.

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Correspondence to Valiollah Babaeipour.

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soleimani, A., Hamedi, S., Babaeipour, V. et al. Design, construction and optimization a flexible bench-scale rotating biological contactor (RBC) for enhanced production of bacterial cellulose by Acetobacter Xylinium. Bioprocess Biosyst Eng (2021).

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  • Acetobacter xylinium
  • Bacterial nano-cellulose
  • Productivity
  • Rotating biological contactor (RBC)