Improvement of cadaverine production in whole cell system with baker’s yeast for cofactor regeneration

Abstract

Cadaverine, 1,5-diaminopentane, is one of the most promising chemicals for biobased-polyamide production and it has been successfully produced up to molar concentration. Pyridoxal 5′-phosphate (PLP) is a critical cofactor for inducible lysine decarboxylase (CadA) and is required up to micromolar concentration level. Previously the regeneration of PLP in cadaverine bioconversion has been studied and salvage pathway pyridoxal kinase (PdxY) was successfully introduced; however, this system also required a continuous supply of adenosine 5′-triphosphate (ATP) for PLP regeneration from pyridoxal (PL) which add in cost. Herein, to improve the process further a method of ATP regeneration was established by applying baker’s yeast with jhAY strain harboring CadA and PdxY, and demonstrated that providing a moderate amount of adenosine 5′-triphosphate (ATP) with the simple addition of baker’s yeast could increase cadaverine production dramatically. After optimization of reaction conditions, such as PL, adenosine 5′-diphosphate, MgCl2, and phosphate buffer, we able to achieve high production (1740 mM, 87% yield) from 2 M l-lysine. Moreover, this approach could give averaged 80.4% of cadaverine yield after three times reactions with baker’s yeast and jhAY strain. It is expected that baker’s yeast could be applied to other reactions requiring an ATP regeneration system.

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Acknowledgements

This study was supported by the National Research Foundation of Korea (NRF) (NRF-2019R1F1A1058805 and NRF-2019M3E6A1103979), and the Research Program to solve the social issues of the NRF funded by the Ministry of Science and ICT (2017M3A9E4077234). This work was also supported by R&D Program of MOTIE/KEIT (20009508).

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Correspondence to Yung-Hun Yang.

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Han, YH., Kim, H.J., Choi, TR. et al. Improvement of cadaverine production in whole cell system with baker’s yeast for cofactor regeneration. Bioprocess Biosyst Eng (2021). https://doi.org/10.1007/s00449-020-02497-0

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Keywords

  • Bioproduction
  • Cadaverine
  • Pyridoxal 5′-phosphate
  • Adenosine 5′-triphosphate
  • Baker’s yeast