Green and efficient biosynthesis of pectin-based copper nanoparticles and their antimicrobial activities


Herein, we reported a green biosynthesis method of copper nanoparticles (CuNPs) at microwave irradiation condition by using pectin as a stabilizer and ascorbic acid as a reducing agent. Under the optimum conditions, CuNPs1 and 2 were synthesized under microwave times 0 and 3 min, respectively. Transmission electron microscope and scanning electron microscope (SEM) tests showed that CuNPs1 and 2 had irregular polygon particles with average diameters of 61.9 ± 19.4 and 40.9 ± 13.6 nm, respectively. Zeta potentials of CuNPs1 and 2 were −45.2 and −48.7 mV, respectively. X-ray diffraction, Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), and X-ray photoelectron spectroscopy techniques were used to characterize the properties of CuNPs. Furthermore, inhibition zone tests showed that CuNPs2 exhibited higher antimicrobial activities against Escherichia coli, Staphylococcus aureus, and Aspergillus japonicus than CuNPs1. The antibacterial activities were also studied by the bacterial growth kinetics in broth media, and CuNPs2 exhibited lower minimum bactericidal concentrations than CuNPs1.

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  1. 1.

    Suarez-Cerda J, Espinoza-Gomez H, Alonso-Nunez G, Rivero IA, Gochi-Ponce Y, Flores-Lopez LZ (2017) A green synthesis of copper nanoparticles using native cyclodextrins as stabilizing agents. J Saudi Chem Soc 21:341–348

    CAS  Article  Google Scholar 

  2. 2.

    Musa A, Ahmad MB, Hussein MZ, Saiman MI, Sani HA (2017) Preparation, characterization and catalytic activity of biomaterial-supported copper nanoparticles. Res Chem Intermediat 43:801–815

    CAS  Article  Google Scholar 

  3. 3.

    Dong C, Cai H, Zhang X, Cao C (2014) Synthesis and characterization of monodisperse copper nanoparticles using gum acacia. Physica E 57:12–20

    CAS  Article  Google Scholar 

  4. 4.

    Zain NM, Stapley AGF, Shama G (2014) Green synthesis of silver and copper nanoparticles using ascorbic acid and chitosan for antimicrobial applications. Carbohydr Polym 112:195–202

    CAS  Article  Google Scholar 

  5. 5.

    Keihan AH, Veisi H, Veasi H (2017) Green synthesis and characterization of spherical copper nanoparticles as organometallic antibacterial agent. Appl Organomet Chem 31:e3642

    Article  Google Scholar 

  6. 6.

    Venkatakrishnan S, Veerappan G, Elamparuthi E, Veerappan A (2014) Aerobic synthesis of biocompatible copper nanoparticles: promising antibacterial agent and catalyst for nitroaromatic reduction and C–N cross coupling reaction. Rsc Adv 4:15003–15006

    CAS  Article  Google Scholar 

  7. 7.

    Bashir O, Hussain S, Al-Thabaiti SA, Khan Z (2015) Synthesis, optical properties, stability, and encapsulation of Cu-nanoparticles. Spectrochim Acta Part A 140:265–273

    CAS  Article  Google Scholar 

  8. 8.

    Zahran MK, Ahmed HB, El-Rafie MH (2014) Facile size-regulated synthesis of silver nanoparticles using pectin. Carbohydr Polym 111:971–978

    CAS  Article  Google Scholar 

  9. 9.

    Zhang W, Zhao XJ, Jiang Y, Zhou Z (2017) Citrus pectin derived silver nanoparticles and their antibacterial activity. Inorg Nano Met Chem 47:15–20

    CAS  Article  Google Scholar 

  10. 10.

    Su D-l, Li P-j, Ning M, Li G-y, Shan Y (2019) Microwave assisted green synthesis of pectin based silver nanoparticles and their antibacterial and antifungal activities. Mater Lett 244:35–38

    CAS  Article  Google Scholar 

  11. 11.

    Nadezhda VI, Natalya NT, Lydmila AEK, Vasilyi AB (2012) The study of the reaction of pectin-Ag(0) nanocomposites formation. Int J Carbohydr Chem 2012:ID459410

    Google Scholar 

  12. 12.

    Li BJ, Li YY, Wu YH, Zhao YB (2014) Synthesis of water-soluble Cu/PAA composite flowers and their antibacterial activities. Mat Sci Eng C-Mater 35:205–211

    Article  Google Scholar 

  13. 13.

    Baranwal A, Srivastava A, Kumar P, Bajpai VK, Maurya PK, Chandra P (2018) Prospects of nanostructure materials and their composites as antimicrobial agents. Front Microbiol 9:422

    Article  Google Scholar 

  14. 14.

    Chen J, Wang J, Zhang X, Jin Y (2008) Microwave-assisted green synthesis of silver nanoparticles by carboxymethyl cellulose sodium and silver nitrate. Mater Chem Phys 108:421–424

    CAS  Article  Google Scholar 

  15. 15.

    Ngoan Thi N, Binh Hai N, Duong Thi B, Dien Gia P, Van Tran K, Loc Thai N, Lam Dai T (2014) Microwave-assisted synthesis of silver nanoparticles using chitosan: a novel approach. Mater Manuf Process 29:418–421

    Article  Google Scholar 

  16. 16.

    Kahrilas GA, Wally LM, Fredrick SJ, Hiskey M, Prieto AL, Owens JE (2014) Microwave-assisted green synthesis of silver nanoparticles using orange peel extract. ACS Sustain Chem Eng 2:367–376

    CAS  Article  Google Scholar 

  17. 17.

    Rufus SNA, Philip D (2016) Microwave-assisted rapid synthesis of copper nanoparticles with exceptional stability and their multifaceted applications. J Mol Liq 221:1008–1021

    Article  Google Scholar 

  18. 18.

    Li P-j, Xia J-l, Nie Z-y, Shan Y (2016) Pectic oligosaccharides hydrolyzed from orange peel by fungal multi enzyme complexes and their prebiotic and antibacterial potentials. LWT Food Sci Technol 69:203–210

    CAS  Article  Google Scholar 

  19. 19.

    Ahmed HB, Zahran MK, Emam HE (2016) Heatless synthesis of well dispersible Au nanoparticles using pectin biopolymer. Int J Biol Macromol 91:208–219

    CAS  Article  Google Scholar 

  20. 20.

    Hanaor D, Michelazzi M, Leonelli C, Sorrell CC (2012) The effects of carboxylic acids on the aqueous dispersion and electrophoretic deposition of ZrO2. J Eur Ceram Soc 32:235–244

    CAS  Article  Google Scholar 

  21. 21.

    Lv Q, Zhang B, Xing X, Zhao Y, Cai R, Wang W, Gu Q (2018) Biosynthesis of copper nanoparticles using Shewanella loihica PV-4 with antibacterial activity: novel approach and mechanisms investigation. J Hazard Mater 347:141–149

    CAS  Article  Google Scholar 

  22. 22.

    Wang Z, Chen M, Shu J, Li Y (2016) One-step solvothermal synthesis of Fe3O4@Cu@Cu2O nanocomposite as magnetically recyclable mimetic peroxidase. J Alloy Compd 682:432–440

    CAS  Article  Google Scholar 

  23. 23.

    Usman MS, El Zowalaty ME, Shameli K, Zainuddin N, Salama M, Ibrahim NA (2013) Synthesis, characterization, and antimicrobial properties of copper nanoparticles. Int J Nanomed 8:4467–4478

    Google Scholar 

  24. 24.

    Jomova K, Baros S, Valko M (2012) Redox active metal-induced oxidative stress in biological systems. Transit Metal Chem 37:127–134

    CAS  Article  Google Scholar 

  25. 25.

    Mishra S, Singh BR, Singh A, Keswani C, Naqvi AH, Singh HB (2014) Biofabricated silver nanoparticles act as a strong fungicide against bipolaris sorokiniana causing spot blotch disease in wheat. PLoS ONE 9:e97881

    Article  Google Scholar 

  26. 26.

    Marambio-Jones C, Hoek EV (2010) A review of the antibacterial effects of silver nanomaterials and potential implications for human health and the environment. J Nanopart Res 12:1531–1551

    CAS  Article  Google Scholar 

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This study was financially supported by a grant from the Middle-aged and Young Teachers’ Basic Ability Promotion Project of Guangxi (No. 2018KY0257), the Research Program of Guangxi Specially-invited Experts (Ting Fa [2018] 39th), the Guangxi Key Laboratory of Electrochemical and Magneto-chemical Functional Materials (EMFM 20162204), and Guangxi Science and Technology Base and Specialized Talents (AD19110074). The authors appreciate Ms. Liao Li-qiong for editing the figures in this manuscript.

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Correspondence to Pei-jun Li or Yang Shan.

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Li, P., Liang, J., Su, D. et al. Green and efficient biosynthesis of pectin-based copper nanoparticles and their antimicrobial activities. Bioprocess Biosyst Eng (2020).

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  • Copper nanoparticles
  • Microwave
  • Pectin
  • Antimicrobial activities