Removal of nitrogen by heterotrophic nitrification–aerobic denitrification of a novel halotolerant bacterium Pseudomonas mendocina TJPU04

Abstract

Excess inorganic nitrogen in water poses a severe threat to enviroment. Removal of inorganic nitrogen by heterotrophic nitrifying–aerobic denitrifying microorganism is supposed to be a promising and applicable technology only if the removal rate can be maintained sufficiently high in real wastewater under various conditions, such as high concentration of salt and wide range of different nitrogen concentrations. Here, a new heterotrophic nitrifying–aerobic denitrifying bacterium was isolated and named as Pseudomonas mendocina TJPU04, which removes NH4+-N, NO3-N and NO2-N with average rate of 4.69, 5.60, 4.99 mg/L/h, respectively. It also maintains high nitrogen removal efficiency over a wide range of nitrogen concentrations. When concentration of NH4+-N, NO3-N and NO2-N was up to 150, 150 and 50 mg/L, 98%, 93%, and 100% removal efficiency could be obtained, respectively, after 30-h incubation under sterile condition. When it was applied under non-sterile condition, the ammonia removal efficiency was slightly lower than that under sterile condition. However, the nitrate and nitrite removal efficiencies under non-sterile condition were significantly higher than those under sterile condition. Strain TJPU04 also showed efficient nitrogen removal performance in the presence of high concentration of salt and nitrogen. In addition, the removal efficiencies of NH4+-N, NO3-N and TN in real wastewater were 91%, 52%, and 75%, respectively. These results suggest that strain TJPU04 is a promising candidate for efficient removal of inorganic nitrogen in wastewater treatment.

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Acknowledgements

This work was supported by Natural Science Foundation of Tianjin (no. 16JCYBJC23800), National Science Foundation (no. 31872388), Science and Technology Correspondent Project of Tianjin (no. 18JCTPJC55400) and The Science and Technology Plans of Tianjin (no. 17PTSYJC00040 and 18PTSYJC00180).

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Correspondence to Xiaoling He or Dongsheng Wei.

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He, X., Sun, Q., Xu, T. et al. Removal of nitrogen by heterotrophic nitrification–aerobic denitrification of a novel halotolerant bacterium Pseudomonas mendocina TJPU04. Bioprocess Biosyst Eng 42, 853–866 (2019). https://doi.org/10.1007/s00449-019-02088-8

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Keywords

  • Pseudomonas mendocina TJPU04
  • Simultaneous nitrification and denitrification
  • Nitrogen removal
  • Salt tolerance
  • Wastewater treatment