Production of cutinase by solid-state fermentation and its use as adjuvant in bioherbicide formulation

Abstract

In the present study, it was presented a strategy to maximize the cutinase production by solid-state fermentation from different microorganisms and substrates. The best results were observed using Fusarium verticillioides, rice bran being the main substrate. Maximum yield of cutinase obtained by the strain was 16.22 U/g. For concentration, ethanol precipitation was used, and the purification factor was 2.4. The optimum temperature and pH for enzyme activity were 35 °C and 6.5, respectively. The enzyme was stable at a wide range of temperature and at all pH values tested. The concentrated cutinase was used as an adjuvant in a formulation containing cutinase + bioherbicide. The use of enzyme increased the efficiency of bioherbicide, since cutinase was responsible to remove/degrade the cutin that recovery the weed leaves and difficult the bioherbicide absorption. Cutinase showed to be a promising product to be used in formulation of bioherbicides.

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Acknowledgements

The authors thank CAPES for the scholarships as well as CNPq for the financial support of this work.

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Correspondence to Marcio A. Mazutti.

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de Oliveira, C.T., Alves, E.A., Todero, I. et al. Production of cutinase by solid-state fermentation and its use as adjuvant in bioherbicide formulation. Bioprocess Biosyst Eng 42, 829–838 (2019). https://doi.org/10.1007/s00449-019-02086-w

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Keywords

  • Agroindustrial residues
  • Ethanol precipitation
  • Enzyme characterization
  • Bioherbicide action