Advertisement

Bioprocess and Biosystems Engineering

, Volume 41, Issue 7, pp 1061–1071 | Cite as

Combined production of fucoxanthin and EPA from two diatom strains Phaeodactylum tricornutum and Cylindrotheca fusiformis cultures

  • Hui Wang
  • Yan Zhang
  • Lin Chen
  • Wentao Cheng
  • Tianzhong Liu
Research Paper

Abstract

Fucoxanthin and eicosapentaenoic acid (EPA) provide significant health benefits for human population. Diatom is a potential natural livestock for the combined production of EPA and fucoxanthin. In this study, first, the effects of three important parameters including light intensity, nitrogen concentration and salinity were evaluated for the production of EPA and fucoxanthin in two diatom strains Phaeodactylum tricornutum and Cylindrotheca fusiformis. And then, two steps method based on light intensity were applied to produce EPA and fucoxanthin in large scale. Higher light intensity was first adopted for the high growth rate and lipid content of diatom, and after a period of time, light intensity was lowered to enhance the accumulation of fucoxanthin and EPA. In final, the highest EPA yields were 62.55 and 27.32 mg L−1 for P. tricornutum and C. fusiformis, and the fucoxanthin yield reached 8.32 and 6.05 mg L−1, respectively.

Keywords

Fucoxanthin Eicosapentaenoic acid Diatom P. tricornutum C. fusiformis 

Notes

Acknowledgements

This work was supported by Shandong Provincial Natural Science Foundation, China (ZR2017QC007) and Marine economic innovation and development regional demonstration project of Qingdao.

References

  1. 1.
    Miyashita K, Nishikawa S, Beppu F, Tsukui T, Abe M, Hosokawa M (2011) The allenic carotenoid fucoxanthin, a novel marine nutraceutical from brown seaweeds. J Sci Food Agric 91:1166–1174CrossRefPubMedGoogle Scholar
  2. 2.
    Ulrike E, Alexandros B, Jürgen B, Claudia B, Gerhard S (2016) Limitations in the biosynthesis of fucoxanthin as targets for genetic engineerng in Phaeodactylum tricornutum. J Appl Phycol 28:123–129CrossRefGoogle Scholar
  3. 3.
    Maeda H, Hosokawa M, Sashima T, Murakami-Funayama K, Miyashita K (2009) Anti-obesity and anti-diabetic effects fucoxanthin on diet-induced obesity conditions in a marine model. Mol Med Rep 2:897–902CrossRefPubMedGoogle Scholar
  4. 4.
    Tanaka T, Shnimizu M, Moriwaki H (2012) Cancer chemoprevention by carotenoids. Molecules 17:3202–3242CrossRefPubMedGoogle Scholar
  5. 5.
    Yoshioka H, Ishida M, Nishi K, Oda H, Toyohara H, Sugahara T (2014) Studies on anti-allergic activity of Sargassum horneri extract. J Funct Foods 10:154–160CrossRefGoogle Scholar
  6. 6.
    Mulder KJM, Lamers PP, Martens DE, Wijffels RH (2014) Phototrophic pigment production with microalgae: biological constraints and opportunities. J Phycol 50:229–242CrossRefGoogle Scholar
  7. 7.
    Guo BB, Liu B, Yang B, Sun PP, Lu X, Liu J, Chen F (2016) Screening of diatom strains and characterization of Cyclotella cryptica as a potential fucoxanthin producer. Mar Drugs 14:125CrossRefPubMedCentralGoogle Scholar
  8. 8.
    Lee JH, O’Keefe JH, Lavie CJ, Harris WS (2009) Omega-3 fatty acids: cardiovascular benefits, sources and sustainability. Nat Rev Cardiol 6:753–758CrossRefPubMedGoogle Scholar
  9. 9.
    Gharami K, Das M, Das S (2015) Essential role of docosahexaenoic acid towards development of a smarter brain. Neurochem Int 89:51–62CrossRefPubMedGoogle Scholar
  10. 10.
    Kim SM, Kang SW, Kwon ON, Chung D, Pan CH (2012) Fucoxanthin as a major carotenoid in Isochrysis aff. galbana: characterization of extraction for commercial application. J Korean Soc Appl Biol Chem 55:477–483CrossRefGoogle Scholar
  11. 11.
    Li Y, Horsman M, Wang B, Wu N, Lan CQ (2008) Effects of nitrogen sources on cell growth and lipid accumulation of green alga Neochloris oleoabundans. Appl Microbiol Biotechnol 81:629–636CrossRefPubMedGoogle Scholar
  12. 12.
    Pahl SL, Lewis DM, King KD, Chen F (2012) Heterotrophic growth and nutritional aspects of the diatom Cyclotella cryptica (Bacillariophyceae): effect of nitrogen source and concentration. J Appl Phycol 24:301–307CrossRefGoogle Scholar
  13. 13.
    Alipanah L, Rohloff J, Winge P, Bones AM, Brembu T (2015) Whole-cell response to nitrogen deprivation in the diatom Phaeodactylum tricornutum. J Exp Bot 66:6281–6296CrossRefPubMedPubMedCentralGoogle Scholar
  14. 14.
    Hernandez ML, Padilla MN, Sicardo MD, Mancha M, Martinez-Rivas JM (2011) Effect of different environmental stresses on the expression of oleate desaturase genes and fatty acid composition in olive fruit. Phytochemistry 72:178–187CrossRefPubMedGoogle Scholar
  15. 15.
    Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917CrossRefPubMedGoogle Scholar
  16. 16.
    Chen L, Liu TZ, Zhang W, Chen XL, Wang JF (2012) Biodiesel production from algae oil high in free fatty acids by two-step catalytic conversion. Bioresour Technol 111:208–214CrossRefPubMedGoogle Scholar
  17. 17.
    Breuer G, Lamers PP, Martens DE, Draaisma RB, Wijffesl RH (2013) Effect of light intensity, pH, and temperature on triacylglycerol (TAG) accumulation induced by nitrogen starvation in Scenedesmus obliquus. Bioresour Technol 143:1–9CrossRefPubMedGoogle Scholar
  18. 18.
    Dillschneider R, Steinweg C, Rosello-Sastre R, Posten C (2013) Biofuels from microalgae: photoconversion efficiency during lipid accumulation. Bioresour Technol 142:647–654CrossRefPubMedGoogle Scholar
  19. 19.
    Alma GL, Jorge B, Marco RP (2016) Growth kinetics and fucoxanthin production of Phaeodactylum tricornutum and Isochrysis galbana cultures at different light and agitation conditions. J Appl Phycol 28:849–860CrossRefGoogle Scholar
  20. 20.
    Wu HL, Li T, Wang GH, Dai SK, He H, Xiang WZ (2016) A comparative analysis of fatty acid composition and fucoxanthin content in six Phaeodactylum tricornutum strains from different origins. Chin J Oceanol Limnol 34(2):391–398CrossRefGoogle Scholar
  21. 21.
    Remmers IM, Martens DE, Wijffels RH, Lamers PP (2017) Dynamics of triacylglycerol and EPA production in Phaeodactylum tricornutum under nitrogen starvation at different light intensities. Plos One 12(4):e0175630CrossRefPubMedPubMedCentralGoogle Scholar
  22. 22.
    Xiao X, Si X, Yuan Z, Xu X, Li G (2012) Isolation of fucoxanthin from edible brown algae by microwave-assisted extraction coupled with high-speed countercurrent chromatography. J Sep Sci 35:2313–2317CrossRefPubMedGoogle Scholar
  23. 23.
    Jiang Y, Yoshida T, Ouigg A (2012) Photosynthetic performance, lipid production and biomass composition in response to nitrogen limitation in marine microalgae. Plant Physiol Biochem 54:70–77CrossRefPubMedGoogle Scholar
  24. 24.
    Xia S, Wang K, Wan LL, Li A, Hu Q, Zhang CW (2013) Production, characterization, and antioxidant activity of fucoxanthin from the marine diatom Odontella aurita. Mar Drugs 11:2667–2681CrossRefPubMedPubMedCentralGoogle Scholar
  25. 25.
    Ye L, Jiang XM, Mao XX, Gao XZ, Zhang ZL (2015) Effects of temperature, light intensity and salinity on the growth, total lipid and fatty acid of Phaeodactylum tricornutum mutant. Chin J Ecol 34(2):454–462Google Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Hui Wang
    • 1
    • 2
  • Yan Zhang
    • 1
    • 3
  • Lin Chen
    • 1
    • 2
  • Wentao Cheng
    • 1
    • 2
  • Tianzhong Liu
    • 1
    • 2
  1. 1.CAS Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of SciencesQingdaoPeople’s Republic of China
  2. 2.Shandong Provincial Key Laboratory of Bioenergy Resources, Qingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of SciencesQingdaoPeople’s Republic of China
  3. 3.University of Chinese Academy of SciencesBeijingPeople’s Republic of China

Personalised recommendations