Efficient and low-cost alternative of lipase concentration aiming at the application in the treatment of waste cooking oils
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In this study, we evaluated the concentration of lipases from Aspergillus niger using efficient and low-cost methods aiming at application in the treatment of waste cooking oils. The change in ionic strength of the medium by the addition of salt and precipitation with ethanol increased the specific activity from 2.90 to 28.50 U/mg, resulting in a purification factor of 9.82-fold. The use of acetone resulted in a specific activity of 33.63 U/mg, resulting in a purification factor of 11.60-fold. After that, the concentrated lipase was used in the hydrolysis of waste cooking oil and 753.07 and 421.60 µmol/mL of free fatty acids were obtained for the enzyme precipitated with ethanol and acetone, respectively. The hydrolysis of waste cooking oil catalyzed by homemade purified lipase in ultrasonic media can be considered a pretreatment of oil by converting a significant amount of triglycerides into free fatty acids.
KeywordsHomemade lipases Precipitation Waste cooking oil
CAPES, FAPERGS, and CNPq are acknowledged for financial support.
- 2.Pandey A, Benjamin S, Soccol CR, Nigam P, Krieger N, Thomaz-Soccol V (1999) The realm of microbial lipases in biotechnology. Biotechnol App Biochem 65:252–254Google Scholar
- 4.Ayaz B, Ugur A, Boran R (2015) Purification and characterization of organic solvent tolerant lipases from Streptomyces sp. OC119-7 for biodiesel production. Biocatal Agric Biotechnol 4:103–108Google Scholar
- 10.Martins TS (2001) Produção e purificação de lipases de Yarrowia lipolytica (IMUFRJ 50682), Dissertação de Mestrado. Centro de Ciências da Saúde, Universidade Federal de Rio de Janeiro, Rio de JaneiroGoogle Scholar
- 12.Gupta R, Beg O, Lorenz P (2012) Bacterial alkaline proteases: molecular approaches and industrial applications. Appl Microbiol Biotechnol 59:15–32Google Scholar
- 14.Pinto-Coelho RM (2009) Reciclagem e desenvolvimento sustentável no Brasil. Recóleo Coleta e Reciclagem de Óleos, Belo Horizonte, pp 241–282Google Scholar
- 15.Treichel H, Sbardelotto M, Venturin B, Dall Agnol A, Mulinari J, Golunski SM, Baldoni DB, Bevilacqua CB, Jacques RJS, Vargas GDLP, Mossi AJ (2016) Lipase production from a newly isolated Aspergillus niger by solid state fermentation. Curr Biotechnol 5:1–7Google Scholar
- 20.Mulinari J, Venturin B, Sbardelotto M, Dall Agnol A, Scapini T, Camargo AF, Baldissarelli DP, Modkovski TA, Rossetto V, Dalla Rosa C, Reichert FW Jr, Golunski SM, Vieitez I, Vargas GDLP., Mossi AJ, Treichel H (2017) Ultrasound-assisted hydrolysis of waste cooking oil catalyzed by homemade lipases. Ultrason Sonochem 35:313–318CrossRefPubMedGoogle Scholar
- 21.Freire DM, Teles EM, Bom EP, Sant’Anna GL Jr (1997) Lipase production by Peniillium restrictum in a bench-scale fermenter: effect of carbon and nitrogen nutrition, agitation, and aeration. Appl Biochem Biotech 63:63–65Google Scholar