Qualitative and quantitative spectrometric evaluation of soluble microbial products formation in aerobic granular sludge system treating nitrate wastewater
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In present study, the characteristics of soluble microbial products (SMP) were evaluated in aerobic granular sludge system during denitrification process under different chemical oxygen demand/nitrogen (C/N) ratios. Batch experiment showed that the effluent nitrate (NO3−–N) concentration were 15.24 ± 1.83 and 1.72 ± 1.53 mg/L at C/N ratio of 1 and 6, respectively. For the release of SMP, the protein (PN) and polysaccharide contents increased from 1.23 ± 0.38 and 7.46 ± 1.13 mg/L to 1.80 ± 0.76 and 10.53 ± 1.24 mg/L with increasing C/N ratios, respectively. Excitation–emission matrix identified four peaks in SMP, including aromatic PN-like, tryptophan PN-like, fulvic acid-like and humic acid-like substances. Fluorescence regional integration suggested that biodegradable PN-like substances occupied the percentage between 53.0 and 61.7% in SMP. Synchronous fluorescence spectra coupled with two-dimensional correlation spectroscopy indicated that the release of SMP fractions in the early stage (0–150 min) changed in the following sequences: PN-like fraction > fulvic acid-like fraction.
KeywordsDenitrification Soluble microbial products (SMP) Aerobic granular sludge (AGS) Nitrate (NO3−–N) wastewater Three-dimensional excitation–emission matrix (3D-EEM)
This study was supported by the National Natural Science Foundation of China (21777056), Natural Science Foundation of Shandong Province (ZR201702070162), and QW thanks the Special Foundation for Taishan Scholar Professorship of Shandong Province and UJN (No.ts20130937).
- 22.Di Iaconi C, Ramadori R, Lopez A, Passino R (2007) Aerobic granular sludge systems: the new generation of wastewater treatment technologies. Indengchemres 46:6661–6665Google Scholar
- 26.APHA, AWWA, WEF (2005) Standards methods for the examination of water and wastewater, 21st edn. Am Pub Health Assoc, Washington, DCGoogle Scholar
- 39.Wu N, Wei D, Zhang Y, Xu W, Yan T, Du B, Wei Q (2016) Comparison of soluble microbial products released from activated sludge and aerobic granular sludge systems in the presence of toxic 2,4-dichlorophenol. Bioprocess Biosyst Eng 40:1–10Google Scholar