Growth medium sterilization using decomposition of peracetic acid for more cost-efficient production of omega-3 fatty acids by Aurantiochytrium
- 71 Downloads
Aurantiochytrium can produce significant amounts of omega-3 fatty acids, specifically docosahexaenoic acid and docosapentaenoic acid. Use of a glucose-based medium for heterotrophic growth is needed to achieve a high growth rate and production of abundant lipids. However, heat sterilization for reliable cultivation is not appropriate to heat-sensitive materials and causes a conversion of glucose via browning (Maillard) reactions. Thus, the present study investigated the use of a direct degradation of Peracetic acid (PAA) for omega-3 production by Aurantiochytrium. Polymer-based bioreactor and glucose-containing media were chemically co-sterilized by 0.04% PAA and neutralized through a reaction with ferric ion (III) in HEPES buffer. Mono-cultivation was achieved without the need for washing steps and filtration, thereby avoiding the heat-induced degradation and dehydration of glucose. Use of chemically sterilized and neutralized medium, rather than heat-sterilized medium, led to a twofold faster growth rate and greater productivity of omega-3 fatty acids.
KeywordsAurantiochytrium Chemical sterilization Peracetic acid Omega-3 Maillard reaction
This research was supported by the Basic Science Research Program, through the National Research Foundation of Korea (NRF), and was funded by the Ministry of Science, ICT & Future Planning (2015R1C1A1A01054303). This work was supported by the Fund for New Professor research foundation Program (2015-04-012), Gyeongsang National University, 2015.
- 6.Bhat H, Qazi G, Chopra C (1984) Effect of 5-hydroxymethylfurfural on production of citric acid by Aspergillus niger. Indian J Exp Biol 22:37–38Google Scholar
- 8.Baldry M (1983) The bactericidal, fungicidal and sporicidal properties of hydrogen peroxide and peracetic acid. J Appl Microbiol 54:417–423Google Scholar
- 13.Folch J, Lees M, Sloane-Stanley G (1957) A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226:497–509Google Scholar
- 18.Zhang J, Li J, Tang Y, Xue G (2013) Rapid method for the determination of 5-hydroxymethylfurfural and levulinic acid using a double-wavelength UV spectroscopy. Sci World J 2013:1–6Google Scholar