Preparation of porous hollow Fe3O4/P(GMA–DVB–St) microspheres and application for lipase immobilization
Functional porous hollow microspheres with superparamagnetism, Fe3O4/P(GMA–DVB–St) microspheres, were prepared via a dispersion polymerization based on hollow Fe3O4 microspheres. The resulting hollow microspheres were characterized by means of Fourier-transform infrared spectrophotometer (FT-IR), X-ray diffraction (XRD), transmission electron microscopy (TEM), Brunauer-Emmett-Teller (BET) gas sorptometry, and vibrating sample magnetometer (VSM). It is verified that the resulting hollow microspheres are porous and have high saturation magnetization. For further application, candida rugosa lipase (CRL) was immobilized onto the hollow microshperes, the loading amount of lipase was 143.88 mg CRL/g support and the activity recovery of the obtained immobilized lipase reached 73.25%. Besides, the resulting immobilized CRL (ICRL) were found to have better pH endurance and temperature endurance than the free ones, which showed the optimal catalytic activity with pH of 9.0 and temperature of 60 °C. The ICRL displayed excellent reusability as well.
KeywordsHollow magnetic microspheres Dispersion polymerization Lipase immobilization
This paper is dedicated to the memory of Prof. Yanfeng Li. The author thanks the financial supports from the National Natural Science Foundation of Ningxia (NZ14104), Key Scientific Research Project Foundation of North Minzu University (2015KJ25) and Foundation of Key Laboratory of Powder Materials and Special Ceramics (1405).
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