Quick separation and enzymatic performance improvement of lipase by ionic liquid-modified Fe3O4 carrier immobilization
To promote the activity and stability of immobilized porcine pancreatic lipase (PPL), novel carrier was combined with special immobilization method. Enzymatic activity was enhanced after immobilized onto ionic liquid modified magnetic Fe3O4 by electrostatic adsorption. Activity of immobilized enzyme (PPL-IM/BF4-Fe3O4@CA) reached 596 U/g PPL. Through the combination of electrostatic adsorption and embedding immobilization methods, we improve binding force between the carrier and enzyme, and further enhance the efficiency and stability of immobilized enzyme. The activity of PPL-IM/BF4-Fe3O4@CA after repeated third use was 78%. After storage at room temperature for 5 days, the residual activity was 89%. Enzymatic properties and catalytic kinetics of immobilized enzymes were studied, and the effect mechanism of ionic liquid modified Fe3O4 on PPL was revealed. The effect of ionic liquid on the carrier structure was investigated by characterization of XRD, FT-IR, SEM and TG. The mechanism and enzymatic properties of immobilized PPL via electrostatic adsorption and embedding were analyzed. A novel and efficient immobilized PPL was developed.
KeywordsImmobilization Fe3O4 Ionic liquid Lipase Surface modification
The work was funded by the National Natural Science Foundation of China (no. 21406093), the Natural Science Foundation of Jiangsu province (BK20140529), Key University Science Research Project of Jiangsu Province (14KJB530001), China Postdoctoral Science Foundation (2014M550271), and the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions.
- 9.Tacias-Pascacio VG, Virgen-Ortiz JJ, Jimenez-Perez M, Yates M, Torrestiana-Sanchez B, Rosales-Quintero A, Fernandez-Lafuente R (2017) Evaluation of different lipase biocatalysts in the production of biodiesel from used cooking oil: critical role of the immobilization support. Fuel 200:1–10CrossRefGoogle Scholar
- 14.Kumar-Krishnan S, Hernandez-Rangel A, Pal U, Ceballos-Sanchez O, Flores-Ruiz FJ, Prokhorov E, Arias de Fuentes O, Esparza R, Meyyappan M (2016) Surface functionalized halloysite nanotubes decorated with silver nanoparticles for enzyme immobilization and biosensing. J Mater Chem B 4:2553–2560CrossRefGoogle Scholar
- 22.Rios NS, Pinheiro MP, Santos JCSD., Fonseca TDS, Lima LD, Demattos MC, Freire DMG, Júnior IJDS., Rodríguez-Aguado E, Gonçalves LRB (2016) Strategies of covalent immobilization of a recombinant Candida antarctica lipase B on pore-expanded SBA-15 and its application in the kinetic resolution of (R,S)-phenylethyl acetate. J Mol Catal B Enzym 133:246–258CrossRefGoogle Scholar
- 26.Bonazza HL, Manzo RM, Santos JCSD, Mammarella EJ (2017) Operational and thermal stability analysis of Thermomyces lanuginosus lipase covalently immobilized onto modified chitosan supports. Appl Biochem Biotechnol 47:1–15Google Scholar
- 42.Zhao R, Zhang X, Zheng L, Xu H, Li M (2017) Enantioselective esterification of (R,S)-flurbiprofen catalyzed by lipase in ionic liquid. Green Chem 10:23–28Google Scholar
- 43.Rios N, Pinheiro M, Santos J, Fonseca T, Lima L, Demattos M (2016) Strategies of covalent immobilization of a recombinant Candida antarctica, lipase b on pore-expanded sba-15 and its application in the kinetic resolution of (r,s)-phenylethyl acetate. J Mol Catal B Enzym 133:246–258CrossRefGoogle Scholar
- 44.Aaron Salazar-Leyva J, Lizardi-Mendoza J, Carlos Ramirez-Suarez J, Elena Lugo-Sanchez M, Miriam Valenzuela-Soto E, Marina Ezquerra-Brauer J, Javier Castillo-Yanez F, Pacheco-Aguilar R (2017) Catalytic and operational stability of acidic proteases from monterey sardine (Sardinops sagax caerulea) immobilized on a partially deacetylated chitin support. J Food Biochem 41:41–49Google Scholar
- 45.Palomo JM, Muñoz G, Fernández-Lorente G, Mateo C, Fernández-Lafuente R, Guisán JM (2002) Interfacial adsorption of lipases on very hydrophobic support (octadecyl–Sepabeads): immobilization, hyperactivation and stabilization of the open form of lipases. J Mol Catal B Enzym 19–20:279–286CrossRefGoogle Scholar
- 46.Lage FA, Bassi JJ, Corradini MC, Todero LM, Luiz JH, Mendes AA (2016) Preparation of a biocatalyst via physical adsorption of lipase from Thermomyces lanuginosus on hydrophobic support to catalyze biolubricant synthesis by esterification reaction in a solvent-free system. Enzyme Microb Technol 84:56–64CrossRefGoogle Scholar
- 47.Manoel EA, Ribeiro MFP, Santos JCSD., Coelho MAZ, Simas ABC, Fernandez-Lafuente R, Freire DMG (2015) Accurel MP 1000 as a support for the immobilization of lipase from Burkholderia cepacia: application to the kinetic resolution of myo-inositol derivatives. Process Biochem 50:1557–1564CrossRefGoogle Scholar
- 51.Souza TCD, Fonseca TDS, Costa JAD, Rocha MVP, Mattos MCD, Fernandez-Lafuente R, Gonçalves LRB, Santos JCSD. (2016) Cashew apple bagasse as a support for the immobilization of lipase B from Candida antarctica: application to the chemoenzymatic production of (R)-indanol. J Mol Catal B Enzym 130:58–69CrossRefGoogle Scholar