Bioprocess and Biosystems Engineering

, Volume 41, Issue 5, pp 715–727 | Cite as

Extracellular red Monascus pigment-mediated rapid one-step synthesis of silver nanoparticles and its application in biomedical and environment

  • Sunil H. Koli
  • Bhavana V. Mohite
  • Rahul K. Suryawanshi
  • Hemant P. Borase
  • Satish V. Patil
Research Paper


The development of a safe and eco-friendly method for metal nanoparticle synthesis has an increasing demand, due to emerging environmental and biological harms of hazardous chemicals used in existing nanosynthesis methods. The present investigation reports a rapid one-step, eco-friendly and green approach for the formation of nanosized silver particles (AgNPs) using extracellular non-toxic-colored fungal metabolites (Monascus pigments—MPs). The formation of nanosized silver particles utilizing Monascus pigments was confirmed after exposure of reaction mixture to sunlight, by visually color change and further established by spectrophotometric analysis. The size, shape, and topography of synthesized MPs–AgNPs were well-defined using different microscopic and spectroscopic techniques, i.e., FE-SEM, HR-TEM, and DLS. The average size of MPs–AgNPs was found to be 10–40 nm with a spherical shape which was highly stable and dispersed in the solution. HR-TEM and XRD confirmed crystalline nature of MPs–AgNPs. The biocidal potential of MPs–AgNPs was evaluated against three bacterial pathogens such as Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus and it was observed that the MPs–AgNPs significantly inhibited the growth of all three bacterial pathogens. The anti-biofilm activity of MPs–AgNPs was recorded against antibiotic-resistant P. aeruginosa. Besides, the colorimetric metal sensing using MPs–AgNPs was studied. Among the metals tested, the selective Hg2+-sensing potential at micromolar concentration was observed. In conclusion, this is the rapid one-step (within 12–15 min), environment-friendly method for synthesis of AgNPs and synthesized MPs–AgNPs could be used as a potential antibacterial agent against antibiotic-resistant bacterial pathogens.

Graphical abstract


AgNPs Eco-friendly Rapid synthesis Antibacterial Anti-biofilm Sensing 



Mr. Sunil H. Koli acknowledges to UGC-BSR (University Grants Commission, New Delhi, India) for providing research fellowship (File No.-NMU/SLS/491/2015 UGC-BSR dated 11 August 2015) and also thankful to Research scholar Mr. Manohar Patil and Mr. Chandrashekar Patil (School of Chemical Sciences, NMU Jalgaon) for their kind help. Authors are grateful to Dr. Anil Lachke, Scientist NCL, Pune, India for their constant encouragement. Authors are indebted to University Grants Commission and Department of Science and Technology, India for making the research facilities available under the UGC-SAP and DST-FIST programs sanctioned to the School of Life Sciences.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Supplementary material

449_2018_1905_MOESM1_ESM.docx (248 kb)
Supplementary material 1 (DOCX 247 KB)


  1. 1.
    Ju-Nam Y, Lead JR (2008) Manufactured nanoparticles: an overview of their chemistry, interactions and potential environmental implications. Sci Environ 400:396–414Google Scholar
  2. 2.
    Mohanpuria P, Rana NK, Yadav SK (2008) Biosynthesis of nanoparticles: technological concepts and future applications. J Nanoparticle Res 10:507–517CrossRefGoogle Scholar
  3. 3.
    Choudhary BC, Paul D, Gupta T, Tetgure SR, Garole VJ, Borse AU, Garole DJ (2017) Photocatalytic reduction of organic pollutant under visible light by green route synthesized gold nanoparticles. J Environ Sci 55:236–246CrossRefGoogle Scholar
  4. 4.
    Borase HP, Patil CD, Salunkhe RB, Suryawanshi RK, Salunke BK, Patil SV (2014) Catalytic and synergistic antibacterial potential of green synthesized silver nanoparticles: their ecotoxicological evaluation on Poecillia reticulata. Biotechnol Appl Biochem 61:385–394CrossRefGoogle Scholar
  5. 5.
    Borase HP, Salunkhe RB, Patil CD, Suryawanshi RK, Salunke BK, Wagh ND, Patil SV (2015) Innovative approach for urease inhibition by Ficus carica extract–fabricated silver nanoparticles: an in vitro study. Biotechnol Appl Biochem 62:780–784CrossRefGoogle Scholar
  6. 6.
    Gudikandula K, Charya Maringanti S (2016) Synthesis of silver nanoparticles by chemical and biological methods and their antimicrobial properties. J Exp Nanosci 11:714–721CrossRefGoogle Scholar
  7. 7.
    Li X, Xu H, Chen ZS, Chen G (2011) Biosynthesis of nanoparticles by microorganisms and their applications. J Nanomater 1–16.
  8. 8.
    Borase HP, Salunke BK, Salunkhe RB, Patil CD, Hallsworth JE, Kim BS, Patil SV (2014) Plant extract: a promising biomatrix for ecofriendly, controlled synthesis of silver nanoparticles. Appl Biochem Biotechnol 173:1–29CrossRefGoogle Scholar
  9. 9.
    Kumar PS, Balachandran C, Duraipandiyan V, Ramasamy D, Ignacimuthu S, Al-Dhabi NA (2015) Extracellular biosynthesis of silver nanoparticle using Streptomyces sp. 09 PBT 005 and its antibacterial and cytotoxic properties. Appl Nanosci 5:169–180CrossRefGoogle Scholar
  10. 10.
    Thakkar KN, Mhatre SS, Parikh RY (2010) Biological synthesis of metallic nanoparticles. Nanomed Nanotechnol Biol Med 6:257–262CrossRefGoogle Scholar
  11. 11.
    Borase HP, Patil CD, Suryawanshi RK, Patil SV (2013) Ficus carica latex-mediated synthesis of silver nanoparticles and its application as a chemophotoprotective agent. Appl Biochem Biotechnol 171:676–688CrossRefGoogle Scholar
  12. 12.
    Ravi SS, Christena LR, SaiSubramanian N, Anthony SP (2013) Green synthesized silver nanoparticles for selective colorimetric sensing of Hg2+ in aqueous solution at wide pH range. Analyst 138:4370–4377CrossRefGoogle Scholar
  13. 13.
    Heidarpour F, Ghani WWAK., Fakhru’l-Razi A, Sobri S, Heydarpour V, Zargar M, Mozafari MR (2011) Complete removal of pathogenic bacteria from drinking water using nano silver-coated cylindrical polypropylene filters. Clean Technol Environ Policy 13:499–507CrossRefGoogle Scholar
  14. 14.
    El-Baz AF, El-Batal AI, Abomosalam FM, Tayel AA, Shetaia YM, Yang ST (2016) Extracellular biosynthesis of anti-candida silver nanoparticles using Monascus purpureus. J Microbiol 56:531–540Google Scholar
  15. 15.
    Pantidos N, Horsfall LE (2014) Biological synthesis of metallic nanoparticles by bacteria, fungi and plants. J Nanomed Nanotechnol 5:1CrossRefGoogle Scholar
  16. 16.
    Salunkhe RB, Patil SV, Salunke BK, Patil CD, Sonawane AM (2011) Studies on silver accumulation and nanoparticle synthesis by Cochliobolus lunatus. Appl Biochem Biotechnol 165:221–234CrossRefGoogle Scholar
  17. 17.
    Ahluwalia V, Kumar J, Sisodia R, Shakil NA, Walia S (2014) Green synthesis of silver nanoparticles by Trichoderma harzianum and their bio-efficacy evaluation against Staphylococcus aureus and Klebsiella pneumonia. Ind Crops Prod 55:202–206CrossRefGoogle Scholar
  18. 18.
    El-Sonbaty SM (2013) Fungus-mediated synthesis of silver nanoparticles and evaluation of antitumor activity. Cancer Nanotechnol 4:73–79CrossRefGoogle Scholar
  19. 19.
    Bhainsa KC, D’Souza SF (2006) Extracellular biosynthesis of silver nanoparticles using the fungus Aspergillus fumigatus. Colloids Surf B Biointerfaces 47:160–164CrossRefGoogle Scholar
  20. 20.
    Ingle A, Rai M, Gade A, Bawaskar M (2009) Fusarium solani: a novel biological agent for the extracellular synthesis of silver nanoparticles. J Nanopart Res 11:2079CrossRefGoogle Scholar
  21. 21.
    Ahmad A, Mukherjee P, Senapati S, Mandal D, Khan MI, Kumar R, Sastry M (2003) Extracellular biosynthesis of silver nanoparticles using the fungus Fusarium oxysporum. Colloids Surf B Biointerfaces 28:313–318CrossRefGoogle Scholar
  22. 22.
    Lopes FC, Tichota DM, Pereira JQ, Segalin J, de Oliveira Rios A, Brandelli A (2013) Pigment production by filamentous fungi on agro-industrial byproducts: an eco-friendly alternative. Appl Biochem Biotechnol 171:616–625CrossRefGoogle Scholar
  23. 23.
    Koli SH, Suryawanshi RK, Patil CD, Patil SV (2017) Diversity and applications of versatile pigments produced by Monascus sp. In: Singh OV (eds) Biopigmentation and Bitechnological implementations. Wiley, Hoboken, pp 193–214CrossRefGoogle Scholar
  24. 24.
    Srianta I, Zubaidah E, Estiasih T, Iuchi Y, Yamada M (2017) Antioxidant activity of pigments derived from Monascus purpureus-fermented rice, corn, and sorghum. Int Food Res J 24:1186–1191Google Scholar
  25. 25.
    Puttananjaiah MKH, Dhale MA, Govindaswamy V (2011) Non-toxic effect of Monascus purpureus extract on lactic acid bacteria suggested their application in fermented foods. Food Nutr Sci 2:837CrossRefGoogle Scholar
  26. 26.
    El-Batal AI, El-Baz AF, Abo Mosalam FM, Tayel AA (2013) Gamma irradiation induces silver nanoparticles synthesis by Monascus purpureus. J Chem Pharm Res 5:1–15Google Scholar
  27. 27.
    Koli SH, Mohite BV, Borase HP, Patil SV (2017) Monascus pigments mediated rapid green synthesis and characterization of gold nanoparticles with possible mechanism. J Clust Sci. Google Scholar
  28. 28.
    Koli SH, Suryawanshi RK, Patil CD, Patil SV (2017) Fluconazole treatment enhances extracellular release of red pigments in the fungus Monascus purpureus. FEMS Microbiol Lett 364:fnx058CrossRefGoogle Scholar
  29. 29.
    Hu Z, Zhang X, Wu Z, Qi H, Wang Z (2012) Perstraction of intracellular pigments by submerged cultivation of Monascus in nonionic surfactant micelle aqueous solution. Appl Microbiol Biotechnol 94:81–89CrossRefGoogle Scholar
  30. 30.
    Suryawanshi RK, Patil CD, Koli SH, Hallsworth JE, Patil SV (2017) Antimicrobial activity of prodigiosin is attributable to plasma-membrane damage. Nat Prod Res 31:572–577CrossRefGoogle Scholar
  31. 31.
    Gupta K, Hazarika SN, Saikia D, Namsa ND, Mandal M (2014) One step green synthesis and anti-microbial and anti-biofilm properties of Psidium guajava L. leaf extract-mediated silver nanoparticles. Mater Lett 125:67–70CrossRefGoogle Scholar
  32. 32.
    Tantra R (2016) Nanomaterial characterization: an introduction nanomaterial. Wiley, Oxford, pp 38–41CrossRefGoogle Scholar
  33. 33.
    Augustine R, Kalarikkal N, Thomas S (2014) A facile and rapid method for the black pepper leaf mediated green synthesis of silver nanoparticles and the antimicrobial study. Appl Nanosci 4:809–818CrossRefGoogle Scholar
  34. 34.
    Narasimha G, Praveen B, Mallikarjuna K, Deva Prasad Raju B (2011) Mushrooms (Agaricus bisporus) mediated biosynthesis of sliver nanoparticles, characterization and their antimicrobial activity. Int J Nano Dimens 2:29–36Google Scholar
  35. 35.
    Gaddala B, Nataru S (2015) Synthesis, characterization and evaluation of silver nanoparticles through leaves of Abrus precatorius L.: an important medicinal plant. Appl Nanosci 5:99–104CrossRefGoogle Scholar
  36. 36.
    Singh K, Panghal M, Kadyan S, Chaudhary U, Yadav JP (2014) Antibacterial activity of synthesized silver nanoparticles from Tinospora cordifolia against multi drug resistant strains of Pseudomonas aeruginosa isolated from burn patients. J Nanomed Nanotechnol 5:1Google Scholar
  37. 37.
    Sankaranarayanan A, Munivel G, Karunakaran G, Kadaikunnan S, Alharbi NS, Khaled JM, Kuznetsov D (2017) Green synthesis of silver nanoparticles using Arachis hypogaea (ground nut) root extract for antibacterial and clinical applications. J Clust Sci 28:995–1008CrossRefGoogle Scholar
  38. 38.
    Durairaj R, Amirulhusni AN, Palanisamy NK, Mohd-Zain Z, Ping LJ (2012) Antibacterial effect of silver nanoparticles on multi drug resistant Pseudomonas aeruginosa. World Acad Sci Eng Technol 6:210–213Google Scholar
  39. 39.
    Feng QL, Wu J, Chen GQ, Cui FZ, Kim TN, Kim JO (2000) A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus. J Biomed Mater Res 52:662–668CrossRefGoogle Scholar
  40. 40.
    Li WR, Xie XB, Shi QS, Zeng HY, You-Sheng OY, Chen YB (2010) Antibacterial activity and mechanism of silver nanoparticles on Escherichia coli. Appl Microbiol Biotechnol 85:1115–1122CrossRefGoogle Scholar
  41. 41.
    Yamanaka M, Hara K, Kudo J (2005) Bactericidal actions of a silver ion solution on Escherichia coli, studied by energy-filtering transmission electron microscopy and proteomic analysis. Appl Environ Microbiol 71:7589–7593CrossRefGoogle Scholar
  42. 42.
    Sun DAXI., Courtney HS, Beachey EH (1988) Berberine sulfate blocks adherence of Streptococcus pyogenes to epithelial cells, fibronectin, and hexadecane. Antimicrob Agents Chemother 32:1370–1374CrossRefGoogle Scholar
  43. 43.
    Boening DW (2000) Ecological effects, transport, and fate of mercury: a general review. Chemosphere 40:1335–1351CrossRefGoogle Scholar
  44. 44.
    Holmes P, James KAF, Levy LS (2009) Is low-level environmental mercury exposure of concern to human health? Sci Total Environ 408:171–182CrossRefGoogle Scholar
  45. 45.
    Zahir F, Rizwi SJ, Haq SK, Khan RH (2005) Low dose mercury toxicity and human health. Environ Toxicol Pharmacol 20:351–360CrossRefGoogle Scholar
  46. 46.
    Borase HP, Patil CD, Salunkhe RB, Suryawanshi RK, Salunke BK, Patil SV (2014) Mercury sensing and toxicity studies of novel latex fabricated silver nanoparticles. Bioprocess Biosyst Eng 37:2223–2233CrossRefGoogle Scholar
  47. 47.
    Chen G, Guo Z, Zeng G, Tang L (2015) Fluorescent and colorimetric sensors for environmental mercury detection. Analyst 140:5400–5443CrossRefGoogle Scholar
  48. 48.
    Chansuvarn W, Tuntulani T, Imyim A (2015) Colorimetric detection of mercury(II) based on gold nanoparticles, fluorescent gold nanoclusters and other gold-based nanomaterials. Trends Analyt Chem 65:83–96CrossRefGoogle Scholar
  49. 49.
    Jeevika A, Shankaran DR (2016) Functionalized silver nanoparticles probe for visual colorimetric sensing of mercury. Mater Res Bull 83:48–55CrossRefGoogle Scholar
  50. 50.
    Sumesh E, Bootharaju MS, Pradeep T (2011) A practical silver nanoparticle-based adsorbent for the removal of Hg2+ from water. J Hazard Mater 189:450–457CrossRefGoogle Scholar
  51. 51.
    Vasileva P, Alexandrova T, Karadjova I (2017) Application of starch-stabilized silver nanoparticles as a colorimetric sensor for mercury(II) in 0.005 mol/L nitric acid. J Chem 1–9.
  52. 52.
    Manivannan S, Ramaraj R (2013) Silver nanoparticles embedded in cyclodextrin–silicate composite and their applications in Hg(II) ion and nitrobenzene sensing. Analyst 138:1733–1739CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Sunil H. Koli
    • 1
  • Bhavana V. Mohite
    • 1
  • Rahul K. Suryawanshi
    • 1
  • Hemant P. Borase
    • 1
  • Satish V. Patil
    • 1
    • 2
  1. 1.School of Life SciencesNorth Maharashtra UniversityJalgaonIndia
  2. 2.North Maharashtra Microbial Culture Collection Centre (NMCC)North Maharashtra UniversityJalgaonIndia

Personalised recommendations