Extracellular red Monascus pigment-mediated rapid one-step synthesis of silver nanoparticles and its application in biomedical and environment
The development of a safe and eco-friendly method for metal nanoparticle synthesis has an increasing demand, due to emerging environmental and biological harms of hazardous chemicals used in existing nanosynthesis methods. The present investigation reports a rapid one-step, eco-friendly and green approach for the formation of nanosized silver particles (AgNPs) using extracellular non-toxic-colored fungal metabolites (Monascus pigments—MPs). The formation of nanosized silver particles utilizing Monascus pigments was confirmed after exposure of reaction mixture to sunlight, by visually color change and further established by spectrophotometric analysis. The size, shape, and topography of synthesized MPs–AgNPs were well-defined using different microscopic and spectroscopic techniques, i.e., FE-SEM, HR-TEM, and DLS. The average size of MPs–AgNPs was found to be 10–40 nm with a spherical shape which was highly stable and dispersed in the solution. HR-TEM and XRD confirmed crystalline nature of MPs–AgNPs. The biocidal potential of MPs–AgNPs was evaluated against three bacterial pathogens such as Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus and it was observed that the MPs–AgNPs significantly inhibited the growth of all three bacterial pathogens. The anti-biofilm activity of MPs–AgNPs was recorded against antibiotic-resistant P. aeruginosa. Besides, the colorimetric metal sensing using MPs–AgNPs was studied. Among the metals tested, the selective Hg2+-sensing potential at micromolar concentration was observed. In conclusion, this is the rapid one-step (within 12–15 min), environment-friendly method for synthesis of AgNPs and synthesized MPs–AgNPs could be used as a potential antibacterial agent against antibiotic-resistant bacterial pathogens.
KeywordsAgNPs Eco-friendly Rapid synthesis Antibacterial Anti-biofilm Sensing
Mr. Sunil H. Koli acknowledges to UGC-BSR (University Grants Commission, New Delhi, India) for providing research fellowship (File No.-NMU/SLS/491/2015 UGC-BSR dated 11 August 2015) and also thankful to Research scholar Mr. Manohar Patil and Mr. Chandrashekar Patil (School of Chemical Sciences, NMU Jalgaon) for their kind help. Authors are grateful to Dr. Anil Lachke, Scientist NCL, Pune, India for their constant encouragement. Authors are indebted to University Grants Commission and Department of Science and Technology, India for making the research facilities available under the UGC-SAP and DST-FIST programs sanctioned to the School of Life Sciences.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflicts of interest.
- 1.Ju-Nam Y, Lead JR (2008) Manufactured nanoparticles: an overview of their chemistry, interactions and potential environmental implications. Sci Environ 400:396–414Google Scholar
- 7.Li X, Xu H, Chen ZS, Chen G (2011) Biosynthesis of nanoparticles by microorganisms and their applications. J Nanomater 1–16. https://doi.org/10.1155/2011/270974
- 14.El-Baz AF, El-Batal AI, Abomosalam FM, Tayel AA, Shetaia YM, Yang ST (2016) Extracellular biosynthesis of anti-candida silver nanoparticles using Monascus purpureus. J Microbiol 56:531–540Google Scholar
- 24.Srianta I, Zubaidah E, Estiasih T, Iuchi Y, Yamada M (2017) Antioxidant activity of pigments derived from Monascus purpureus-fermented rice, corn, and sorghum. Int Food Res J 24:1186–1191Google Scholar
- 26.El-Batal AI, El-Baz AF, Abo Mosalam FM, Tayel AA (2013) Gamma irradiation induces silver nanoparticles synthesis by Monascus purpureus. J Chem Pharm Res 5:1–15Google Scholar
- 34.Narasimha G, Praveen B, Mallikarjuna K, Deva Prasad Raju B (2011) Mushrooms (Agaricus bisporus) mediated biosynthesis of sliver nanoparticles, characterization and their antimicrobial activity. Int J Nano Dimens 2:29–36Google Scholar
- 36.Singh K, Panghal M, Kadyan S, Chaudhary U, Yadav JP (2014) Antibacterial activity of synthesized silver nanoparticles from Tinospora cordifolia against multi drug resistant strains of Pseudomonas aeruginosa isolated from burn patients. J Nanomed Nanotechnol 5:1Google Scholar
- 38.Durairaj R, Amirulhusni AN, Palanisamy NK, Mohd-Zain Z, Ping LJ (2012) Antibacterial effect of silver nanoparticles on multi drug resistant Pseudomonas aeruginosa. World Acad Sci Eng Technol 6:210–213Google Scholar
- 51.Vasileva P, Alexandrova T, Karadjova I (2017) Application of starch-stabilized silver nanoparticles as a colorimetric sensor for mercury(II) in 0.005 mol/L nitric acid. J Chem 1–9. https://doi.org/10.1155/2017/6897960