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Bioprocess and Biosystems Engineering

, Volume 42, Issue 4, pp 631–642 | Cite as

Optimization of fermentation conditions for carotenoid production in the radiation-resistant strain Deinococcus xibeiensis R13

  • Liqing Tian
  • Xian XuEmail author
  • Ling Jiang
  • Zhidong Zhang
  • He HuangEmail author
Research Paper
  • 169 Downloads

Abstract

Deinococcus xibeiensis R13 was isolated from an extreme environment in Xinjiang, China, and can resist gamma-radiation and UV-irradiation. In this study, D. xibeiensis R13 was shown to be capable of efficiently producing carotenoids in culture, and factors influencing its productivity were identified. The maximum carotenoid yield was observed at an initial temperature of 30 °C and pH 7.0 in the presence of fructose, tryptone at a C/N ratio of 1:5, and 10 µM Fe2+. The carotenoid yield under modified culture conditions was 6.64 mg/L after fermentation for 48 h, representing an increase of 84% compared to the original conditions. The biomass reached 7.22 g/L, which was 2.19-fold higher than under non-optimized conditions. The produced carotenoids were extracted from R13 and analyzed by UPLC-MS. This is the first study of carotenoid production by the new strain D. xibeiensis R13, which provides a new source for the microbial fermentation of natural carotenoids, and also provides a good reference for industrial production of other carotenoids and other terpenoid products.

Keywords

Deinococcus xibeiensis R13 Carotenoids Growth media Growth conditions Optimization 

Notes

Acknowledgements

This work was supported by the Joint Fund of the National Natural Science Foundation of China (U1603112), the National Natural Science Foundation of China (21776136, 21606129), the Program for Innovative Research Teams in Universities of Jiangsu Province (2015), and The Jiangsu Synergetic Innovation Center for Advanced Bio-Manufacture (No. XTC1810).

Supplementary material

449_2018_2069_MOESM1_ESM.doc (41 kb)
Supplementary material 1 (DOC 41 KB)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical EngineeringNanjing Tech UniversityNanjingChina
  2. 2.College of PharmacyNanjing Tech UniversityNanjingChina
  3. 3.College of Food Science and Light IndustryNanjing Tech UniversityNanjingChina
  4. 4.Institute of MicrobiologyXinjiang Academy of Agricultural Sciences, Xinjiang Uyghur Autonomous RegionUrumqiChina

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